Folia Parasitologica, vol. 59 (2012), issue 2

Folia Parasitologica 59[2] 87-92 (2012) | 10.14411/fp.2012.013

Phylogenetic characterization of a microsporidium (Nosema sp.) isolated from the mulberry pest, Hemerophila atrilineata

Rui Guan1, Zhongyuan Shen1,2,3, Feng Zhu1, Darui Chen1, Jiao Zhang1, Jiange Hou1, Shinan Dong1, Xudong Tang1,2, Li Xu1,2
1 Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China
2 Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu Province, China
3 Key Laboratory of Genetic Improvement of Silkworm and Mulberry of Agricultural Ministry, Zhenjiang 212018, Jiangsu Province, China

Microsporidia are a group of obligate intracellular unicellular eukaryotes that can parasitize a wide variety of other eukaryotes ranging from protists to invertebrates and vertebrates. In this study, we examined the microsporidium Nosema sp. isolated from the mulberry pest, Hemerophila atrilineata Butler, 1881, named herein "Nosema sp. HA". The fresh spores were long oval in shape, 3.8 ± 0.4 μm in length and 1.9 ± 0.3 μm in width. Analysis of tissue infection of silkworm, Bombyx mori Linnaeus, 1758, indicated that the midgut, Malpighian tubules, muscle, fat body, silk glands, hemocytes, nerve tissue and gonads of silkworm were infected with Nosema sp. HA. The complete rRNA gene sequence of this microsporidium contained 4 305 base pairs (GenBank Accession JN882299), including the large subunit rRNA (2 492 bp), the internal transcribed spacer (187 bp), the small subunit rRNA (1 232 bp), the intergenic spacer (279 bp) and the 5S region (115 bp). The organization of the rRNA gene is 5'-LSU-ITS-SSU-IGS-5S-3'. Phylogenetic analysis, comparison of sequence identities and the arrangement in the rRNA gene subunits suggested that this isolate is separate from other Nosema species. Supporting information to this paper is available at http://folia.paru.cas.cz/suppl/2012-59-2-87.pdf

Keywords: Nosema sp. HA, gene organization, microsporidium, rRNA gene, phylogenetic analysis

