Folia Parasitologica, vol. 63 (2016)

Folia Parasitologica 63:002 (2016) | DOI: 10.14411/fp.2016.002

Molecular phylogenetic confirmation of Gnathostoma spinigerum Owen, 1836 (Nematoda: Gnathostomatidae) in Laos and Thailand

Jurairat Jongthawin1,2, Pewpan M. Intapan1,2, Oranuch Sanpool1,2,3, Penchom Janwan4, Lakkhana Sadaow1,2, Tongjit Thanchomnang3, Sakhone Laymanivong2,5, Wanchai Maleewong1,2
1 Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand;
2 Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
3 Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand;
4 Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand;
5 Laboratory Unit, Centre of Malariology, Parasitology and Entomology, Ministry of Health, Lao People's Democratic Republic

We report the molecular-phylogenetic identification of larvae of the nematode genus Gnathostoma Owen, 1836 collected from a snake, Ptyas koros Schlegel, in Laos and adult worms from the stomach of a dog in Thailand. DNA was extracted and amplified targeting the partial cox1 gene and the ITS-2 region of ribosomal DNA. Phylogenetic analyses indicated that all five advanced third-stage larvae and seven adult worms were Gnathostoma spinigerum Owen, 1836. This is also the first molecular evidence of infection with G. spinigerum in a snake from Laos.

Keywords: ITS-2 rDNA, genotyping, parasitic nematode, fish-borne helminthoses, molecular taxonomy, South-East Asia

Received: September 11, 2015; Accepted: December 6, 2015; Published online: January 25, 2016


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