Folia Parasitologica, vol. 62 (2015)

Folia Parasitologica 62:017 (2015) | DOI: 10.14411/fp.2015.017

The occurrence of metacercariae of Petasiger (Digenea: Echinostomatidae) in an unusual site, within the lateral line scales of cyprinid fishes

Kálmán Molnár1, David I. Gibson2, Gábor Cech3, Melitta Papp4, Petra Deák-Paulus4, Lajos Juhász5, Norbert Tóth5, Csaba Székely3
1 Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences - Fish Pathology and Parasitology, Budapest, Hungary;
2 Department of Life Sciences, Natural History Museum, London, United Kingdom;
3 Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary;
4 National Food Chain Safety Office - Veterinary Diagnostic Directorate, Budapest, Hungary;
5 University of Debrecen, Faculty of Agricultural and Food Sciences and Environmental Management, Department of Nature Conservation Zoology and Game Management, Debrecen, Hungary

During a regular veterinary inspection of fishes from Lake Balaton, Hungary, echinostomatid metacercariae (Digenea), with collar spines characteristic of species of the genera Petasiger Dietz, 1909 and Paryphostomum Dietz, 1909, were found in the lateral line scales of a roach Rutilus rutilus (Linnaeus), an apparently unique site. In a subsequent examination of 586 fishes from 20 different species, similar infections were found in 11 species. The infection was virtually restricted to the lateral line scales, other scales being infected only incidentally. These encysted metacercariae had 27 collar spines, including eight larger angle spines and 19 smaller dorsal spines arranged in two rows. Two types of metacercarial cyst were found. One type had a cyst diameter of 138-171 µm × 105-120 µm and three central dorsal spines that were larger than the remainder and tended to resemble the angle spines. The second type of metacercarial cyst had a diameter of 128-157 µm × 105-115 µm and all 19 dorsal spines of the metacercaria were of a similar size. ITS sequences of the second type of metacercaria exhibited a 100% similarity to sequences of two adult Petasiger phalacrocoracis (Yamaguti, 1939) specimens collected from the gut of Phalacrocorax carbo (Linnaeus) in Hungary and to P. phalacrocoracis deposited in the GenBank database. Sequences obtained from two metacercariae of the first type showed a 2.8-2.9 % difference from sequences of the second type of metacercaria and from those of adult specimens of P. phalacrocoracis from cormorants. Based on these results, the second type metacercaria is considered to be a larval stage of P. phalacrocoracis, but the identity of the first type is uncertain. The unusual location of these metacercariae in the lateral line scales is discussed in relation to their transmission.

Keywords: metacercaria, Cyprinidae, Lake Balaton, molecular identification, Kis-Balaton Reservoir

Received: April 18, 2014; Accepted: December 7, 2014; Published online: February 20, 2015


