Folia Parasitologica, vol. 36 (1989), issue 4

The pathogenicity of rabbit coccidium Eimeria coecicola Cheissin, 1947.

J Vítovec, M Pakandl

Folia Parasitologica 36[4] 289-293 (1989)

The pathogenicity of Eimeria coecicola for rabbits has been demonstrated in our experiments. The animals suffered from prolonged affection of appendix lasting at least 20 days. Pathological changes appeared with the development of merogony since day 4 post infection (DPI) and were characterized by an inflammatory infiltration and abundant pyogenic component in lamina propria, swelling and coalescence of upper parts of appendix mucosa above atrophied domes, where spaces filled with stagnating inflammatory exudate, endogenic stages of coccidia, and desquamated epithelia are formed. The alteration of the epithelium and exposure of the appendix lamina...

Starch gel electrophoresis of lactate dehydrogenase and glucose phosphate isomerase of poultry coccidia using the LKB multiphor.

J Kucera

Folia Parasitologica 36[4] 295-299 (1989)

A modification of thin-layer starch gel horizontal electrophoresis is described. The original method of Wraxall and Culliford (1986) is improved so that the preparation of starch gel is as simple as preparing the agarose gel. Thus the commercially supplied kits and instruments for the agarose gel electrophoresis can be also used for the starch gel electrophoresis. Furthermore, a method of preparing the permanent dry enzymograms from the starch gels with visualized enzymes is presented. The described procedure was used in the LDH and GPI analyses of poultry coccidia.

Metabolic differentiation of bloodstream forms of Trypanosoma brucei brucei into procyclic forms in hemin-depleted medium and in the presence of respiratory inhibitors.

A Markos, A Blahůsková, M Kalous

Folia Parasitologica 36[4] 301-306 (1989)

Bloodforms of Trypanosoma brucei brucei (STIB 247) differentiated in vitro into procyclic forms as described in the accompanying paper (Markos et al. 1989). The importance of the respiratory chain for the process was tested by the inhibition of its development (omission of hemin from the medium) or function (respiratory inhibitors). In the absence of hemin, all enzyme markers of the procyclic state, except for hemoproteins, developed to 50-70 per cent of control values. The presence of hemin is therefore not essential for the onset of differentiation, although the process cannot be completed under hemin limitation. Addition of 1 mumole.dm-3 KCN, 10...

Ultrastructural study of the glandular system and its participation in tegument formation in Echinostoma revolutum cercaria.

Z Zdárská

Folia Parasitologica 36[4] 307-312 (1989)

Six types of gland cells occurring in the body of developing Echinostoma revolutum cercaria are described at ultrastructural level. Three types of the gland cells, lateral, ventral and dorsal, release their secretion into the tegument of cercaria during its development in the media. Another three types, paraoesophageal, penetration and proper cytogenic gland cells are preserved in the body of the free-swimming cercaria. The paraoesophageal gland cells open on the tegument surface. Secretory granules of all types of gland cells and stratification of the body tegument arising by gradual release of the contents of the above mentioned three types of gland...

Contribution to the developmental cycle and taxonomy of Neoglyphe sobolevi Shaldybin, 1953 (Trematoda: Omphalometridae).

V Nasincová, J Busta, T A Krasnolobova

Folia Parasitologica 36[4] 313-319 (1989)

In Europe, the life cycle of the trematode N. sobolevi was studied for the first time. Following developmental stages are described in detail: daughter sporocysts, cercariae, metacercariae, adults. Snails Galba corvus and Lymnaea stagnalis were found to be the first intermediate hosts in nature. Metacercariae were obtained experimentally from larvae of the mosquitoes Culex pipiens molestus, adults from the intestine of hamsters (Mesocricetus auratus) and mice (Mus musculus). This is the first finding of this species in Czechoslovakia. The stability of taxonomically important features and comparison with closely related species is discussed.

Microhabitats of monogenean gill parasites on European eel (Anguilla anguilla).

K Buchmann

Folia Parasitologica 36[4] 321-329 (1989)

The distribution of adult Pseudodactylogyrus anquillae and P. bini and postlarvae was recorded on glass-eels and pigmented eels of different size and infection intensity. The two species occupied different microhabitats on the host's gill apparatus but the preferred sites depended partly on host size. Postlarval migration was indicated. Mortality of young parasites, reinforcement of reproductive barriers and competition seems to be less likely explanations of the spatial distribution.

Influence of thymic preparations on the result of experimental infection with Taenia crassiceps (Zeder, 1800) in ICR mice.

J Hermánek, J Prokopic

Folia Parasitologica 36[4] 331-340 (1989)

Thymalin (Thymarin) and T-activin--thymic preparations of polypeptidic character--were used for influencing the parasitic infection in a model system mouse--Taenia crassiceps. A single subcutaneous application of 100 micrograms of Thymalin per mouse at the day of infection resulted in a decrease (by 54.9%) in the number of cysticerci in peritoneal cavity of experimental mice compared with the controls. Administration of Thymalin with T. crassiceps larval homogenate at various intervals before and after infection resulted in a statistically significant increase of the level of specific antibodies in the serum of infected mice, this increase, however,...

