Folia Parasitologica, vol. 59 (2012), issue 1

Folia Parasitologica 59[1] 21-26 (2012) | 10.14411/fp.2012.004

Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP)

Xin Hu1, Chang-Wang Pan2, Ya-Fei Li1, Han Wang2, Feng Tan2
1 School of Medical Laboratory Science and School of Life Science, Wenzhou Medical College, Wenzhou, Zhejiang 325035, P. R. China
2 Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical College, Wenzhou, Zhejiang 325035, P. R. China

In this study, a loop-mediated isothermal amplification (LAMP) assay was established to detect Toxoplasma gondii DNA in mice infected with T. gondii PRU strain. This LAMP assay was based on the sequence of highly repetitive B1 gene. The detection limit of T. gondii LAMP assay was 1 pg of T. gondii DNA, which was evaluated using 10-fold serially diluted DNA of cultured parasites. The LAMP assay was also highly specific for T. gondii and able to detect T. gondii DNA in urine of mice treated with dexamethasone at 90 day post infection (p.i.), although this assay could not detect the DNA in mice urine 2-6 days p.i. These results demonstrated that LAMP is effective for evaluation of therapy effectiveness for T. gondii infection. The established LAMP assay may represent a useful and practical tool for the routine diagnosis and therapeutic evaluation of human toxoplasmosis.

Keywords: Toxoplasma gondii, toxoplasmosis, loop-mediated isothermal amplification (LAMP), urine, B1, mouse

Received: August 4, 2011; Accepted: December 11, 2011; Published: February 23, 2012


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