Folia Parasitologica, vol. 51 (2004), issue 2/3

Folia Parasitologica 51[2/3] 199-207 (2004) | DOI: 10.14411/fp.2004.023

Intraoligochaete development of Myxobolus intimus (Myxosporea: Myxobolidae), a gill myxosporean of the roach (Rutilus rutilus)

Orsolya Z. Rácz, Csaba Székely, Kálmán Molnár
Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18, H-1581 Budapest, Hungary

The infection with Myxobolus intimus Zaika, 1965 in the gills of the roach Rutilus rutilus (L.) from Lake Balaton was recorded in 28 out of the 39 fish examined. Developing and mature plasmodia were detected on the gills exclusively in the spring. The Myxobolus intimus infection was found only in 2- to 3-year-old fish. In histological sections, young plasmodia were found in capillaries of the secondary lamellae. More mature, round plasmodia 0.4-0.6 mm in diameter, deformed the respiratory lamellae. The intraoligochaete development of M. intimus was studied in experimentally infected oligochaetes. In two experiments, uninfected Tubifex tubifex Müller and Limnodrilus hoffmeisteri (Claparède) were exposed to mature myxospores of M. intimus. In both experiments, typical triactinospores developed in T. tubifex specimens but no infection was found in L. hoffmeisteri. In semithin sections, developmental stages, pansporocysts and actinospores, were found within the proliferated gut epithelium of T. tubifex. Triactinospores were first released from oligochaetes 37 and 58 days after initial exposure in the two experiments, respectively. Each triactinospore had three pyriform polar capsules and a cylindrical sporoplasm with 32 secondary cells. The spore body joined the 3 caudal projections with a moderately long style.

Keywords: Myxobolus intimus, myxospore, gill parasite, Rutilus rutilus, intraoligochaete development, Tubifex tubifex, triactinospore