A new method for selective diagnostic staining of hooks of echinococci, cysticerci and tapeworms in histological sections.

J Sterba, P Milácek, J Vítovec

Folia Parasitologica 36[4] 341-344 (1989)

This paper describes a new method for selective staining of hooks of echinococci, cysticerci and tapeworms in histological sections. The method is based on pre-staining in hematoxylin, then the preparations are stained overnight in dilute polychrome blue and differentiated with tartrazine in Cellosolve (Ethylene glycol monoethyl ether or Ethylene glycol monomethyl ether). The hooks stain blue, nuclei brown to brown-green, and cell plasma is yellow. The hooks stain very brightly, which enables the identification of parasite remnants in granulomas and scars. The intensity of staining depends on the grade of maturity and sclerotization of hooks and these...

Paraphilometroides nemipteri gen. et sp. n. (Nematoda: Philometridae) from the marine fish Nemipterus peronii (Valenciennes) from Malaysia.

F Moravec, F Shaharom-Harrison

Folia Parasitologica 36[4] 345-350 (1989)

A new nematode species, Paraphilometroides nemipteri sp. n. is described from the female specimens collected from the dorsal fin and operculum of the marine perciforme fish, Nemipterus peronii off Kuala Terengganu coastal waters in Malaysia. It considerably differs from all other species in Philometridae in the structure of the head end (presence of wide dorsal and ventral cephalic cuticular alae supported by special inner transverse muscular formations) and, therefore, a new genus Paraphilometroides gen. n. has been erected to accommodate it. Additional characteristic features of P. nemipteri are the presence of cuticular bosses on the body, eight...

Triaenophorus nodulosus and T. crassus in fish from northern Finland.

E T Valtonen, P Rintamäki, M Lappalainen

Folia Parasitologica 36[4] 351-370 (1989)

10,288 fish specimens of 34 species from three water bodies in northern Finland were studied mainly in 1978-1982 Triaenophorus nodulosus and T. crassus parasites. Six of the 31 species from the Bothnian Bay were found to harbour T. nodulosus plerocercoids, four of the 11 from the cold, oligotrophic Lake Yli-Kitka in northeastern Finland and only one of the 6 from the small, eutrophic Lake Kuivasjärvi. The ruff (Gymnocephalus cernuus) was most essential intermediate host in the lakes, as supported by stomach analysis of predator fish. Although the highest prevalences were found in adult burbot (Lota lota) in two of the areas, these fish are more likely...

Scanning electron microscopic study on Toxascaris transfuga (Rudolphi, 1819) (Nematoda).

F Tenora, J Mituch, I Hovorka

Folia Parasitologica 36[4] 371-374 (1989)

The authors present original observations on the species Toxascaris transfuga obtained by means of scanning electron microscopy. Attention was paid to the structure of head end, morphology of papillae of the head and abdominal end, specific morphological traits of cloacae edges and morphology of the egg surface. Presented are morphological criteria which apparently differentiate the species T. transfuga from T. leonina (Linstow, 1902). T. transfuga and T. multipapillata Kreis, 1938 seem to be conspecific.

Passive transfer of humoral resistance against adults of the tsetse fly, Glossina palpalis palpalis (Diptera, Glossinidae), in rabbits.

V Matha, S Lukes, T Soldán

Folia Parasitologica 36[4] 375-377 (1989)

The possibility of passive transfer of rabbit humoral immunity against tsetse fly bites was investigated for the first time. Partial immunity of recipient animals was achieved after two intravenous injections of 15 ml of serum from immunized (donor) rabbits during 48 hrs. This treatment induced an apparent increase of resistance in the passively immunized group of rabbits expressed as direct mortality ("killing effect") of sucking flies within the following 72 hr period. The immunological state of immune sera of both donors and recipients was examined by ELISA, using the water-soluble proteins of tsetse salivary glands as antigen. No direct correlation...

Results of arbovirological examination of birds of the family Hirundinidae in Czechoslovakia.

Z Juricová, Z Hubálek, J Halouzka, K Hudec, J Pellantová

Folia Parasitologica 36[4] 379-383 (1989)

Migratory birds (swallow, Hirundo rustica; sand martin, Riparia riparia; house martin, Delichon urbica) caught in southern Moravia (Czechoslovakia) in 1984-87 were examined for arbovirus infections. Isolation experiments were carried out using blood samples of 183 birds (52 swallows, 107 sand martins, and 24 house martins). The results were negative. Serological examinations of 136 birds (36 swallows, 86 sand martins, and 14 house martins) were made by haemagglutination-inhibition test (HIT) using 6 arboviral antigens of the genera Alphavirus (Sindbis--SIN) and Flavivirus (tick-borne encephalitis--TBE, West Nile--WN) and of the family Bunyaviridae...