Folia Parasitologica, vol. 49 (2002), issue 3

Folia Parasitologica 49[3] 239-242 (2002) | DOI: 10.14411/fp.2002.043

The enzymes of glycogen and trehalose catabolism from Hysterothylacium aduncum (Nematoda: Anisakidae)

Krystyna Żółtowska1, Elżbieta Łopieńska1, Jerzy Rokicki2, Małgorzata Dmitryjuk1
1 Department of Biochemistry, Faculty of Biology, University of Warmia and Mazury, ul. Żołnierska 14, 10-561 Olsztyn, Poland
2 Invertebrate Zoology Division, Gdańsk University, Al. Piłsudskiego 46, 81-378 Gdynia, Poland

The activity of α-amylase, glucoamylase, maltase, trehalase, glycogen phosphorylase and trehalose phosphorylase was measured in extracts from larval and adult Hysterothylacium aduncum (Rudolphi, 1802), parasitic nematode of marine fish. The content of glycogen and trehalose in the worm's body was also determined. Both the hydrolytic and phosphorolytic paths of sugar decomposition are present in H. aduncum. In the larvae glycogen was utilised mainly via the hydrolytic path. In the adults the activities of phosphorolytic enzymes were higher than in the larvae. In both stages the activity of trehalose phosphorylase is present. In adult nematodes it is uncommonly high. The dominating sugars in the adults were glucose and glycogen, while in the larvae it was trehalose.

Keywords: Nematoda, Hysterothylacium aduncum, enzymes, carbohydrate metabolism, glycogen, trehalose

Received: June 20, 2001; Accepted: February 6, 2002; Published: September 1, 2002


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