Folia Parasitologica, vol. 52 (2005), issue 1/2

Folia Parasitologica 52[1/2] 145-157 (2005) | 10.14411/fp.2005.019

Chromosomal composition of the genome in the monomorphic diplokaryotic microsporidium Paranosema grylli: analysis by two-dimensional pulsed-field gel electrophoresis

Elena Nassonova1, Emmanuel Cornillot2, Guy Méténier2, Nina Agafonova1, Boris Kudryavtsev1, Sergei Skarlato1, Christian P. Vivarès2
1 Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, 194064 St. Petersburg, Russia
2 Equipe de Parasitologie Moléculaire et Cellulaire, UMR CNRS 6023, Université Blaise Pascal, 63177-Aubière cedex, France

The molecular karyotype of Paranosema grylli Sokolova, Seleznev, Dolgikh et Issi, 1994, a monomorphic diplokaryotic microsporidium, comprises numerous bright and faint bands of nonstoichiometric staining intensity. Restriction analysis of chromosomal DNAs by "karyotype and restriction display" 2-D PFGE has demonstrated that the complexity of molecular karyotype of P. grylli is related to the pronounced length polymorphism of homologous chromosomes. The background of this phenomenon is discussed in the context of ploidy state, reproductive strategy and population structure in this microsporidium. We propose that the remarkable size variation between homologous chromosomes in P. grylli may be a consequence of ectopic recombination at the chromosome extremities.

Keywords: Microsporidia, diplokaryon, molecular karyotype, KARD 2-D PFGE, chromosome-length polymorphism, ploidy, meiosis

Received: September 23, 2004; Accepted: March 22, 2005; Published: May 1, 2005


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