Folia Parasitologica, vol. 30 (1983), issue 4

Continual cultivation of Toxoplasma gondii on HeLa cells.

A Valkoun

Folia Parasitologica 30[4] 289-294 (1983)

Continual cultivation of Toxoplasma gondii in monolayers of HeLa cells has been performed for 6 years. The toxoplasmas were then used for serial cultivation experiments. While searching for optimal conditions for toxoplasma inoculation it was found that infected HeLa cells which were scraped off the glass were a more suitable inoculum than those released by trypsinization. The optimal dose was one zoite per 12 host cells, medium level height at adsorption 2 mm and adsorption time 90 min at 37 degrees C. The concentration of sodium bicarbonate ranging from 8.7 to 26.1 mM had no effect on the multiplication of toxoplasmas. In serial passages in Roux...

Growth and transformation of Trypanosoma danilewskyi in a liquid medium.

H Rousková, J Kulda

Folia Parasitologica 30[4] 295-301 (1983)

The complex liquid medium L4NHS has proved suitable for obtaining trypomastigote-rich suspensions of the fish parasite Trypanosoma danilewskyi. Growth of T. danilewskyi strains in the medium has been characterized in test tube cultures at 25 degrees C. The cultures with an initial cell density of 1 x 10(5) x ml01 grew exponentially for 3 days with an average generation time of 15 h 17 min (strain BD) and 14 h 25 min (strain BZ). Both strains remained in the stationary phase for another 7-10 days. Total growth amounted to 1.3-1.6 x 10(7) cells x ml-1. The trypanosomes in culture were polymorphic (epimastigotes, broad trypomastigotes, slender trypomastigotes)....

Evaluations of antibody levels in Toxoplasma infection by the immunofluorescent antibody test and ELISA test.

M E Azab, M A Rifaat, H M Khalil, E H Safer, M K Nabaweya

Folia Parasitologica 30[4] 303-307 (1983)

In this study 292 sera were screened by the IFAT. 46 sera with variable IFAT titres were tested with ELISA Reagent Set and microtitration tests. A comparative evaluation of the specificity and reliability of ELISA method with that of IFAT for the detection of Toxoplasma infection was done.

PARANOPLOCEPHALA-CAMPESTRIS (CHOLODKOWSKY, 1913) - PARASITE OF RODENTS OF THE FAMILY ARVICOLIDAE IN CZECHOSLOVAKIA

F TENORA, C VAUCHER

Folia Parasitologica 30[4] 307 (1983)

Choniagnium algericum sp. n. (Nematoda: Strongylidae) from the intestine of African elephant, Loxodonta africana (Blumenbach, 1779).

J Prokopic, D Hulínská, Z Záhor

Folia Parasitologica 30[4] 309 (1983)

Nematodes of the genus Choniagnium were found in African elephant (Loxodonta africana) from Algerian Zoological Garden. On the basis of studies in light and scanning electron microscope these nematodes were identified as a new species, Choniagnium algericum sp. n.

THE INDIA INK IMMUNOREACTION OR CARBON IMMUNOASSAY IN THE DIAGNOSIS OF HUMAN TOXOPLASMOSIS PERFORMED WITHOUT USE OF COMPLEMENT

J CHALUPSKY

Folia Parasitologica 30[4] 311-312 (1983)

RHABDOCHONA-PUYLAERTI SP-N (NEMATODA, RHABDOCHONIDAE) RECORDED FROM THE AFRICAN VIPER CAUSUS-RHOMBEATUS (LICHTENSTEIN)

F MORAVEC

Folia Parasitologica 30[4] 313-317 (1983)

A survey of the results of studies on Gyrodactylus katharineri Malmberg, 1964 (Gyrodactylidae: Monogenea).

R Ergens

Folia Parasitologica 30[4] 319 (1983)

The hitherto obtained information on Gyrodactylus katharineri Malmberg, 1964 is summarized, concerning particularly its localization, host species spectrum and distribution in Eurasia. The morphogenesis and morphological-metrical variability of hard parts of opisthaptor are dealt with in detail. The pathogenicity of the parasite is stressed and methods for its control in fish-breeding farms are proposed.

Gyrodactylus strelkovi sp.n. (Monogenea: Gyrodactylidae). A new fish parasite from the basin of the River Amur.

R Ergens, V I Danilov

Folia Parasitologica 30[4] 328 (1983)

ARGENTOPHILIC INTEGUMENTARY STRUCTURES OF PLAGIORCHIS-LARICOLA SKRJABIN, 1924 CERCARIAE

P SAMNALIEV, V DIMITROV, J BUSTA

Folia Parasitologica 30[4] 329-334 (1983)

Morphology of Cysticercus bovis during its development.

J Schramlová, K Blazek

Folia Parasitologica 30[4] 335 (1983)

On day 14 p.i., C. bovis is a spherical formation without cavity (in neonatally infected calves, the cavity already starts to form). On day 21 p.i., a conical anlage of the scolex is formed and on day 23 p.i., it bears a conspicuous invagination of the tegument. On day 28 p.i., the scolex with spiral canal, sucker anlages and single calcareous bodies is developed. On day 42 p.i., the suckers are fully differentiated and the calcareous bodies in scolex parenchyma are distributed up to below the level of suckers. On day 55 p.i., the calcareous bodies are present in the morphologically differentiated scolex even below the suckers. The authors assume that...

Scanning electron microscopy of the trematode Hasstilesia ovis.

