Folia Parasitologica, vol. 48 (2001), issue 3

The life cycle of Haemogregarina bigemina (Adeleina: Haemogregarinidae) in South African hosts

Angela J. Davies, Nico J. Smit

Folia Parasitologica 48[3] 169-177 (2001) | DOI: 10.14411/fp.2001.029

Haemogregarina bigemina Laveran et Mesnil, 1901 was examined in marine fishes and the gnathiid isopod, Gnathia africana Barnard, 1914 in South Africa. Its development in fishes was similar to that described previously for this species. Gnathiids taken from fishes with H. bigemina, and prepared sequentially over 28 days post feeding (d.p.f.), contained stages of syzygy, immature and mature oocysts, sporozoites and merozoites of at least three types. Sporozoites, often five in number, formed from each oocyst from 9 d.p.f. First-generation merozoites appeared in small numbers at 11 d.p.f., arising from small, rounded meronts. Mature,...

The development and fine structure of Lankesterella cf. dicroglossi (Apicomplexa: Lankesterellidae) infecting frogs in Niger, West Africa

Ilan Paperna, Coralie Martin

Folia Parasitologica 48[3] 178-186 (2001) | DOI: 10.14411/fp.2001.030

One of four Hoplobatrachus occipitalis (Günther, 1859) frogs received from Niger, West Africa was heavily infected with Lankesterella blood and pre-erythrocytic stages. Infected blood and tissues from this frog were force-fed to the remaining three frogs. Two survived to necropsy on days 14 and 27 post-feeding and were found to be infected with gamogonic and oogonic stages, respectively. The source of infection is inconclusive, as a natural origin cannot be excluded. Microgamont, macrogamont, oocyst and sporozoite structure and fine structure are described and found to conform in general, but not in detail, to previous descriptions. Gamonts...

In vitro secretion of metabolic end-products by piscine haemoflagellates Cryptobia salmositica and C. bullocki (Kinetoplastida: Bodonidae) and the relationship of these products to the pH in the medium

Bernadette F. Ardelli, Patrick T.K. Woo

Folia Parasitologica 48[3] 187-191 (2001) | DOI: 10.14411/fp.2001.031

Pathogenic and nonpathogenic strains of Cryptobia salmositica Katz, 1951 and C. bullocki Strout, 1965 produced hydrogen peroxide, pyruvate and lactate under in vitro conditions in Minimum Essential Medium (MEM). As parasite number increased, the phenol red in the medium changed from red to yellow. This change was not associated with a decrease in pH, or an increase in pyruvate or lactate, but was correlated with an increased secretion of hydrogen peroxide. Parasites incubated at 10°C in medium at pH 6.0, 6.5, 7.0 and 7.3 were active for about 1 week with decreasing activity in the absence of serum. Parasites in saline (pH 6.0,...

Growth of Trachipleistophora hominis (Microsporidia: Pleistophoridae) in C2,C12 mouse myoblast cells and response to treatment with albendazole

Nathalie J. Lafranchi-Tristem, Alan Curry, Sarah A. Cheney, Elizabeth U. Canning

Folia Parasitologica 48[3] 192-200 (2001) | DOI: 10.14411/fp.2001.032

The microsporidium Trachipleistophora hominis Hollister, Canning, Weidner, Field, Kench et Marriott, 1996, originally isolated from human skeletal muscle cells, inhibited myotube formation from myoblasts when grown in a mouse myoblast cell line C2,C12. Uninfected cultures readily converted to myotubes. Albendazole, a drug with known antimicrosporidial activity, was tested against T. hominis in C2,C12 cells. The drug was added when infection had reached 75% of C2,C12 cells, a level comparable to that obtained in heavily infected muscle in vivo. Doses of 1 ng/ml and...

Chaetotaxy of the monogeneans Macrogyrodactylus clarii and M. congolensis from the gills and skin of the catfish Clarias gariepinus in Egypt, with a note on argentophilic elements in the nervous system

Mohammed M. El-Naggar, Safaa Z. Arafa, Sameer A. El-Abbassy, Graham C. Kearn

Folia Parasitologica 48[3] 201-208 (2001) | DOI: 10.14411/fp.2001.033

A comparison has been made between the chaetotaxy of the gyrodactylid monogeneans Macrogyrodactylus clarii Gussev, 1961 and M. congolensis (Prudhoe, 1957) Yamaguti, 1963 from the gills and skin, respectively, of the catfish Clarias gariepinus (Burchell) from the river Nile in Egypt. Bilaterally arranged argentophilic structures on the surface of these parasites are presumed to be sensilla and are more abundant in M. clarii than in M. congolensis especially on the ventral surface (124 vs. 66). In both species these sensilla are concentrated on the head lobes and in the pharyngeal region, but there are features of the...

Ultrastructure of spermiogenesis and spermatozoa of Discocotyle sagittata (Monogenea: Polyopisthocotylea: Discocotylinea)

Joanne Cable, Richard C. Tinsley

Folia Parasitologica 48[3] 209-216 (2001) | DOI: 10.14411/fp.2001.034

Ultrastructural analysis revealed that the spermatozoon of Discocotyle sagittata (Leuckart, 1842) is composed of two parallel axonemes, mitochondrion, nucleus and cortical microtubules. The nucleus, which occupies a central/distal position and has an unusual crescent-shaped profile, is slightly shorter than the mitochondrial rod. The two axonemes, which are of unequal length, and the cortical microtubules (up to 68 forming a continuous ring in the principal region) extend almost the entire length of the spermatozoon. A fold of the plasma membrane creates a unilateral flange or undulating membrane. Epifluorescence microscopy indicated that spermatogenesis...

