Folia Parasitologica, vol. 46 (1999), issue 2

Lectin-like sequences in genome of Borrelia burgdorferi

Nataliia Rudenko, Maryna Golovchenko, Libor Grubhoffer

Folia Parasitologica 46[2] 81-90 (1999)

Using degenerative primers designed on the basis of known sequences of lectin genes from different sources a fragment of genomic DNA of Borrelia burgdorferi ( strain B31) that contained a lectin-like sequence was isolated, cloned and sequenced. The presence of an open reading frame of 268 amino acids (position 1501-2304 bp) and the computer analysis of the predicted amino acid sequence showed 37% of identity and 75% of homology over region of 25 amino acids with the legume lectin proteins, including erythroagglutinating phytohemagglutinin (PHA-E) and leucoagglutinating phytohemagglutinin (PHA-L). The further analysis of the predicted amino acid...

Cytokine response to infection with the microsporidian, Encephalitozoon cuniculi

Pavlína Braunfuchsová, Jan Kopecký, Oleg Ditrich, Břetislav Koudela

Folia Parasitologica 46[2] 91-95 (1999)

The production of three cytokines, interferon gamma (IFN-γ), interleukin 10 (IL-10) and interleukin 12 (IL-12), was measured after intraperitoneal infection of immunocompetent Balb/c mice and immunodeficient SCID mice with the microsporidian, Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923. High levels of IFN-γ were detected in ex vivo cultures of peritoneal exudate cells (PEC) of Balb/c mice, a lower, but earlier IFN-γ response was observed in PEC from SCID mice. The early IL-10 response was detected in ex vivo cultures of splenocytes from Balb/c but not from SCID mice, explaining a delay in the IFN-γ response...

Caryospora maxima sp. n. (Apicomplexa: Eimeriidae), new coccidium from Forskal\'s sand snake, Psammophis schokari (Serpentes: Colubridae) from lava desert of Jordan

David Modrý, Břetislav Koudela, Ratib M. Al-Oran, Zuhair S. Amr

Folia Parasitologica 46[2] 96-98 (1999)

A new coccidian parasite of the genus Caryospora Léger, 1904 is described from the colubrid snake Psammophis schokari (Forskål, 1775). Oocysts of Caryospora maxima sp. n. are spherical or subspherical, 43.0 (40.0-46.0) × 42.1 (40.0-44.0) µm, with smooth and bilayered oocyst wall ca. 2.0 µm thick. A micropyle, oocyst residuum and polar granule are absent. Sporocysts are broadly ellipsoidal, 21.3 (21.0-22.0) × 16.3 (16.0-17.0) µm, with smooth, colourless and unilayered sporocyst wall. Stieda and substieda bodies are present. Sporocyst residuum is present as compact mass of granules lying centrally between...

Extraintestinal stages of coccidia in liver of Schneider\'s skink Eumeces schneideri (Sauria: Scincidae) from Northern Egypt

Břetislav Koudela, David Modrý

Folia Parasitologica 46[2] 99-102 (1999)

An extraintestinal coccidian parasite was identified in Schneider's skinks Eumeces schneideri Daudin, 1802. Numerous tissue cysts were found in melanomacrophage aggregations in the liver of six of ten examined skinks. No tissue cysts were found in other tissues. Tissue cysts were 22-26 × 9-13 µm and contained a single sporozoite. Sporozoites were 10-13 × 2-4 µm, and contained a single nucleus, homogeneous inclusion and PAS positive granules, and were surrounded by PAS negative, 1.5-3.0 µm thick cyst wall. Transmission electron microscopy revealed that the tissue cyst wall was composed of granular material and the sporozoites...

Identification of surface proteins of trophic and cyst forms of Blastocystis hominis by biotin labeling

Joaquín Villar, José-Antonio Carbajal, María-Desamparados Lanuza, María-Rosa Navarro, Rafael

Folia Parasitologica 46[2] 103-106 (1999)

The surface proteins of trophic (vacuolar and ameboid forms) and cyst forms of two axenic Blastocystis hominis Brumpt, 1912 isolates were studied. The surface proteins of both forms were biotin-labeled and the soluble proteins from unlabeled and biotin-labeled cells were electrophoresed in 10% SDS-polyacrylamide gels under reducing conditions. The electrophoresed proteins from biotinylated cells were transferred to nitrocellulose membranes and the avidin-peroxidase-labeled complex was used to identify the surface proteins. In trophic forms, 26 of the 38 soluble proteins, with MW ranging between 30 and >200 kDa, were identified as surface...