Received: February 12, 2012; Accepted: May 4, 2012; Published: June 1, 2012


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References

  1. Baker M.D., Vossbrinck C.R., Maddox J.V., Undeen A.H. 1994: Phylogenetic relationships among Vairimorpha and Nosema species (Microspora) based on ribosomal RNA sequence data. J. Invertebr. Pathol. 64: 100-106 Go to original source... Go to PubMed...
  2. Bhat S.A., Bashir I., Kamili A.S. 2009: Microsporidiosis of silkworm, Bombyx mori L. (Lepidoptera - Bombycidae): a review. Afr. J. Agric. Res. 4: 1519-1523
  3. Canning E.U., Lom J., Dykova I. 1986: The Microsporidia of Vertebrates. Academic Press, London, 289 pp
  4. Corradi N., Haag K.L., Pombert J.F., Ebert D., Keeling P.J. 2009: Draft genome sequence of the Daphnia pathogen Octosporea bayeri: insights into the gene content of a large microsporidian genome and a model for host-parasite interactions. Genome Biol. 10: R106 Go to original source... Go to PubMed...
  5. Dong S.N., Shen Z.Y., Xu L., Zhu F. 2010: Sequence and phylogenetic analysis of SSU rRNA gene of five microsporidia. Curr. Microbiol. 60: 30-37 Go to original source... Go to PubMed...
  6. Fast N.M., Keeling P.J. 2001: Alpha and beta subunits of pyruvate dehydrogenase E1 from the microsporidian Nosema locustae: mitochondrion-derived carbon metabolism in microsporidia. Mol. Biochem. Parasitol. 117: 201-209 Go to original source... Go to PubMed...
  7. Franzen C., Muller A. 2001: Microsporidiosis: human diseases and diagnosis. Microbes Infect. 3: 389-400 Go to original source... Go to PubMed...
  8. Gatehouse H.S., Malone L.A. 1998: The ribosomal RNA gene region of Nosema apis (Microspora): DNA sequence for small and large subunit rRNA genes and evidence of a large tandem repeat unit size. J. Invertebr. Pathol. 71: 97-105 Go to original source... Go to PubMed...
  9. Huang W.F., Tsai S.J., Lo C.F., Yamane S., Wang C.H. 2004: The novel organization and complete sequence of the ribosomal RNA gene of Nosema bombycis. Fungal Genet. Biol. 41: 473-481 Go to original source... Go to PubMed...
  10. Huang W.F., Jiang J.H., Chen Y.W., Wang C.H. 2007: A Nosema ceranae isolate from the honeybee Apis mellifera. Apidologie 38: 30-37 Go to original source...
  11. Ironside J.E. 2007: Multiple losses of sex within a single genus of Microsporidia. BMC Evol. Biol. 7: e48 Go to original source... Go to PubMed...
  12. James T.Y., Kauff F., Schoch C.L., Matheny P.B., Hofstetter V., Cox C.J., Celio G., Gueidan C., Fraker E., Miadlikows ka J., Lumbsch H.T., Rauhut A., Reeb V., Arnold A.E., Am toft A., Stajich J.E., Hosaka K., Sung G.H., Johnson D., O'Rourke B., Crockett M., Binder M., Curtis J.M., Slot J.C., Wang Z., Wilson A.W., Schuessler A., Longcore J.E., O'Donnell K., Mozley-Standridge S., Porter D., Letcher P.M., Powell M.J., Taylor J.W., White M.M., Griffith G.W., Davies D.R., Humber R.A., Morton J.B., Sugiyama J., Rossman A.Y., Rogers J.D., Pfister D.H., Hewitt D., Hansen K., Hambleton S., Shoemaker R.A., Kohlmeyer J., Volkmann -Kohlmeyer B., Spotts R.A., Serdani M., Crous P.W., Hughes K.W., Matsuura K., Langer E., Langer G., Untereiner W.A., Luecking R., Buedel B., Geiser D.M., Aptroot A., Diederich P., Schmitt I., Schultz M., Yahr R., Hibbett D.S., Lutzoni F., McLaughlin D.J., Spatafora J.W., Vilgalys R. 2006: Reconstructing the early evolution of Fungi using a six-gene phylogeny. Nature 443: 818-822 Go to original source... Go to PubMed...
  13. Johny S., Kanginakudru S., Muralirangan M.C., Nagaraju J. 2006: Morphological and molecular characterization of a new microsporidian (Protozoa: Microsporidia) isolated from Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Parasitology 132: 803-814 Go to original source... Go to PubMed...
  14. Ku C.T., Wang C.Y., Tsai, Y.C., Tzeng C.C., Wang C.H. 2007: Phylogenetic analysis of two putative Nosema isolates from cruciferous lepidopteran pests in Taiwan. J. Invertebr. Pathol. 95: 71-76 Go to original source... Go to PubMed...
  15. Kyei -Poku G.K., Gauthier D., van -Frankenhuyzen K. 2008: Molecular data and phylogeny of Nosema infecting lepidopteran forest defoliators in the genera Choristoneura and Malacosoma. J. Eukaryot. Microbiol. 55: 51-58 Go to original source... Go to PubMed...
  16. Larsoon R. 1986: Ultrastructure, function, and classification of microsporidia. In: J.O. Corliss and D.J. Patterson (Eds.), Progress in Protistology. Vol. 1. Biopress, Bristol, England, pp. 325-390 Go to PubMed...
