Folia Parasitologica, vol. 62 (2015)

Folia Parasitologica 62:064 (2015) | 10.14411/fp.2015.064

Presence of potential pathogenic genotypes of free-living amoebae isolated from sandboxes in children's playgrounds

Marcin Cholewiński, Piotr Solarczyk, Monika Derda, Agnieszka Wojtkowiak-Giera, Edward Hada¶
Department of Biology and Medical Parasitology, Faculty of Medicine I, Poznań University of Medical Sciences, Poznań, Poland

Some free-living amoebae are a potential threat to human health. The best known species are those of the genus Acanthamoeba Volkonsky, 1931, which cause Acanthamoeba keratitis, granulomatous amoebic encephalitis and other forms of tissue inflammation. The aim of the present study was to search for potential pathogenic genotypes of free-living amoeba in the sand in children's playgrounds. Our results confirmed that free-living amoebae were present in all examined playgrounds. Sequences of the 18S rDNA have shown that all isolated potentially pathogenic strains of amoebae belong to genotype T4 of Acanthamoeba. The potential pathogenicity of isolates was confirmed on mice. The presence of pathogenic amoebae in the examined sand may be a potential source of human infection.

Keywords: genotyping, Acanthamoeba, parasites, molecular study

Received: June 25, 2015; Accepted: October 15, 2015; Published online: December 9, 2015


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