Folia Parasitologica, vol. 62 (2015)

Folia Parasitologica 62:018 (2015) | 10.14411/fp.2015.018

A new species of Wallinia Pearse, 1920 (Digenea: Allocreadiidae) in Astyanax mexicanus (Characidae) from Mexico revealed by morphology and sequences of the 28S ribosomal RNA gene

Gerardo Pérez-Ponce de León1, Ulises Razo-Mendivil2, Berenit Mendoza-Garfias1, Miguel Rubio-Godoy2, Anindo Choudhury3
1 Laboratorio de Helmintología, Instituto de Biología, Universidad Nacional Autónoma de México, México, D.F., Mexico;
2 Instituto de Ecología, Red de Biología Evolutiva, Xalapa, Veracruz, Mexico;
3 Division of Natural Sciences, St. Norbert College, De Pere, Wisconsin, USA

Wallinia mexicana sp. n. is described from the Mexican tetra, Astyanax mexicanus (De Filippi) (Characidae Weitzman), from two localities in northern Mexico. The new species can be distinguished from the two congeneric species, described from small-bodied characids in South and Central America, mainly by the posterior extent of the vitelline follicles (halfway between the posterior testis and the end of the caeca), by having a larger oesophagus, testes that are always oblique, and eye-spot remnants. The distinct status of the new species was confirmed by molecular data (28S rRNA gene sequences). Phylogenetic analysis suggests the new species is the sister species of W. chavarriae Choudhury, Hartvigsen et Brooks, 2002 described from characids in northwestern Costa Rica. Additionally, genetic divergence between these congeners reached 3.3%, a value higher than that observed for closely related species pairs of allocreadiids for that molecular marker. Based on these new findings, recently published records of this new species as Magnivitellinum simplex Kloss, 1966 and Creptotrematina aguirrepequenoi Jiménez-Guzmán, 1973 in Astyanax mexicanus from Durango and San Luis Potosi states, respectively, are corrected.

Keywords: taxonomy, DNA, helminths, freshwater fish, North America

Received: June 10, 2014; Accepted: December 10, 2014; Published online: February 20, 2015