Evidence of host blood feeding by the monogenean, Ancyrocephalus mogurndae (Monogenea: Ancyrocephalidae) from the gills of the mandarin fish, Siniperca chuatsi

Qian Gao, Pin Nie, Wei J. Yao

Folia Parasitologica 46[2] 107-110 (1999)

By examining iron contents, it is demonstrated that the monogenean Ancyrocephalus mogurndae (Yamaguti, 1940) feeds on the blood of its host, the mandarin fish Siniperca chuatsi (Basilewsky). The iron content and then the quantity of blood necessary to produce this amount of iron are found different in young and fully-matured worms. Young worms contain higher levels of iron and estimated amount of blood. It is suggested that A. mogurndae may start to feed on host blood as attached on gills, and the amount of blood ingested by young worms may vary from 0.01 to 1.00 µl before reproduction. The difference between young and fully-matured...

Post-cyclic transmission in Pomphorhynchus laevis (Acanthocephala)

Clive R. Kennedy

Folia Parasitologica 46[2] 111-116 (1999)

A series of laboratory experiments was conducted to investigate the possibility of post-cyclic transmission in Pomphorhynchus laevis (Müller, 1776). Rainbow trout Oncorhynchus mykiss (Walbaum) were exposed to P. laevis in naturally infected Cottus gobio Linnaeus, Noemacheilus barbatulus (Linnaeus), Phoxinus phoxinus (Linnaeus) and Leuciscus cephalus (Linnaeus) and sacrificed one month after infection. Post-cyclic transmission was possible from all four species even though they came from three families and differed in respect of their status and suitability as hosts of P. laevis. There was no selection...

Amphipod intermediate host of Polymorphus minutus (Acanthocephala), parasite of water birds, with notes on ultrastructure of host-parasite interface

Bahram Sayyaf Dezfuli, Luisa Giari

Folia Parasitologica 46[2] 117-122 (1999)

From November 1997 to June 1998, 3,118 specimens of Echinogammarus stammeri (Karaman, 1931) (Amphipoda) were collected from the River Brenta (Northern Italy) and examined for larval helminths. Larvae of Polymorphus minutus (Goeze, 1782) singly infected the hemocoel of 23 (0.74%) crustaceans; all these larvae were cystacanth stages. This is the first record of Polymorphus minutus in E. stammeri. Some cystacanths had their forebody and hindbody fully inverted. Parasites were bright orange in colour and each was surrounded by a thin acellular envelope. This envelope likely protects the developing parasite larva from cellular...

Morphology and taxonomy of Salvelinema species (Nematoda: Cystidicolidae), swimbladder parasites of Pacific area salmonids

František Moravec, 2and Kazuya Nagasawa

Folia Parasitologica 46[2] 123-131 (1999)

The morphology of two Salvelinema Trofimenko, 1962 species, S. salmonicola (Ishii, 1916) and S. walkeri (Ekbaum, 1935), swimbladder nematodes of salmonids, was studied in detail, including scanning electron microscopy (SEM), on the basis of newly collected materials from Oncorhynchus masou, O. mykiss and Salvelinus malma from Japan and from Oncorhynchus clarki, O. kisutch and O. nerka from North America (Vancouver Island, B.C., Canada and Gulf of Alaska). Both nematode species proved to be morphologically very similar, differing substantially in the numbers and arrangements of egg filaments....