A new monorchiid cercaria (Digenea) parasitising the purple clam Amiantis purpurata (Bivalvia: Veneridae) in the Southwest Atlantic Ocean, with notes on its gonadal effect

Florencia Cremonte, Marina Andrea Kroeck, Sergio Roberto Martorelli

Folia Parasitologica 48[3] 217-223 (2001) | DOI: 10.14411/fp.2001.035

An unnamed microcercous cercaria (Digenea: Monorchiidae), a parasite of Amiantis purpurata (Lamarck, 1818) (Bivalvia: Veneridae) and its corresponding metacercaria from the province of Buenos Aires and the Patagonian coast of the Southwest Atlantic Ocean, are described. The cercaria described in this paper differs from the three other monorchiid microcercous cercariae, i.e., Lasiotocus minutus (Manter, 1931), Lasiotocus elongatus (Manter, 1931), and Cercaria caribbea XXXVI Cable, 1956, mainly because of the extension of the excretory vesicle and the location of the ventral sucker. Cercariae artificially extracted from sporocysts...

D.W. Halton, J.M. Behnke, I. Marshall (Eds.): Practical Exercises in Parasitology.

Oleg Ditrich

Folia Parasitologica 48[3] 224 (2001) | DOI: 10.14411/fp.2001.036

Cambridge University Press, 2001. ISBN 0-521-79104-9, hardback, 461 pp., 2 halftones, 87 drawings, 87 diagrams, 4 tables. Price £30.00 (US$ 49.95).

Capillariid nematodes (Nematoda: Capillariidae) parasitic in the common cormorant (Phalacrocorax carbo), with redescription of Baruscapillaria carbonis (Dubinin et Dubinina, 1940)

Denisa Frantová

Folia Parasitologica 48[3] 225-230 (2001) | DOI: 10.14411/fp.2001.037

Two species of the genus Baruscapillaria Moravec, 1982 are known to parasitise the small intestine of the common cormorant, Phalacrocorax carbo (L.): Baruscapillaria carbonis (Dubinin et Dubinina, 1940) and B. rudolphii Moravec, Scholz et Našincová, 1994. A redescription of the former species, based on specimens collected from common cormorants shot in South Bohemia, Czech Republic, is provided. Morphological features distinguishing B. carbonis and B. rudolphii are specified. B. carbonis is characterised mainly by the well-developed membranous bursa in the male, composed of five distinct...

Egg shell ultrastructure of the fish nematode Huffmanela huffmani (Trichosomoididae)

Zdeňka Žďárská, David G. Huffman, František Moravec, Jana Nebesářová

Folia Parasitologica 48[3] 231-234 (2001) | DOI: 10.14411/fp.2001.038

The egg shell of Huffmanela huffmani Moravec, 1987 forms three main layers: an outer vitelline layer, a middle chitinous layer, and an inner lipid layer. The vitelline layer, forming the superficial projections of the egg shell, comprises two parts: an outer electron-dense, and an inner electron-lucid part. The chitinous layer is differentiated into three parts: an outer homogenous electron-dense part, a lamellated part, and an inner electron-dense net-like part. The lipid layer comprises an outer net-like electron-lucid part, and an inner homogenous electron-lucid part. The polar plugs are formed by electron-lucid material with fine electron-dense...

Comparison of virulence of Coxiella burnetii isolates from bovine milk and from ticks

Elena Kocianová, Elena Kováčová, Ivan Literák

Folia Parasitologica 48[3] 235-239 (2001) | DOI: 10.14411/fp.2001.039

Laboratory animals (mice and guinea pigs) were infected with the isolates of Coxiella burnetii (Derrick, 1939) obtained from bovine milk (M18 and M35) and the ticks Ixodes ricinus (Linnaeus, 1758) and Dermacentor marginatus (Sulzer, 1776) (Kl3 and Kl6, respectively), and with the reference strain Nine Mile. Neither mortality nor lethality occurred with the mice. Antibody response in mice infected with isolates from milk was lower (1 : 16-512) than that from ticks (1 : 32-4096). Onset of seropositivity also occurred later - on the 10th day post-infection (p.i.) for M18 and M35 in comparison with...

Lepeophtheirus simplex sp. n., a caligid copepod (Siphonostomatoida) parasitic on \"botete\" (bullseye puffer, Sphoeroides annulatus) in Sinaloa, Mexico

Ju-shey Ho, Samuel Gómez, Emma Fajer-Avila

Folia Parasitologica 48[3] 240-248 (2001) | DOI: 10.14411/fp.2001.040

A new species of caligid copepod, Lepeophtheirus simplex sp. n., parasitic on bullseye puffer, Sphoeroides annulatus (Jenyns) in Sinaloa, Mexico is described. The new species is distinguished from its congeners by the possession of (1) a maxillule with simple dentiform process; (2) a sternal furca with sharply pointed, curved tines; (3) a 2-segmented exopod of leg 3 with simple, slender spine on proximal segment; (4) a 3-segmented exopod of leg 4 with a long proximal, outer spine; and (5) the terminal claw of male antenna with a large, tridentate, medial protuberance.