Folia Parasitologica, vol. 59 (2012), issue 3

Folia Parasitologica 59[3] 237-240 (2012) | 10.14411/fp.2012.032

Multilocus genotyping of Giardia duodenalis isolates from red deer (Cervus elaphus) and roe deer (Capreolus capreolus) from Poland

Piotr Solarczyk1, Anna C. Majewska1, Bożena Moskwa2, Władysław Cabaj2, Miroslawa Dabert3, Piotr Nowosad1
1 Department of Biology and Medical Parasitology, Poznań University of Medical Science, Faculty of Medicine I, 10 Fredry Street, 61-701 Poznań, Poland
2 Witold Stefański Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, Warsaw, Poland
3 Molecular Biology Techniques Laboratory, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland

A total of 181 faecal samples were collected from wild cervids in two regions of Poland. Giardia cysts were detected in one faecal specimen from red deer and in two samples from roe deer. Fragments of the β-giardin (bg) triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes were successfully amplified from the Giardia isolate obtained from red deer, whereas only amplicons of bg and gdh were obtained from Giardia isolates derived from two roe deer. The result of genotyping and phylogenetic analysis showed that the G. duodenalis isolate from red deer belonged to sub-assemblage AIII, which has never been identified in humans, whereas isolates from roe deer clustered within zoonotic sub-assemblage AI. Further studies are necessary to explain which Giardia assemblages and/or sub-assemblages occur in wild cervids in various regions of the world. Moreover, the impact of Giardia infection on the health of wild cervids should also be elucidated.

Keywords: Giardia, genotypes, molecular epidemiology, phylogeny, zoonoses

Received: March 9, 2012; Accepted: July 27, 2012; Published: September 21, 2012


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