Digenetic trematodes parasitic on Engraulis anchoita (Pisces: Engraulidae) from Argentina and Uruguay

Juan Tomás Timi, Sergio Roberto Martorelli, Norma Haydeé Sardella

Folia Parasitologica 46[2] 132-138 (1999)

A total of 2,086 specimens of Engraulis anchoita Hubbs et Marini (Pisces: Engraulidae), from waters off Argentina and Uruguay were examined for parasitic digeneans. Four species of digeneans were found, Parahemiurus merus (Linton, 1910) (adult) in the stomach; Lecithochirium microstomum Chandler, 1935 (non-ovigerous and ovigerous juveniles) in the swimbladder; Cardiocephaloides sp. (metacercaria) in the eyes and Rhipidocotyle sp. (metacercaria) in the musculature. These four species are recorded for the first time as parasites of E. anchoita.

First record of the genus Syncuaria (Nematoda: Acuariidae) in Argentina, with description of a new species

María Celina Digiani

Folia Parasitologica 46[2] 139-144 (1999)

The nematode genus Syncuaria Gilbert, 1927 (Acuariidae) is reported for the first time from Argentina with two species parasitic in Threskiornithidae. Syncuaria plegadisi sp. n. from the white-faced ibis, Plegadis chihi (Vieillot), is described and figured. The new species differs from known species of Syncuaria by the following characters: absence of lateral alae; cephalic cordons narrow, consisting of cuticular plates dorsally and ventrally invaginated forming a groove; presence of cephalic spines; spicules with simple, rounded distal ends; left spicule two to three times longer than right one; female tail dorsally bent...

Survey of entomopathogenic nematodes from the families Steinernematidae and Heterorhabditidae (Nematoda: Rhabditida) in the Czech Republic

Zdeněk Mráček, Stanislav Bečvář, Pavel Kindlmann

Folia Parasitologica 46[2] 145-148 (1999)

Soil samples were collected from the whole territory of the Czech Republic, and the presence of entomopathogenic nematodes from the families Steinernematidae and Heterorhabditidae was evaluated by Galleria traps method. Of the 342 samples studied, 53.8% were positive for entomopathogenic nematodes with only one positive for the heterorhabditid, Heterorhabditis megidis Poinar, Jackson et Klein, 1987. Of steinernematid species, Steinernema kraussei (Steiner, 1923), Steinernema feltiae (Filipjev, 1934), Steinernema affine (Bovien, 1937), Steinernema intermedium (Poinar, 1985), Steinernema bicornutum Tallosi,...

Parasitic copepods of the narrownose smooth-hound shark Mustelus schmitti (Chondrichthyes: Triakidae) from Argentina

Jorge A. Etchegoin, Verónica A. Ivanov

Folia Parasitologica 46[2] 149-153 (1999)

The parasitic copepod fauna of 182 specimens of Mustelus schmitti Springer from the coast of Mar del Plata, Argentina was investigated. Three species of parasitic copepods were identified: Nessipus orientalis Heller, 1865 from the buccal cavity, Perissopus oblongus (Wilson, 1908) from the edge of pectoral, pelvic, dorsal, anal and caudal fins and in claspers, and Lernaeopoda galei Krøyer, 1837 from the base of the pectoral fins. N. orientalis was most common being present the entire year, while P. oblongus and L. galei occurred seasonally with low prevalence and mean intensity. There were differences...

Exoerythrocystic stage of Haemoproteus ptyodactyli in the spleen of its host, the gecko Ptyodactylus hasselquistii

Ilan Paperna, Simcha Finkelman

Folia Parasitologica 46[2] 154 (1999)

Parasitophorous vacuole of intranuclear coccidia maintains membranous junctions with the host-cell cytoplasm

Ilan Paperna, Margarita Vilenkin

Folia Parasitologica 46[2] 155-156 (1999)

Encystment sites of matacercariae of Hypoderaeum conoideum (Trematoda: Echinostomatidae) in freshwater gastropods

Rafael Toledo, Carla Muñoz-Antolí, Mercedes Pérez, José-Guillermo Esteban

Folia Parasitologica 46[2] 157-158 (1999)

Comephoronema oschmarini (Nematoda: Cystidicolidae), a specific parasite of Lota lota (Pisces), occurs in western Europe

František Moravec, Vladimíra Hanzelová, Tomáš Scholz, Daniel Gerdeaux

Folia Parasitologica 46[2] 159-160 (1999)