Folia Parasitologica, vol. 64 (2017)

Folia Parasitologica 64:001 (2017) | 10.14411/fp.2017.001

Myxobolus taibaiensis sp. n. (Myxozoa: Myxosporea) infecting the intestinal wall of common carp Cyprinus carpio Linnaeus in China

Xinhua Liu1,2, Congjie Hua1,2, Qianqian Zhang1, Yuanli Zhao1,2, Dong Zhang1,2, Jinyong Zhang1
1 Fish Diseases Laboratory, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China;
2 University of Chinese Academy of Science, Beijing, China

Myxobolus taibaiensis sp. n. was found in the inner intestinal wall of common carp, Cyprinus carpio Linnaeus, during the investigation of fish parasite fauna in Lake Taibai, located in the middle reach of the Yangtze River, China. The whitish ellipsoidal plasmodia, up to 2.9 mm long and 1.7 mm wide, developed in the circular muscle layer of the intestinal wall and produced significant compression into adjacent tissues, but no significant inflammatory responses were observed against this infection. Mature spores are oval in frontal view and lemon-like in lateral and apical view, averaging 10.2-11.2 µm (10.8 ± 0.2 µm) in length, 9.1-9.9 µm (9.6 ± 0.2 µm) in width and 6.1-6.6 µm (6.3 ± 0.1 µm) in thickness. Polar capsules are pyriform, equal in size, slightly converging anteriorly, measuring 4.4-5.4 µm (5.0 ± 0.2 µm) in length by 3.2-3.6 µm (3.4 ± 0.1 µm) in width. Polar filaments coiled with four to five turns and arranged perpendicular to the polar capsule length, measuring up to 106 µm. Myxobolus taibaiensis sp. n. is morphologically similar to Myxobolus rotundatus Achmerov, 1956 which also infects the inner wall of the intestine of common carp. However, the small subunit ribosomal DNA sequence identity was only 94%, generally beyond the intraspecies variation in the genus. Phylogenetically, this new species is sister to M. rotundatus and then clusters with M. shantungensis Hu, 1965 to form an independent common carp-infecting cluster within the Henneguya-Myxobolus clade.

Keywords: common carp, Cyprinidae, intestine, circular muscle layer, Yangtze River

Received: June 6, 2016; Accepted: November 10, 2016; Published online: January 10, 2017


