Folia Parasitologica, vol. 38 (1991), issue 4

FLAGELLA NUMBER AMONG NAEGLERIA FLAGELLATES

D T JOHN, T B COLE, R A JOHN

Folia Parasitologica 38[4] 289-295 (1991)

Scanning electron microscopy was used to determine the number of flagella on the flagellates of Naegleria australiensis, N. fowleri, N. gruberi, and N. jadini. Although the majority of flagellates had 2 flagella, there was considerable variation among individual cells. The number of flagella per flagellate varied from 1-8, with 2.4 being the average number per cell. For the different species, the average number of flagella per cell ranged from 2.0 in N. jadini to 3.1 for N. australiensis. The greatest amount of variation occurred in N. australiensis, with only 43 % of the cells having 2 flagella. By contrast, 92 % of N. fowleri cells had 2 flagella....

UNUSEFULNESS OF CO2-BAITED TRAPS FOR COLLECTING BAT TICKS IN NATURE

A ESTRADA-PENA, J DE-LA-CRUZ

Folia Parasitologica 38[4] 295-296 (1991)

OCCURRENCE OF BLASTOCYSTIS SP IN PIGS

M PAKANDL

Folia Parasitologica 38[4] 297-301 (1991)

The occurrence of Blastocystis sp. was monitored in pip of various age from 5 pig farms in Southern Bohemia. Prevalence was determined especially via cultivation in modified nutrient broth LES. In sucking pigs up to 2 days old Blastocystis sp. was not found; from 3 days Blastocystis sp. persists throughout the pig life. In droppings and in cultures there both vacuolar and granular forms were observed. Ameboid forms were not found. In culture ultrastructure was observed by transmission electron microscopy. Individual cells of Blastocystis sp. contained a central body, nucleus, endoplasmic reticulum, rarely a Golgi complex and numerous mitochondria....

USE OF ELISA FOR THE DIAGNOSTICS OF OVINE SARCOCYSTOSIS

V SVOBODOVA, M NEVOLE

Folia Parasitologica 38[4] 303-308 (1991)

A modification of micro ELISA was used for the detection of specific antibodies in the diagnostics of ovine sarcocystosis. The results of ELISA compared to those of indirect fluorescence reaction (IFR) were identical in 94.7%. The titres of specific antibodies were higher in 70.8% of samples if ELISA was used. The coincidence of the two tests is statistically highly significant (P < 0.005). ELISA can be recommended for both experimental and routine examinations for ovine sarcocystosis.

SOME TREMATODES OF REPTILES FROM LAOS

T SCHOLZ, O DITRICH

Folia Parasitologica 38[4] 309-318 (1991)

In 51 reptiles of 17 species from Vientiane province in Laos, three species of trematodes, Paradistomum geckonum, Encyclometra bungara, and Postorchigenes majeedi were recorded for the first time. The snake Enhydris plumbea represents a new definitive host of E. bungara; this parasite has hitherto been recorded only in India. Mabuya multifasciata is a new definitive host of P. geckonum; Gekko gecko, Hemidactylus frenatus and Cosymbotus platyurus of P. majeedi. The study of P. geckonum, specimens from different hosts revealed great morphological and metrical variability within this species, particularly in the shape and size of body. These morphological...

AONCHOTHECA-MORAVECI SP-N (NEMATODA, TRICHURIDAE), A NEW NEMATODE FROM THE LONG-FINGERED BAT, MYOTIS-CAPACCINII, IN SPAIN

J G ESTEBAN, S MASCOMA, J L OLTRAFERRERO, P BOTELLA

Folia Parasitologica 38[4] 319-326 (1991)

A description is made of a new trichurid species of the subfamily Capillariinae, Aonchotheca moraveci sp. n, parasitizing the stomach of the long-fingered bat, Myotis capaccinii (Bonaparte, 1837), in Spain. The male presents two caudal lateral alae, a membranous caudal bursa supported by two forked expansions, a spicule 405-536-mu-m long and a non-spiny spicular sheath. The female has a vulvar appendage and the egg-shell surface presents a fine striation, only scarcely appreciable at the polar plugs.

MICROSPALACINAE, A NEW SUBFAMILY OF THE FEATHER MITE FAMILY ALLOPTIDAE GAUD (ACARINA, ANALGOIDEA)

W T ATYEO, J GAUD

Folia Parasitologica 38[4] 327-343 (1991)

Microspalacinae subfam. n. (Analgoidea, Alloptidae) is established for the feather mite genus Microspalax Megnin et Trouessart (= Connivelobus Dubinin, new synonymy). Morphologies of the species are described and illustrated with line drawings and SEM micrographs, and taxonomical comments are given for named species. The mites are known from shearwaters (Procellariidae) and stormpetrels (Hydrobatidae); host associations are discussed. The alloptid subfamily Thysanocercinae Atyeo et Peterson is elevated to familial rank.