Z Zdárská, T N Soboleva, T A Bakke

Folia Parasitologica 30[4] 341 (1983)

The trematode Hasstilesia ovis parasitic in sheep was studied by scanning electron microscopy. A single type of sensory papillae was found on the surface of oral and ventral suckers and around the genital pore. The whole surface of body, except the oral and ventral suckers and vicinity of genital pore, is covered with spines. The mostly multipointed and rarely singlepointed spines are of variable size and distribution on different parts of body. They irritate mechanically the intestinal mucosa of the definitive host and thus increase the pathogenic effect of the trematode.

Ancylostoma caninum: a report on the peripheral eosinophilia in naive and immunized Swiss albino mice.

N P Kolhe, G N Johri

Folia Parasitologica 30[4] 345-349 (1983)

The number of peripheral eosinophils was counted in female Swiss albino mice on 1, 4, 9, 16 and 30 days after infection with various single (500, 1000 and 2000) and weekly repeated (500 + + 500 + 1000, 1000 + 1000 and 1000 + 1000 + 2000) doses of filariform Ancylostoma caninum larvae. The eosinophil response was significantly higher in infected than in uninfected mice and reached a peak on day 16 in naive and day 4 and 9 in immunized mice. Immunized mice were significantly more eosinophilic than the naive mice. An attempt has been made to correlate eosinophilia with immunity of mice to A. caninum larvae.

The effect of monoclonal antibodies on Schistosoma mansoni cercarial penetration of mouse skin.

S K Abdel-Hafez, D M Zodda, S M Phillips

Folia Parasitologica 30[4] 351-355 (1983)

Monoclonal antibodies (MA) were prepared by the fusion of spleen cells from C57BL/6J mice infected with Schistosoma mansoni with non-secreting SP2 myeloma cells. Clones of fused cells secreting MA reactive with the egg and cercarial antigens (as determined by the enzyme-linked immunosorbent assay), as well as with schistosomules (as determined by immunofluorescence), were injected into pristane "primed" mice to produce ascitic fluid. Cercariae were treated with MA by incubating them in individual MA or a pool of 3 or 4 MA (0.25 ml each) in form of ascitic fluid diluted in 10 ml of snail water for 60-90 minutes prior to snail exposure. Groups of 7-9...

A NEW EREYNETID NASAL MITE, NEOSPELEOGNATHOPSIS-(SPELEOMYOTIS)-TADARIDAE SP-N FROM UGANDA (ACARINA, PROSTIGMATA)

F DUSBABEK

Folia Parasitologica 30[4] 355-356 (1983)

THE LIFE-CYCLE OF ORNITHODOROS-TADARIDAE CERNY-ET-DUSBABEK (ARGASIDAE) UNDER LABORATORY CONDITIONS

E HONZAKOVA, V CERNY, M DANIEL

Folia Parasitologica 30[4] 357-361 (1983)

Small mammals as reservoirs and transmitters of leptospires in livestock-breeding farms and their surroundings.

Z Sebek, M Vlcek, J Sterba

Folia Parasitologica 30[4] 363-371 (1983)

From 1976 to 1978, we examined 1723 wild small mammals, representing 15 species, from three animal production farms and their surroundings in the Trebon basin, south Bohemia, in order to detect antibodies against leptospirosis. Antibodies were found against the serovars grippotyphosa, sorex-jalna, sejroe and/or istrica. The serological positivity was higher in wet grassland stands and fields bordering fishponds. The dominant serovar was grippotyphosa against which antibodies were detected in 8 species of exoanthropic and synanthropic mammals. Owing to the wide ecological valence of the main natural reservoir of L. grippotyphosa--Microtus arvalis, this...

Activities of some enzymes in the perienteric fluid of Ascaris suum.

J Zenka, J Prokopic

Folia Parasitologica 30[4] 373-376 (1983)

Activities of 12 enzymes (amylase, lipase, cholinesterase, nonspecific carboxyl esterase, lactate dehydrogenase (LDH), alkaline phosphatase, glutamate-oxalacetate transaminase (GOT), glutamate-pyruvate transaminase (GPT), gamma-glutamyl transferase (gamma-GT), leucine aminopeptidase (LAP), malate dehydrogenase (MDH) and peroxidase) were determined in the perienteric fluid and homogenate of Ascaris suum. With the exception of amylase, all activities were higher in the homogenate than in the perienteric fluid. The enzyme activities in the perienteric fluid were then compared with those in the human serum. Comparable activities were demonstrated for LDH,...

ANCYLODISCOIDINS (MONOGENEA, DACTYLOGYRIDAE) FROM 2 FRESH-WATER FISH SPECIES OF PENINSULAR MALAYSIA

L H LIM, J I FURTADO

Folia Parasitologica 30[4] 377-380 (1983)

New findings of mesostigmatic mites from rodents in the territory of Afghanistan.

V Bukva, A Amin

Folia Parasitologica 30[4] 381-383 (1983)

A survey is given of mesostigmatic mites collected in Afghanistan from 7 species of rodents. From a total of 11 mite species identified Laelaps echidninus, L. nuttalli, Haemogamasus gyrinodes and Androlaelaps longipes were found in Afghanistan for the first time.

FLASK FOR REARING IXODES-(EXOPALPIGER)-TRIANGULICEPS TICK IN LABORATORY

J NOSEK, O KOZUCH, P WEISMANN

Folia Parasitologica 30[4] 383 (1983)

Occurrence of mosquitoes Culex pipiens molestus in Prague.

F Rettich

Folia Parasitologica 30[4] 384 (1983)