  17. Liu H., Pan G., Li T., Huang W., Luo B., Zhou Z. 2011: Ultrastructure, chromosomal karyotype, and molecular phylogeny of a new isolate of microsporidian Vairimorpha sp. BM (Microsporidia, Nosematidae) from Bombyx mori in China. Parasitol. Res. 110: 205-210 Go to original source... Go to PubMed...
  18. Mueller A., Trammer T., Chioralia G., Seitz H.M., Diehl V., Franzen C. 2000: Ribosomal RNA of Nosema algerae and phylogenetic relationship to other microsporidia. Parasitol. Res. 86: 18-23 Go to original source... Go to PubMed...
  19. Rao S.N., Muthulakshmi M., Kanginakudru S., Nagaraju J. 2004: Phylogenetic relationships of three new microsporidian isolates from the silkworm, Bombyx mori. J. Invertebr. Pathol. 86: 87-95 Go to original source... Go to PubMed...
  20. Refardt D., Mouton L. 2007: Reverse arrangement of rRNA subunits in the microsporidium Glugoides intestinalis. J. Eukaryot. Microbiol. 54: 83-85 Go to original source... Go to PubMed...
  21. Schuld M., Madel G., Schmuck R. 1999: Impact of Vairimorpha sp. (Microsporidia: Burnellidae) on Trichogramma chilonis (Hymenoptera, Trichogrammatidae), a hymenopteran parasitoid of the cabbage moth, Plutella xylostella (Lepidoptera, Yponomeutidae). J. Invertebr. Pathol. 74: 120-126 Go to original source... Go to PubMed...
  22. Solter L.F., Becnel J.J. 2000: Entomopathogenic microsporidia. In: L.A. Lacey and H. Kaya (Eds.), Field Manual of Techniques for the Evaluation of Entomopathogens. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 231-254
  23. Sprague V. 1977: Systematics of the Microsporidia . Plenum Press, New York, 510 pp Go to PubMed...
  24. Tamura K., Dudley J., Nei M., Kumar S. 2007: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596-1599 Go to original source... Go to PubMed...
  25. Tsai S.J., Huang W.F., Wang C.H. 2005: Complete sequence and gene organization of the Nosema spodopterae rRNA gene. J. Eukaryot. Microbiol. 52: 52-54 Go to original source... Go to PubMed...
  26. Tsai S.J., Kou G.H., Lo C.F., Wang C.H. 2002: Complete sequence and structure of ribosomal RNA gene of Heterosporis anguillarum. Dis. Aquat. Org. 49: 199-206 Go to original source... Go to PubMed...
  27. Tsai S.J., Lo C.F., Soichi Y., Wang C.H. 2003: The characterization of microsporidian isolates (Nosematidae: Nosema) from five important lepidopteran pests in Taiwan. J. Invertebr. Pathol. 83: 51-59 Go to original source... Go to PubMed...
  28. Tsai Y.C., Solter L.F., Wang C.Y., Fan H.S., Chang C.C., Wang C.H. 2009: Morphological and molecular studies of a microsporidium (Nosema sp.) isolated from the thee spot grass yellow butterfly, Eurema blanda arsakia (Lepidoptera: Pieridae). J. Invertebr. Pathol. 100: 85-93 Go to original source... Go to PubMed...
  29. Wang L.L., Chen K.P., Zhang Z., Yao Q., Gao G.T., Zhao Y. 2006: Phylogenetic analysis of Nosema antheraeae (Mi crosporidia) isolated from Chinese oak silkworm, Antheraea pernyi. J. Eukaryot. Microbiol. 53: 310-313 Go to original source... Go to PubMed...
  30. Wasson K., Peper R.L. 2000: Mammalian microsporidiosis. Vet. Pathol. 37: 113-128 Go to original source... Go to PubMed...
  31. Wittner M., Weiss L.M. 1999: The Microsporidia and Microsporidiosis. ASM Press, Washington, D.C., 553 pp
  32. Xu X., Shen Z., Zhu F., Tao H., Tang X., Xu L. 2011: Phylogenetic characterization of a microsporidium (Endoreticulatus sp. Zhenjiang) isolated from the silkworm, Bombyx mori. Parasitol. Res. 110: 815-819 Go to original source... Go to PubMed...
  33. Zhu F., Shen Z.Y., Guo X.J., Xu X.F., Tao H.P., Tang X.D., Xu L. 2011: A new isolate of Nosema sp. (Microsporidia, Nosematidae) from Phyllobrotica armata Baly (Coleoptera, Chrysomelidae) from China. J. Invert. Pathol. 106: 339-342 Go to original source... Go to PubMed...
  34. Zhu F., Shen Z.Y., Xu X.F., Tao H.P., Dong S.N., Tang X.D., Xu L. 2010: Phylogenetic analysis of complete rRNA gene sequence of Nosema philosamiae isolated from the lepidopteran Philosamia cynthia ricini. J. Eukaryot. Microbiol. 57: 294-296 Go to original source... Go to PubMed...
  35. Zhu X., Wittner M., Tanowitz H.B., Cali A., Weiss L.M. 1994: Ribosomal RNA sequences of Enterocytozoon bieneusi, Septata intestinalis and Ameson michaelis: phylogenetic construction and structural correspondence. J. Eukaryot. Microbiol. 41: 204-209 Go to original source... Go to PubMed...