References

  1. Aguilar-Aguilar R., Martínez-Aquino A., Pérez-Rodríguez R., Pérez-Ponce de León G. 2014: Helminth parasites of freshwater fishes from Cuatrociénegas, Coahuila, in the Chihuahuan desert of Mexico: inventory and biogeographical implications. Int. Zool. 9: 328-339. Go to original source... Go to PubMed...
  2. Blasco-Costa I., Balbuena J.A., Kostadinova A., Olson P.D. 2009: Interrelationships of the Haploporinae (Digenea: Haploporidae): a molecular test of the taxonomic framework based on morphology. Parasitol. Int. 58: 263-269. Go to original source... Go to PubMed...
  3. Blasco-Costa I., Balbuena J.A., Raga J.A., Kostadinova A., Olson P.D. 2010: Molecules and morphology reveal crypticvariation among digeneans infecting sympatric mullets in the Mediterranean. Parasitology 137: 287-302. Go to original source... Go to PubMed...
  4. Choudhury A., Daverdin R.H., Brooks D.R. 2002: Wallinia chavarriae n. sp. (Trematoda: Macroderoididae) in Astyanax aeneus (Günther, 1860) and Bryconamericus scleroparius (Regan, 1908) (Osteichthyes: Characidae) from the Area de Conservación Guanacaste, Costa Rica. J. Parasitol. 88: 107-112.
  5. Curran S.S., Tkach V.V., Overstreet R.B. 2011: Phylogenetic affinities of Auriculostoma (Digenea: Allocreadiidae), with descriptions of two new species from Peru. J. Parasitol. 97: 661-670. Go to original source... Go to PubMed...
  6. Jiménez-Guzmán F. 1973: Tremátodos digéneos de peces dulceacuícolas de Nuevo León, México I: dos nuevas especies y un registro nuevo en el carácido Astyanax fasciatus mexicanus (Filippi). Cuad. Inst. Inv. Cien. UANL., Mex. 17: 1-19
  7. Mendoza-Franco E., Caspeta-Mandujano J.M., Salgado-Maldonado G. 2013: New species of Cacatuocotyle (Monogenoidea, Dactylogyridae) parasitizing the anus and the gill lamellae of Astyanax aeneus (Pisces, Ostariophysi: Characidae) from the Rio Lacantún basin in the Biosphere Reserve of Montes Azules, Chiapas, Mexico. Parasitol. Res. 112: 199-205. Go to original source... Go to PubMed...
  8. Miller R.R., Minckley W.L., Norris S.M. 2005: Freshwater Fishes of México. The University of Chicago Press, Chicago, Illinois, 652 pp.
  9. Mirande J.M. 2010: Phylogeny of the family Characidae (Teleostei: Characiformes): from characters to taxonomy. Neotrop. Ichthyol. 8: 385-568. Go to original source...
  10. Ornelas-García C.P., Domínguez-Domínguez O., Doadrio I. 2008: Evolutionary history of the fish genus Astyanax Baird & Girard (1854) (Actinopterygii, Characidae) in Mesoamerica reveals multiple morphological homoplasies. BMC Evol. Biol. 8: 340. Go to PubMed...
  11. Pearse A.S. 1920: The fishes of Lake Valencia, Venezuela. University of Wisconsin Studies in Science 1: 1-51.
  12. Pérez-Ponce de León G., Choudhury A. 2005: Biogeography of helminth parasites of freshwater fishes in México: the search for patterns and processes. J. Biogeogr. 32: 645-659. Go to original source...
  13. Pérez-Ponce de León G., Choudhury A. 2010: Parasite inventories and DNA-based taxonomy: lessons from helminths of freshwater fishes in a megadiverse country. J. Parasitol. 96: 236-244. Go to original source... Go to PubMed...
  14. Pérez-Ponce de León G., Choudhury A., Rosas-Valdéz R., Mejía-Madrid H. 2007: Systematic position of Wallinia spp. and Margotrema spp., parasites of Middle-American and Neotropical freshwater fishes based on 28S ribosomal RNA gene. Syst. Parasitol. 68: 49-55. Go to original source... Go to PubMed...
  15. Pérez-Ponce de León G., Mendoza-Garfias B., Rosas-Valdéz R., Choudhury A. 2013: New host and locality records of freshwater fish helminth parasites in river basins north of the Transmexican Volcanic Belt: another look at biogeographical patterns. Rev. Mex. Biodiv. 84: 556-562. Go to original source...
  16. Pérez-Ponce de León G. Razo-Mendivil, U., Rosas-Valdez R., Mendoza-Garfias B. Mejía-Madrid H. 2008: Description of a new species of Crassicutis Manter, 1936, parasite of Cichlasoma beani Jordan (Osteichthyes: Cichlidae) in Mexico, based on morphology and sequences of the ITS1 and 28S ribosomal RNA genes. J. Parasitol. 94: 257-263. Go to original source... Go to PubMed...
  17. Pérez-Ponce de León G., Rosas-Valdéz R., Aguilar-Aguilar R., Mendoza-Garfias B., Mendoza-Palmero C., García-Prieto L., Rojas A., Briosio-Aguilar R., Pérez-Rodríguez R., Domínguez-Domínguez, O. 2010: Helminth parasites of freshwater fishes in the Nazas River Basin, northern Mexico. Check List 6: 26-35.
  18. Razo-Mendivil U., Mendoza-Garfias B., Pérez-Ponce de León G., Rubio-Godoy, M. 2014a: A new species of Auriculostoma (Digenea: Allocreadiidae) in the Mexican tetra Astyanax mexicanus (Actinopterygii, Characidae) from central Veracruz, Mexico, described using morphological and molecular data. J. Parasitol. 100: 331-337. Go to original source... Go to PubMed...
  19. Razo-Mendivil U., Mendoza-Garfias B., Pérez-Ponce de León G., Rubio-Godoy, M. 2014b: Testing the systematic position and relationships of Paracreptotrema heterandriae within the Allocreadiidae through partial 28s rRNA gene sequences. J. Parasitol. 100: 537-541. Go to original source... Go to PubMed...
  20. Thatcher, V.E. 1993: Trematódeos Neotropicais. INPA/FUA, Manaus, Brazil, 553 pp.
  21. Tkach, V., Snyder S.D. 2008: Aptorchis glandularis n. sp. (Digenea: Plagiorchoidea) from the northwestern red-faced turtle, Emydura australis (Pleudodira: Chelidae) in the Kimberley, western Australia. J. Parasitol. 94: 918-924. Go to original source... Go to PubMed...
  22. Yamaguti, S. 1971: Synopsis of Digenetic Trematodes of Vertebrates. Vol. I. Keigaku Publishers, Tokyo, 1074 pp.