THE ACARINIA AND NYCTERIBIDIA ZONES OF MINIOPTERUS-SCHREIBERSI KUHL (MAMMALIA, CHIROPTERA) IN THE NORTHEAST OF SPAIN

A ESTRADA-PENA, J SERRA-COBO

Folia Parasitologica 38[4] 345-354 (1991)

Acarinia and nycteribidia zones of the bat species Miniopterus schreibersi Kuhl are studied in an area of northeast of Spain, where captures over a period of five years have been carried out. The species that characterize the mite-tick spectrum are Ixodes (Pomerantzevella) simplex, Macronyssus longimanus, Macronyssus granulosus and Spinturnix psi, nycteribidium is determined mainly by the presence of Nycteribia schmidlii. The life cycle, prevalence, curves of parasitation, and other notes on these most abundant ectoparasites are provided, as well as observations on other ticks, mites, and nycteribiid species found on this bat.

POLYPLAX SPINULOSA INFESTATION AND ANTIBODY-RESPONSE IN VARIOUS STRAINS OF LABORATORY RATS

P VOLF

Folia Parasitologica 38[4] 355-362 (1991)

The degree and duration of Polyplax spinulosa Burm. infestation depend on the sex, age and genetic factors of the host. Various rat strains differ in the severity of lousiness. Host grooming is an important factor in the rat-louse relationship. After longlasting infestations rats become partially resistant. Serum antibody level depends mainly on the severity and the duration of the infestation, and follows the dynamics of louse population. Serum antibodies have no direct negative effect on lice.

TOLYPOCLADIUM-TERRICOLA SP-N A NEW MOSQUITO-KILLING SPECIES OF THE GENUS TOLYPOCLADIUM GAMS (HYPHOMYCETES)

J WEISER, V MATHA, A JEGOROV

Folia Parasitologica 38[4] 363-369 (1991)

A new entomopathogenic species of the genus Tolypocladium, T terricola is described from a soil sample from Finland. From other known Tolypocladium species, T. terricola differs in morphology, production of secondary metabolites and possession of relatively strong mosquitocidal activity. The fungus is characterized by broad oval conidia (2.5 x 2-mu-m) with one pointed end which are produced in grape-like clusters and are not firmly adherent. When treated with T. terricola, mosquito larvae show typical features of intoxication characterized by the concentration of larvae in the centre of cup, hanging by their siphons on the surface.

SCANNING ELECTRON-MICROSCOPE STUDIES ON PHILOMETRA (RANJHINEMA) BENINENSIS-OBIEKEZIE, 1986 (NEMATODA, PHILOMETRIDAE)

A I OBIEKEZIE, K ANDERS

Folia Parasitologica 38[4] 371-374 (1991)

The complement of cephalic papillae in the gravid females of Philometra (Ranjhinema) is Obiekezie, 1986 was investigated using scanning electron microscopy and the following distribution was observed: 8 fleshy lobes on the outer circle each with a pair Of equal-Sized papillae; 6 papillae on the inner circle grouped into 4 subdorsal and subventral papillae and 2 papillae on the lateral margin of the oral opening. Based on these observations, the subgeneric diagnosis of Ranjhinema Rasheed, 1963 is modified with a retention of its subgeneric status. SEM studies are further shown to be indispensable for the clarification of cephalic structures in the nematode...

PRELIMINARY OBSERVATION OF THE SUSCEPTIBILITY OF SOME FRESH-WATER SNAILS TO THE INFECTION WITH VARESTRONGYLUS-SAGITTATUS (NEMATODA, PROTOSTRONGYLIDAE)

P REZAC

Folia Parasitologica 38[4] 375-378 (1991)

Under laboratory conditions, the freshwater snails Galba truncatula (Mull.), Radix peregra (Mull.) and Planorbarius corneus (L.) have been successfully infected with the first stage larvae of the protostrongylid nematode Varestrongylus sagittatus (Mueller, 1890) Dougherty, 1945. The net rate of the infection (mean number of larvae per snail examined - 1/s) was 6.2 l/s in R. peregra and 3.5 l/s in P. corneus. G. truncatula was susceptible to the infection too (36.3 l/s). However, this result can be considered as pilot only, because of the low number of the specimens of this species examined. At 25-degrees-C 50 % of V. sagittatus larvae reached the 3-rd...

LARVICIDAL ACTIVITY OF ACTINOMYCETE ISOLATE AGAINST TOXOCARA-CANIS

ALDOORI M, ALTAE AA, JALIL S, HASSAN SA

Folia Parasitologica 38[4] 379-382 (1991)

A soil isolate of actinomycete, strain S-70, revealing presence of plasmid(s) was placed in the genus Streptomyces according to the wall composition and morphology. The toxicity of S-70 grown on two fermentation media, glucose-asparagine broth (GAB) and malt extract-yeast extract broth (MYE), against infective larvae of Toxocara canis was assessed. The use of mortality test has provided a sensitive and reproducible bioassay. The results suggest high larvicidal activity of the Streptomyces crude products examined being highly potent when using GAB culture medium.