References

  1. Atkinson S., Bartoąová P., Whipps C.M., Bartholomew J.L. 2015: Approaches for characterizing myxozoan species. In: B. Okamura, A. Gruhl and J.L. Bartholomew (Eds.), Myxozoan Evolution, Ecology and Development. Springer International Publishing, Cahm, pp. 111-123.
  2. Chen Q.L., Ma C.L. (Eds.) 1998: [Myxozoa: Myxosporea.] Science Press, Beijing, 993 pp. (In Chinese.)
  3. Dong C.F., Li X.Z., Weng S.P., Xie S.X., He J.G. 2013: Emergence of fatal European genotype CyHV-3/KHV in mainland China. Vet. Microbiol. 162: 239-244. Go to original source... Go to PubMed...
  4. Dyková I., Fiala I., Nie P. 2003: New data on Myxobolus longisporus (Myxozoa: Myxobolidae), a gill infecting parasite of carp, Cyprinus carpio haematopterus, from Chinese lakes. Folia Parasitol. 50: 263-268. Go to original source... Go to PubMed...
  5. Eiras J.C., Molnár K., Lu Y.S. 2005: Synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea: Myxobolidae). Syst. Parasitol. 61: 1-46. Go to original source... Go to PubMed...
  6. Eiras J.C., Zhang J.Y., Molnár K. 2014: Synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea: Myxobolidae) described between 2005 and 2014. Syst. Parasitol. 88: 11-36. Go to original source... Go to PubMed...
  7. Fiala I. 2006: The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int. J. Parasitol. 36: 1521-1534. Go to original source... Go to PubMed...
  8. Guindon S., Dufayard J.F., Lefort V., Anisimova M., Hordijk W., Gascuel O. 2010: New algorithms and methods to estimate maximum likelihood phylogenies: assessing the performance of PhyML 3.0. Syst. Biol. 59: 307-321. Go to original source... Go to PubMed...
  9. Hall T.A. 1999: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp Ser. 41: 95-98.
  10. Jin H.B. 2007: [Innovative culturing of lake method making high-yield fish product.] Fish. Guide Rich. 8: 14-15. (In Chinese.)
  11. Lin J.R., Chen Y.D., Guo R. 2014: [China Fishery Statistics Yearbook.] China Agriculture Press, Beijing, 521 pp. (In Chinese.)
  12. Liu Y. 2014: [Taxonomy of the genus Myxobolus (Myxozoa: Myxosporea) with identification of some Myxobolus species in China.] Huazhong Agriculture University, PhD thesis, 126 pp. (In Chinese.)
  13. Liu X.H., Zhang J,Y., Batueva M.D., Voronin V.N. 2016a: Supplemental description and molecular characterization of Myxobolus miyairii Kudo, 1919 (Myxosporea: Myxobolidae) infecting intestine of Amur catfish (Silurus asotus). Parasitol. Res. 115: 1547-1556. Go to original source... Go to PubMed...
  14. Liu Y., Whipps C.M., Gu Z.M., Huang M.J., He L.Y., Molnár K. 2013: Myxobolus musseliusae (Myxozoa: Myxobolidae) from the gills of common carp Cyprinus carpio and revision of Myxobolus dispar recorded in China. Parasitol. Res. 112: 289-296. Go to original source... Go to PubMed...
  15. Liu Y., Whipps C.M., Gu Z.M., Zeng L.B. 2010: Myxobolus turpisrotundus (Myxosporea: Bivalvulida) spores with caudal appendages: investigating the validity of the genus Henneguya with morphological and molecular evidence. Parasitol. Res. 107: 699-706. Go to original source... Go to PubMed...
  16. Liu X.H., Yuan S., Zhao Y.L., Fang P., Chen H., Zhang J.Y. 2016b: Morphological and molecular characterization of Myxobolus sheyangensis n. sp. (Myxosporea: Myxobolidae) with intralamellar sporulation in allogynogenetic gibel carp Carassius auratus gibelio (Bloch) in China. Parasitol. Res. 115: 3567-3574. Go to original source... Go to PubMed...
  17. Lom J., Dyková I. 2006: Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species, Folia Parasitol. 53: 1-36. Go to original source... Go to PubMed...
  18. Molnár K. 2002: Site preference of fish myxosporeans in the gill. Dis Aquat Org. 48: 197-207. Go to original source... Go to PubMed...
  19. Molnár K., Eszterbauer E., Marton S., Székely C. 2009: Myxobolus erythrophthalmi sp. n. and Myxobolus shaharomae sp. n. (Myxozoa: Myxobolidae) from the internal organs of rudd, Scardinius erythrophthalmus (L.), and bleak, Alburnus alburnus (L.). J. Fish Dis. 32: 219-231. Go to original source... Go to PubMed...
  20. Molnár K., Marton S., Eszterbauer E., Székely C. 2006: Comparative morphological and molecular studies on Myxobolus spp. infecting chub from the River Danube, Hungary, and description of M. muellericus sp. n. Dis. Aquat. Org. 73: 49-61. Go to original source... Go to PubMed...
  21. Page R.D.M. 1996: TREEVIEW: an application to display phylogenetic trees on personal computers. Comput. Appl. Biosci. 12: 357-358. Go to PubMed...
  22. Posada D. 2008: jModelTest: phylogenetic model averaging. Mol. Biol. Evol. 25: 1253-1256. Go to original source... Go to PubMed...
  23. Ronquist F., Huelsenbeck J.P. 2003: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574. Go to original source... Go to PubMed...
  24. Székely C., Atkinson S.D., Molnár K., Egyed L., Guyányi A., Cech G. 2016: A synopsis of records of myxozoan parasites (Cnidaria: Myxozoa) from shrews, with additional data on Soricimyxum fegati from common shrew Sorex araneus in Hungary and pygmy shrew Sorex minutus in Slovakia. Folia Parasitol. 63: 021. Go to original source... Go to PubMed...
  25. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013: MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol. Biol Evol. 30: 2725-2729. Go to original source... Go to PubMed...
  26. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. 1997: The CLUSTAL-X window interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids Res. 25: 4876-4882. Go to original source... Go to PubMed...
  27. Whipps C.M., Adlard R.D., Bryant M.S., Lester R.J.G., Findlay V., Kent M.L. 2003: First report of three Kudoa species from eastern Australia: Kudoa thyrsites from mahi mahi (Coryphaena hippurus), Kudoa amamiensis and Kudoa minithyrsites n. sp. from sweeper (Pempheris ypsilychnus). J. Euk. Microbiol. 50: 215-219. Go to original source... Go to PubMed...
  28. Yokoyama H., Inoue D., Kumamaru A., Wakabayashi H. 1997: Myxobolus koi (Myxozoa: Myxosporea) forms large- and small-type "cysts" in the gills of common carp. Fish. Pathol. 32: 211-217. Go to original source...
  29. Zhai Y.H., Gu Z.M., Guo Q.X., Wu Z.Z., Wang H.M., Liu Y. 2016: New type of pathogenicity of Thelohanellus kitauei Egusa & Nakajima, 1981 infecting the skin of common carp Cyprinus carpio L.. Parasitol. Int. 65: 78-82. Go to original source... Go to PubMed...