Folia Parasitologica, vol. 59 (2012), issue 3

Folia Parasitologica 59[3] 195-208 (2012) | DOI: 10.14411/fp.2012.027

New data on the morphology of some Far-Eastern species of Rhabdochona (Nematoda: Rhabdochonidae), as revealed by SEM observation

František Moravec1, Aleksei V. Ermolenko2, Vladimir V. Besprozvannykh2, Tomáš Scholz1
1 Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic
2 Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, 159, Stoletiya Prospekt, 690022, Vladivostok, Russia

Specimens of the nematode genus Rhabdochona Railliet, 1916 (Rhabdochonidae) were collected during helminthological examination of four species of cyprinid fishes in two rivers of the Amur River basin in the Russian Far East (Primorsky Region) in June 2011. Detailed light microscopical (LM) and scanning electron microscopical (SEM) examinations (the latter used for the first time for the reported nematode species) of the available material revealed the presence of three inadequately described nominal species of this genus: R. (Rhabdochona) denudata (Dujardin, 1845) from the spotted steed Hemibarbus maculatus Bleeker (Gobioninae), and R. (Rhabdochona) longispicula Belous in Roytman, 1963 and R. (Globochonoides) coronacauda Belous, 1965 from Culter alburnus Basilewsky (Cultrinae) in the Ilistaya River. Detailed morphological study of these worms, especially SEM examination, made it possible to reveal some previously unreported morphological features (e.g., the presence of sublabia or the character of ventral precloacal ridges) and to confirm other taxonomically important characters such as the shape of deirids, number of anterior prostomal teeth, number and situation of lateral preanal and postanal papillae or the detailed structure of the crown-like formation on the female tail tip in R. coronacauda. Unidentifiable Rhabdochona (Rhabdochona) gravid females were recorded from the humpback Chanodichthys dabryi (Bleeker) (Cultrinae) in the Ilistaya River and from the Amur minnow Rhynchocypris lagowskii (Dybowski) (Leuciscinae) in the Komissarovka River.

Keywords: parasitic nematodes, Rhabdochona, freshwater fish, Chanodichthys, Culter, Hemibarbus, Russian Far East

