Folia Parasitologica, vol. 61 (2014), issue 1

Folia Parasitologica 61[1] 34-36 (2014) | 10.14411/fp.2014.014

Detection of antibodies to Neospora caninum in moose (Alces alces): the first report in Europe

Bożena Moskwa1, Katarzyna Goździk1, Justyna Bień1, Aleksandra Kornacka1, Aleksandra Cybulska1, Katarína Reiterová2, Władysław Cabaj1
1 Witold Stefański Institute of Parasitology, Polish Academy of Sciences, Warsaw, Poland;
2 Institute of Parasitology, Slovak Academy of Sciences, Košice, Slovakia

Neospora caninum Dubey, Carpenter, Speer, Topper et Uggla, 1988 is a protozoan parasite originally reported as a major cause of bovine abortions worldwide. It is documented that the parasite is widely spread among non-carnivorous cervids. The purpose of this study was to investigate the seroprevalence of N. caninum in moose (Alces alces Linnaeus). Blood samples collected in 2010 and 2012 in the northeastern Poland were tested for antibodies to N. caninum by agglutination test (NAT), a commercial competitive screening enzyme-linked immunosorbent assay (cELISA) and enzyme-linked immunoassay (ELISA). Sera that gave a positive result were further investigated by western blot (WB) analysis to verify the presence of antibodies. Antibodies to N. caninum were detected in one of seven moose. The antibody titer was confirmed by NAT (1 : 1280), cELISA (I = 91%) and ELISA (OD = 0.736). The main immunodominant antigens detected by WB were 120, 70, 55, 35 and 16 kDa proteins. This is the first evidence of N. caninum seropositivity in moose living in a natural environment in Europe.

Keywords: Apicomplexa, elk, wildlife, serology, Poland

Received: July 25, 2013; Accepted: January 10, 2014; Prepublished online: January 10, 2014; Published: January 11, 2014


References

  1. Bartova E., Sedlak K., Pavlik I., Literak I. 2007: Prevalence of Neospora caninum and Toxoplasma gondii antibodies in wild ruminants from the countryside or captivity in the Czech Republic. J. Parasitol. 93: 1216-1218 Go to original source... Go to PubMed...
  2. Bien J., Moskwa B., Bogdaszewski M., Cabaj W. 2012: Detection of specific antibodies anti-Neospora caninum in the fallow deer (Dama dama). Res. Vet. Sci. 92: 96-98 Go to original source... Go to PubMed...
  3. Cabaj W., Moskwa B., Pastusiak K., Gill J. 2005: Antibodies to Neospora caninum in the blood of European bison (Bison bonasus bonasus L.) living in Poland. Vet. Parasitol. 128: 163-168 Go to original source... Go to PubMed...
  4. Dubey J.P., Carpenter J.L., Speer C.A., Topper M.J., Uggla A. 1988: Newly recognized fatal protozoan disease of dogs. J. Am. Vet. Med. Assoc. 192: 1269-1285 Go to PubMed...
  5. Dubey J.P., Jenkins M.C., Kwok O.C., Zink R.L., Michalski M.L., Ulrich V., Gill J., Carstensen M., Thulliez P. 2009: Seroprevalence of Neospora caninum and Toxoplasma gondii antibodies in white-tailed deer (Odocoileus virginianus) from Iowa and Minnesota using four serologic tests. Vet. Parasitol. 161: 330-334 Go to original source... Go to PubMed...
  6. Dubey J.P., Thulliez P. 2005: Prevalence of antibodies to Neospora caninum in wild animals. J. Parasitol. 91: 1217-1218 Go to original source... Go to PubMed...
  7. Gondim L.F., McAllister M.M., Mateus-Pinilla N.E., Pitt W.C, Mech L.D, Nelson M.E. 2004: Transmission of Neospora caninum between wild and domestic animals. J. Parasitol. 90: 1361-1365 Go to original source... Go to PubMed...
  8. Goodswen S.J., Kennedy P.J., Ellis J.T. 2013: A review of the infection, genetics, and evolution of Neospora caninum: from the past to the present. Infect. Genet. Evol. 13: 133-150 Go to original source... Go to PubMed...
  9. Gozdzik K., Jakubek E.B., Bjoerkman C., Bien J., Moskwa B., Cabaj W. 2010: Seroprevalence of Neospora caninum in free living and farmed red deer (Cervus elaphus) in Poland. Pol. J. Vet. Sci. 13: 117-120 Go to PubMed...
  10. Jokelainen P., Naereaho A., Knaapi S., Oksanen A., Rikula U., Sukura A. 2010: Toxoplasma gondii in wild cervids and sheep in Finland: north-south gradient in seroprevalence. Vet. Parasitol. 171: 331-336 Go to original source... Go to PubMed...
  11. Malmsten J., Jakubek E.B., Bjoerkman C. 2011: Prevalence of antibodies against Toxoplasma gondii and Neospora caninum in moose (Alces alces) and roe deer (Capreolus capreolus) in Sweden. Vet. Parasitol. 17: 275-280 Go to original source...
  12. Moskwa B., Bien J., Cabaj W., Korinkova K., Koudela B., Stefaniak J. 2009: The comparison of different ELISA procedures in detecting anti-Trichinella IgG in human infections. Vet. Parasitol. 159: 312-315 Go to original source... Go to PubMed...
  13. Romand S., Thulliez P., Dubey J.P. 1998: Direct agglutination test for serologic diagnosis of Neospora caninum infection. Parasitol. Res. 84: 50-53 Go to original source... Go to PubMed...
  14. Sedlak K., Bartova E. 2006: Seroprevalence of antibodies to Neospora caninum and Toxoplasma gondii in zoo animals. Vet. Parasitol. 136: 223-231 Go to original source... Go to PubMed...
  15. Stieve E., Beckmen K., Kania S.A., Widner A., Patton S. 2010: Neospora caninum and Toxoplasma gondii antibody prevalence in Alaska wildlife. J. Wildl. Dis. 46: 348-355 Go to original source... Go to PubMed...
  16. Vikoren T., Tharaldsen J., Fredriksen B., Handeland K. 2004: Prevalence of Toxoplasma gondii antibodies in wild red deer, roe deer, moose, and reindeer from Norway. Vet. Parasitol. 120: 159-169 Go to original source... Go to PubMed...
  17. Accepted 25 October 2013