Received: November 28, 2003; Accepted: April 2, 2004; Published: June 1, 2004


References

  1. BRINKHURST R.O. 1963: A guide for the identification of British aquatic Oligochaeta. Freshwater Biological Association Scientific Publication no. 22: 55 pp
  2. CSABA G., KOVACS-GAYER E., BEKESI L., BUCSEK M., SZAKOLCZAI J., MOLNAR K. 1984: Studies into the possible protozoan aetiology of swimbladder inflammation in carp fry. J. Fish Dis. 7: 39-56 Go to original source...
  3. DYKOVA I., LOMJ. 1982: Sphaerospora renicola n. sp., a myxosporean from carp kidney, and its pathogenicity. Z. Parasitenkd. 68: 259-268 Go to original source... Go to PubMed...
  4. DYKOVA I., LOMJ., GRUPCHEVA G. 1987: Pathogenicity and some structural features of Myxidium rhodei (Myxozoa: Myxosporea) from the kidney of the roach (Rutilus rutilus). Dis. Aquat. Org. 2: 109-115 Go to original source...
  5. El-MANSY A., MOLNAR K. 1997a: Extrapiscine development of Myxobolus drjagini Akhmerov, 1954 (Myxosporea, Myxobolidae) in oligochaete alternative hosts. Acta Vet. Hung. 45: 427-438
  6. El-MANSY A., MOLNAR K. 1997b: Development of Myxobolus hungaricus (Myxosporea: Myxobolidae) in oligochaete alternate hosts. Dis. Aquat. Org. 31: 227-232 Go to original source...
  7. El-MANSY A., MOLNAR K., SZEKELY C. 1998: Development of Myxobolus portucalensis Saraiva et Molnar, 1990 (Myxosporea: Myxobolidae) in the oligochaete Tubifex tubifex (Mueller). Syst. Parasitol. 41: 95-103 Go to original source...
  8. El-MATBOULI M., HOFFMANN R.W. 1989: Experimental transmission of two Myxobolus spp. developing bisporogeny via tubificid worms. Parasitol. Res. 75: 461-464 Go to original source... Go to PubMed...
  9. El-MATBOULI M., HOFFMANN R.W. 1993: Myxobolus carassii Klokacheva, 1914 also requires an aquatic oligochaete, Tubifex tubifex as intermediate host in its life cycle. Bull. Eur. Assoc. Fish Pathol. 13: 189-192
  10. El-MATBOULI M., MCDOWELL E.M., ANTONIO D.B., ANDREE K.B., HEDRICK R.P. 1999: Effect of water temperature on the development, release and survival of the triactinomyxon stage of Myxobolus cerebralis in its oligochaete host. Int. J. Parasitol. 29: 627-641 Go to original source... Go to PubMed...
  11. ESZTERBAUER E., SZEKELY C., MOLNAR K., BASKA F. 2000: Development of Myxobolus bramae (Myxosporea: Myxobolidae) in oligochaete alternate host, Tubifex tubifex. J. Fish Dis. 23: 19-25 Go to original source...
  12. GILBERT M., GRANATH W. 2001: Persistent infection of Myxobolus cerebralis, the causative agent of salmonid whirling disease, in Tubifex tubifex. J. Parasitol. 87: 101- 107 Go to original source... Go to PubMed...
  13. KENT M.L., ANDREE K.B., BARTHOLOMEW J.L., ElMATBOULI M., DESSER S.S., DEVLIN R.H., FEIST S.W., HEDRICK R.P., HOFFMANN R.W., KHATTRA J., HALLET S.L., LESTER R.J.G., LONGSHAW M., PALENZUELA O., SIDDALL M.E., XIAO C. 2001: Recent advances in our knowledge on Myxozoa. J. Eukaryot. Microbiol. 48: 395-413 Go to original source... Go to PubMed...
  14. KENT M.L., WHITAKER D.J., MARGOLIS L. 1993: Transmission of Myxobolus arcticus Pugachev and Khokhlov, 1979, a myxosporean parasite of Pacific salmon, via a triactinomyxon from the aquatic oligochaete Stylodrilus heringianus (Lumbriculidae). Can. J. Zool. 71: 1207- 1211 Go to original source...
  15. LOMJ., MCGEORGE J., FEIST S.W., MORRIS D., ADAMS A. 1997: Guidelines for the uniform characterisation of the actinosporean stages of parasites of the phylum Myxozoa. Dis. Aquat. Org. 30: 1-9
  16. LOMJ., de PUYTORAC P. 1965: Observations sur l'ultrastructure des trophozoites de myxosporidies. C. R. Acad. Sci. (Paris) 260: 2588-2590
  17. MOLNAR K., El-MANSY A., SZEKELY C., BASKA F. 1999: Development of Myxobolus dispar (Myxosporea: Myxobolidae) in an oligochaete alternate host Tubifex tubifex. Folia Parasitol. 46: 15-21
  18. MOLNAR K., KOVACS-GAYER E. 1986: Observations on the intracellular and coelozoic developmental stages of Hoferellus cyprini (Doflein, 1898) (Myxosporea, Myxozoa) Parasitol. Hung. 19: 27-30
  19. MOLNAR K., SZEKELY C., CSABA G., LANG M., MAJOROS G. 2001: Pathological investigations on Lake Balaton fishes II. In: S. Mahunka and J. Banczerowski (Eds.), Results of the Lake Balaton Research in 2001. MTA, Budapest, pp. 160-169. (In Hungarian.)
  20. NOBLE E.R. 1944: Life cycles in the Myxosporidia. Q. Rev. Biol. 19: 213-235 Go to original source...
  21. RACZ O.Z., TIMM T. 2002: First report on the occurrence of actinosporean stages of fish myxosporeans (Myxozoa, Myxosporea) in Estonia. Acta Parasitol. 47: 190-195
  22. SHULMAN S.S. 1966: Myxosporidia of the fauna of the USSR. Nauka, Moscow, 504 pp. (In Russian.)
  23. SZEKELY C. 1997: Possible applications of video technology and digital image processing in fish parasitology: morphological examination of the groups Apicomplexa and Myxosporea-Actinosporea by video-technology. Bull. Eur. Assoc. Fish Pathol. 17: 81-82
  24. SZEKELY C., El-MANSY A., MOLNAR K., BASKA F. 1998: Development of Thelohanellus nikolskii (Myxosporea: Myxozoa) in oligochaete alternate hosts. Fish Pathol. 33: 107-114 Go to original source...
  25. SZEKELY C., MOLNAR K., ESZTERBAUER E., BASKA F. 1999: Experimental detection of the actinospores of Myxobolus pseudodispar (Myxosporea: Myxobolidae) in oligochaete alternate host. Dis. Aquat. Org. 38: 219-224 Go to original source... Go to PubMed...
  26. SZEKELY C., MOLNAR K., RACZ O. 2001: Complete developmental cycle of Myxobolus pseudodispar (Gorbunova) (Myxosporea: Myxobolidae). J. Fish Dis. 24: 461- 468 Go to original source...
  27. SZEKELY C., RACZ O., MOLNAR K., ESZTERBAUER E. 2002a: Development of Myxobolus macrocapsularis (Myxosporea: Myxobolidae) in an oligochaete alternate host, Tubifex tubifex. Dis. Aquat. Org. 48: 117-123 Go to original source... Go to PubMed...
  28. SZEKELY C., SITJA-BOBADILLA A., ALVAREZPELLITERO P. 2000: First report on the occurrence of an actinosporean stage (Myxozoa) in oligochaetes from Spanish freshwaters. Acta Vet. Hung. 48: 433-441 Go to original source... Go to PubMed...
  29. SZEKELY C., URAWA S., YOKOYAMA H. 2002b: Occurrence of actinosporean stages of myxosporeans in an inflow brook of a salmon hatchery in the Mena River System, Hokkaido, Japan. Dis. Aquat. Org. 49: 153-160 Go to original source... Go to PubMed...
  30. URAWA S. 1994: Life cycle of Myxobolus arcticus, a myxosporean parasite of salmonid fishes. International Symposium on Aquatic Animal Health, Seattle, 4-8 September 1994. Book of Abstracts, P-W-10.3
  31. USPENSKAYA A.V. 1984: Cytology of Myxosporidia. Nauka, Leningrad, 122 pp. (In Russian.)
  32. WOLF K., MARKIW M.E. 1984: Biology contravenes taxonomy in the Myxozoa: new discoveries show alternation of invertebrate and vertebrate hosts. Science 225: 1449- 1452 Go to original source... Go to PubMed...
  33. YOKOYAMA H. 1997: Transmission of Thelohanellus hovorkai Achmerov, 1960 (Myxozoa: Myxosporea) to common carp Cyprinus carpio through the alternate host. Syst. Parasitol. 36: 79-84 Go to original source...
  34. YOKOYAMA H., OGAWA K., WAKABAYASHI H. 1991: A new collection method of actinosporeans. A probable infective stage of myxosporeans to fishes from tubificids and experimental infection of goldfish with the actinosporean, Raabeia sp. Fish Pathol. 26: 133-138 Go to original source...
  35. ZAIKA V.E. 1965: [Parasite fauna of fishes from Lake Baikal.] Nauka, Moscow, 106 pp. (In Russian.)