Received: June 6, 2012; Accepted: July 17, 2012; Published: September 21, 2012


  1. Akhmerov A.K h . 1965: [Rhabdochona brevispicula nov. sp. a new nematode from fishes in Lake Khanka.] Trudy GELAN 15: 33-35. (In Russian.)
  2. Arai H.P., Mudry D.R. 1983: Protozoan and metazoan parasites of fishes from the headwaters of the Parsnip and McGregor rivers, British Columbia: a study of possible parasite transfaunations. Can. J. Fish. Aquat. Sci. 40: 1676-1684 Go to original source...
  3. Bauer O.N. ( Ed .) 1987: [Key to parasites of the USSR freshwater fish fauna. Part 3. Parasitic metazoans 2.] Nauka, Leningrad, 583 pp. (In Russian.)
  4. Belous E.V. 1953: [Parasitic worms of freshwater vertebrates of the Primorsky region.] PhD thesis of the All-Union K.I. Skryabin Institute of Helminthology (VIGIS), Moscow. (In Russian.)
  5. Belous E.V. 1965: [Nematodes of freshwater fishes from the Primorsky region.] In: V.A. Leonov, Yu.L. Mamaev, P.G. Oshmarin (Eds.), Paraziticheskie chervi domashnikh i dikikh zhivotnykh. Raboty po gelmintologii k 40-letiyu nauchnoy i pedagogicheskoy deyatelnosti professora A.A. Soboleva, Vladivostok, pp. 48-65. (In Russian.)
  6. Caspeta-Mandujano J.M., Moravec F. 2000: Two new intestinal nematodes of Profundulus labialis (Pisces, Cyprinodontidae) from fresh waters in Mexico. Acta Parasitol. 45: 332-339
  7. Chen C.L. ( Ed .) 1973: [An illustrated guide to the fish diseases and causative pathogenic fauna and flora in the Hubei Province.] Academia Sinica Press, Beijing, 456 pp. (In Chinese.)
  8. Dogel V.A., Akhmerov A.K h . 1959: [Nematodes of the Amur River.] Acta Hydrobiol. Sin. 3: 272-304. (In Chinese and Russian.)
  9. Ermolenko A.V. 1990: [Causative agents of infectious diseases of freshwater fishes in Primorsk Region.] Biologo-pochvennyi institut AN SSSR, Vladivostok, 56 pp. (In Russian.)
  10. Ermolenko A.V. 1992: [Parasites of fishes of freshwater reservoirs of the continental part of the Japan Sea basin.] Biologo-pochvennyi institut, DVO RAN, Vladivostok, 236 pp. (In Russian.)
  11. Ermolenko A.V., Butorina T.E. 1998: [Parasite fauna of cyprinid fishes of the subfamily Cultrinae in the Lake Khanka basin.] Parazitologiya 32: 156-166. (In Russian with Engl. summary.)
  12. Finogenova S.P. 1967: [New species of nematodes (Nematoda) from the Amur fishes.] Trudy Zool. Inst. AN SSSR 43: 93-98. (In Russian.)
  13. Finogenova S.P. 1971: [Round worms of fishes of the Amur River basin.] Trudy Zool. Inst. AN SSSR 25: 140-164. (In Russian.)
  14. Froese R., Pauly D. ( Eds .) 2012: FishBase. World Wide Web electronic publication,, version 05/2012
  15. Gusev A.V. 1969: [History of the fauna and attachment adaptations of the freshwater monogeneans of Eurasia and North America.] Parazitol. Sb. Zool. Inst. AN SSSR, Leningrad, 24: 106-127. (In Russian.)
  16. Hirasawa R., Urabe M. 2003: Ephemera strigata (Insecta: Ephemeroptera: Ephemeridae) is the intermediate host of the nematodes Rhabdochona denudata honshuensis and Rhabdochona coronacauda in Japan. J. Parasitol. 89: 617-620 Go to original source... Go to PubMed...
  17. Hirasawa R., Yuma M. 2003: Ephemera strigata imagoes are the likely source of a parasitic nematode infection of fish. Folia Parasitol. 50: 313-314 Go to original source... Go to PubMed...
  18. Moravec F. 1972: General characterization of the nematode genus Rhabdochona with a revision of the South American species. Acta Soc. Zool. Bohemoslov. 36: 29-46
  19. Moravec F. 1975: Reconstruction of the nematode genus Rhabdochona Railliet, 1916 with a review of the species parasitic in fishes of Europe and Asia. Studie CSAV No. 8. Academia, Prague, 104 pp
  20. Moravec F. 1994: Parasitic Nematodes of Freshwater Fishes of Europe. Academia and Kluwer Academic Publishers, Prague and Dordrecht, Boston, London, 473 pp
  21. Moravec F. 2007a: Some aspects of the taxonomy and biology of adult spirurine nematodes parasitic in fishes: a review. Folia Parasitol. 54: 239-257 Go to original source... Go to PubMed...
  22. Moravec F. 2007b: First experimental observations on the development of Rhabdochona denudata (Nematoda: Rhabdochonidae) in the intermediate host. Folia Parasitol. 54: 236-238 Go to original source... Go to PubMed...