References

  1. DeLamater E.D., Courtenay W.R., Jr. 1973: Variations in structure of the lateral-line canal on scales of teleostean fishes. Z. Morph. Tiere 75: 259-266. Go to original source...
  2. Esmaeili H.R., Gholami Z. 2011: Scanning electron microscopy of the scale morphology in cyprinid fish, Rutilus frisii kutum Kamenskii, 1901 (Actinopterygii: Cyprinidae). Iranian J. Fisher. Sci. 10: 155-166.
  3. Faltýnková A., Gibson D.I., Kostadinova A. 2008: A revision of Petasiger Dietz, 1909 (Digenea: Echinostomatidae) and a key to its species. Syst. Parasitol. 71: 1-40. Go to original source... Go to PubMed...
  4. Galazzo D.E., Dayanandan S., Marcogliese D.J., McLaughlin J.D. 2002: Molecular systematics of some North American species of Diplostomum (Digenea) based on rDNA-sequence data and comparisons with European congeners. Can. J. Zool. 80: 2207-2217. Go to original source...
  5. Georgieva S., Kostadinova A., Skirnisson K. 2012: The life cycle of Petasiger islandicus Kostadinova & Skirmisson, 2007 (Digenea: Echinostomatidae) elucidated with the aid of molecular data. Syst. Parasitol. 82: 177-183. Go to original source... Go to PubMed...
  6. Jousson O., Bartoli P., Pawlowski J. 1999: Molecular identification of developmental stages in Opecoelidae (Digenea). Int. J. Parasitol. 29: 1853-1858. Go to original source... Go to PubMed...
  7. Karmanova E.M. 1971: [The life cycle of Petasiger neocomense (Trematoda, Echinostomatidae).] Trudy Gel'mintol. Lab. Akad. nauk SSSR 21: 31-35 (In Russian.)
  8. King P.H., Van As J.G. 2000: Morphology and life history of Petasiger variospinosus (Trematoda: Echinostomatidae) in the Free State, South Africa. J. Parasitol. 86: 312-318.
  9. Kostadinova A. 2005: Family Echinostomatidae. In: A. Jones, R.A. Bray and D.I. Gibson (Eds.), Key to the Trematoda. Vol. 2. CAB International, Wallingford and Natural History Museum, London, pp. 9-64.
  10. Kostadinova A., Chipev N. 1992: Experimental data on the life-cycle of Petasiger grandivesicularis Ishii, 1935 (Trematoda: Echinostomatidae). Syst. Parasitol. 23: 55-65. Go to original source...
  11. Kostadinova A., Vaucher C., Gibson D.I. 2002: Redescriptions of two echinostomes from birds in Paraguay, with comments on Drepanocephalus Dietz, 1909 and Paryphostomum Dietz, 1909 (Digenea: Echinostomatidae). Syst. Parasitol. 53: 147-158. Go to original source... Go to PubMed...
  12. Kotlán S. 1922: Beiträge zur Kentniss der Trematoden. Zool. Jahrb. Syst. 45: 565-576.
  13. Milne I., Wright F., Rowe G., Marshal D.F., Husmeier D., McGuire G. 2004: TOPALi: Software for Automatic Identification of Recombinant Sequences within DNA Multiple Alignments, Bioinformatics 20: 1806-1807. Go to original source... Go to PubMed...
  14. Molnár, K., Székely C. 1995: Parasitological survey of some important fish species of Lake Balaton. Parasitol. Hung. 28: 63-82.
  15. Murai E., Sulgostowska T., Matskási I., Mészáros F., Molnár K. 1983. Parasitic helminths of vertebrates (fishes, amphibians, reptiles and birds) in the Hortobagy National Park. In: S. Mahunka (Ed.), The Fauna of the Hortobágy National Park. Volume II. Akadémiai Kiadó, Budapest, pp. 15-30.
  16. Naąincová V., Scholz T., Moravec F. 1993: The life cycle of Paryphostomum radiatum (Dujardin, 1845) (Trematoda: Echinostomatidae), a parasite of cormorants. Folia Parasitol. 40: 193-201.
  17. Naąincová V., Scholz T., Moravec F. 1994: Redescription of Petasiger exaeretus Dietz, 1909 and P. phalacrocoracis (Yamaguti, 1939) (Trematoda: Echinostomatidae), parasites of cormorants. Syst Parasitol 27: 139-147. Go to original source... Go to PubMed...
  18. Székely C., Molnár K. 1997: Preliminary survey of the parasite fauna of some important fish species in the Upper-Reservoir of the Kis-Balaton System. Parasitol. Hung. 29-30: 45-54.
  19. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013: MEGA6: Molecular Evolutionary Genetics Analysis, version 6.0. Mol. Biol. Evol. 30: 2725-2729. Go to original source... Go to PubMed...
  20. Thompson J.D., Higgins D.G., Gibson T.J. 1994: CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22: 4673-4680. Go to original source... Go to PubMed...
  21. Tkach V.V., Pawlowski J., Sharpilo V.P. 2000: Molecular and morphological differentiation between species of the Plagiorchis vespertilionis group (Digenea, Plagiorchiidae) occurring in European bats, with a redescription of P. vespertilionis (Müller, 1780). Syst. Parasitol. 47: 9-22. Go to original source... Go to PubMed...
  22. Voronina E.P., Hughes D.R. 2011: Types and development pathways of lateral line scales in some teleost species. Acta Zool. 94: 154-166. Go to original source... Go to PubMed...