  23. Moravec F. 2010: Some aspects of the taxonomy, biology, possible evolution and biogeography of nematodes of the spirurine genus Rhabdochona Railliet, 1916 (Rhabdochonidae, Thelazioidea). Acta Parasitol. 55: 144-160 Go to original source...
  24. Moravec F., Bilal S.J., Abdullah S.M.A. 2012a: Two species of Rhabdochona (Nematoda: Rhabdochonidae) from the cyprinid fish Luciobarbus kersin (Heckel) in northern Iraq, including R. (Globochona) kurdistanensis sp. n. Folia Parasitol. 59: 139-147 Go to original source... Go to PubMed...
  25. Moravec F., Ergens R. 1970: Nematodes from fishes and cyclostomes of Mongolia. Folia Parasitol. 17: 217-232
  26. Moravec F., Kamchoo K. 2012: Description of Rhabdochona (Globochona) rasborae sp. n. (Nematoda: Rhabdochonidae) from the freshwater cyprinid fish Rasbora paviana Tirant in southern Thailand. Folia Parasitol. 59: 209-215 Go to original source... Go to PubMed...
  27. Moravec F., Margolis L., Boyce N.P. 1981: Some nematodes of the genus Rhabdochona (Spirurida) from fishes of Japan. Acta Soc. Zool. Bohemoslov. 45: 277-290
  28. Moravec F., Nagasawa K. 1989: Observations on some nematodes parasitic in Japanese freshwater fishes. Folia Parasitol. 36: 127-141
  29. Moravec F., Nagasawa K. 1998: Helminth parasites of the rare endemic catfish, Liobagrus reini, in Japan. Folia Parasitol. 45: 283-294 Go to PubMed...
  30. Moravec F., Salgado -Maldonado G., Gonzalez -Solis D., Caspeta-Mandujano J.M. 2012b: Host-parasite relationships of Rhabdochona kidderi Pearse, 1936 (Nematoda: Rhabdochonidae) in fishes of the Lacantun River in the Lacandon rain forest of Chiapas State, southern Mexico, with a key to Mexican species of Rhabdochona Railliet, 1916. Syst. Parasitol. 82: 1-12 Go to original source... Go to PubMed...
  31. Moravec F., Scholz T., Ash A., Kar P.K. 2010: New data on the morphology and taxonomy of three species of Rhabdochona (Nematoda: Rhabdochonidae) parasitizing fishes in India. Folia Parasitol. 57: 295-306 Go to original source... Go to PubMed...
  32. Moravec F., Sey O. 1988: Nematodes of freshwater fishes from North Vietnam. Part 2. Thelazioidea, Physalopteroidea and Gnathostomatoidea. Acta Soc. Zool. Bohemoslov. 52: 176-191
  33. Moravec F., Sheeba S., Kumar A.B. 2012c: Rhabdochona (Rhabdochona) keralaensis sp. nov. (Rhabdochonidae) and some other nematodes in the Indian mottled eel Anguilla bengalensis bengalensis from India. Acta Parasitol. 57: 74-82 Go to original source... Go to PubMed...
  34. Moravec F., Yooyen T. 2011: Two new species of Rhabdochona (Nematoda: Rhabdochonidae) from freshwater fishes in Thailand. Folia Parasitol. 58: 224-232 Go to original source... Go to PubMed...
  35. Roytman V.A. 1962- 1963: [A zoogeographical characteristic of the helminth fauna of fishes of the Zeya River (the Amur River basin) and the ways of its formation.] Helminthologia 4: 404- 412. (In Russian with English, German and French summaries.)
  36. Roytman V.A. 1963: [Nematodes of the Zeya River basin.] Trudy GELAN 13: 253-300. (In Russian.)
  37. Roytman V.A., Trofimenko V.Ya . 1964: [Taxonomic importance of some morphological characters of Rhabdochona spp.] Helminthologia 5: 135-147. (In Russian with English and German summaries.)
  38. Shimazu T. 1996: Mayfly larvae, Ephemera japonica, as natural intermediate hosts of salmonid nematodes, Sterliadochona ephemeridarum and Rhabdochona oncorhynchi, in Japan. Jpn. J. Parasitol. 45: 167-172
  39. Shtein G.A. 1959: [On the questions of the life history and living conditions of the nematode Rhabdochona denudata (Dujardin, 1845).] Doklady AN SSSR 127: 1320-1321. (In Russian.)
  40. Skryabin K.I., Sobolev A.A., Ivashkin V.M. 1967: [Spirurata of Animals and Man and the Diseases Caused by Them.] Osnovy nematodologii 16. Nauka, Moscow, 624 pp. (In Russian.)
  41. Sterud E., Thoen E., Haugland O. 1998: First record of Rhabdochona oncorhynchi (Nematoda: Rhabdochonidae) from brown trout in Europe. Bull. Eur. Assoc. Fish Pathol. 18: 206-208
  42. Wang P.-Q., Zhao Y.-R., Wang X.-Y., Zhang J.-V. 1979: Report on some nematodes from vertebrate animals in Central and South China. Fujian Shida Xuebao 2: 78-92. (In Chinese with English summary.)