Folia Parasitologica, vol. 53 (2006), issue 2

Virulent and attenuated lines of Leishmania major: DNA karyotypes and differences in metalloproteinase GP63

Jovana Sádlová, Petr Volf, Kathleen Victoir, Jean-Claude Dujardin, Jan Votýpka

Folia Parasitologica 53[2] 81-90 (2006) | DOI: 10.14411/fp.2006.011

The Leishmania metalloproteinase GP63 has been reported to play important roles mainly in resistance of promastigotes to complement-mediated lysis and in interaction with macrophage receptors. On the other hand, its function in insect vectors is still unclear. We compared the structure and dosage of gp63 genes and the activity of GP63 in Leishmania major Yakimoff et Schokhor strains and lines differing in virulence for mice and ability to develop in sand flies. The results demonstrate considerable variability in amount and proteolytical activity of GP63 among L. major strains although genomic changes in the gp63 locus...

New species of Choleoeimeria (Apicomplexa: Eimeriidae) from the veiled chameleon, Chamaeleo calyptratus (Sauria: Chamaeleonidae), with taxonomic revision of eimerian coccidia from chameleons

Michal Sloboda, David Modrý

Folia Parasitologica 53[2] 91-97 (2006) | DOI: 10.14411/fp.2006.012

Coprological examination of 71 samples from a breeding colony of veiled chameleons, Chamaeleo calyptratus Duméril et Duméril, 1851, revealed a presence of two species of coccidia. In 100% of the samples examined, oocysts of Isospora jaracimrmani Modrý et Koudela, 1995 were detected. A new coccidian species, Choleoeimeria hirbayah sp. n., was discovered in 32.4% of samples from the colony. Its oocysts are tetrasporocystic, cylindrical, 28.3 (25-30) × 14.8 (13.5-17.5) µm, with smooth, bilayered, ~1 µm thick wall. Sporocysts are dizoic, ovoidal to ellipsoidal, 10.1 (9-11) × 6.9 (6-7.5) µm, sporocyst wall is composed...

Temporal and spatial distribution of glochidial larval stages of European unionid mussels (Mollusca: Unionidae) on host fishes

Radim Blažek, Milan Gelnar

Folia Parasitologica 53[2] 98-106 (2006) | DOI: 10.14411/fp.2006.013

Glochidia are the larval stage of freshwater unionid mussels that parasitize the fins and gill apparatus of fish. A total of 22 fish species were examined for the presence of glochidia whose distribution on individual hosts was studied on three common fish species, the roach Rutilus rutilus (L.), perch Perca fluviatilis L. and bitterling Rhodeus sericeus (Pallas). Between 1997 and 1999, the fish were obtained from the rivers Morava and Kyjovka and surrounding water pools in the Czech Republic. The glochidia of two genera, Unio and Anodonta, were found. Anodonta glochidia were observed on 10 fish species, Unio...

Revision and phylogeny of Rhamnocercinae Monaco, Wood et Mizelle, 1954 (Monogenoidea: Diplectanidae)

Marcus V. Domingues, Walter A. Boeger

Folia Parasitologica 53[2] 107-116 (2006) | DOI: 10.14411/fp.2006.014

Species of Rhamnocercinae Monaco, Wood et Mizelle, 1954 are gill parasites of sciaenid fishes (Perciformes). Seven are marine species (three in the western Atlantic and four in oriental Pacific) and one is a neotropical freshwater species (Rio Doce Basin, Brazil). While the status of the subfamily may be questioned, this assemblage of species is apparently supported by several shared apomorphic and plesiomorphic characters, such as: (1) peduncular spines with anterior and posterior roots; (2) haptor laterally expanded, armed with anchors (two pairs); bars (one ventral, two dorsal); 14 hooks and haptoral accessory spines; and (3) double (nested) tubes...

Psettarium anthicum sp. n. (Digenea: Sanguinicolidae) from the heart of cobia Rachycentron canadum (Rachycentridae) in the northern Gulf of Mexico

Stephen A. Bullard, Robin M. Overstreet

Folia Parasitologica 53[2] 117-124 (2006) | DOI: 10.14411/fp.2006.015

Psettarium anthicum sp. n. (Digenea: Sanguinicolidae) infects the myocardium and atrial wall of the cobia Rachycentron canadum (Linnaeus, 1766) (Rachycentridae) in the northern Gulf of Mexico off Mississippi, USA. It is the first member of Psettarium Goto et Ozaki, 1930 reported from other than the Indian Ocean or Pacific Ocean and the second species of the genus reported from cobia. It differs from its congeners by the combination of having posterior caeca with lateral projections appearing as thorns in lateral view and the male pore anterior to the oötype. The species of Psettarium, P. japonicum (Goto et Ozaki,...

Ophiotaenia bonneti sp. n. (Eucestoda: Proteocephalidea), a parasite of Rana vaillanti (Anura: Ranidae) in Costa Rica

Alainde Chambrier, Sandrine C. Coquille, Daniel R. Brooks

Folia Parasitologica 53[2] 125-133 (2006) | DOI: 10.14411/fp.2006.016

Ophiotaenia bonneti sp. n. is described from the intestine of the frog Rana vaillanti Brocchi, 1877 (Anura: Ranidae) from San Gerardo, Guanacaste, Costa Rica. The new species is characterized by the testes 100-177 in number, the genital pores situated anteriorly, the osmoregulatory canals overlapping the testis field, the cirrus pouch length as 15-24% of proglottis width, and the uterus with 18-32 ramified diverticula on each side. It differs from the 23 known species of the genus Ophiotaenia La Rue, 1911, parasitic in amphibians, by one to several morphological characters. It differs from O. gracilis Jones, Cheng et Gillespie,...

Pseudodelphis limnicola sp. n. (Dracunculoidea: Guyanemidae) from the heart of Percichthys trucha (Perciformes: Percichthyidae) in Patagonian Andean lakes (Argentina)

Norma Brugni, Gustavo Viozzi

Folia Parasitologica 53[2] 134-138 (2006) | DOI: 10.14411/fp.2006.017

During a survey of parasites of Percichthys trucha (Cuvier et Valenciennes) (Perciformes: Percichthyidae) in northwestern Patagonian lakes, a new dracunculoid of the family Guyanemidae was found parasitizing as adults the heart of the fish. This species belongs to the genus Pseudodelphis Adamson et Roth, 1990 by having deirids, glandular multinucleate oesophagus and anterior branch of the uterus, and by lacking caudal alae and a gubernaculum. Pseudodelphis limnicola sp. n. differs from Pseudodelphis oligocotti Adamson et Roth, 1990 by the number and distribution of the cephalic papillae, a muscular oesophagus submerged into...

Two new species of philometrid nematodes (Nematoda: Philometridae) from the southern flounder Paralichthys lethostigma in the estuaries of South Carolina, USA

František Moravec, Isaurede Buron

Folia Parasitologica 53[2] 139-146 (2006) | DOI: 10.14411/fp.2006.018

Two new philometrid nematodes, Philometra overstreeti sp. n. and Philometroides paralichthydis sp. n., are described from female specimens collected from the southern flounder Paralichthys lethostigma Jordan et Gilbert (Paralichthyidae, Pleuronectiformes) in estuaries of South Carolina, USA. P. overstreeti is characterized by the presence of eight large, equal in size cephalic papillae, the absence of caudal projections, the body length of gravid females (14.10-27.06 mm) and the location in the host (among teeth). P. paralichthydis from the host's fins and the buccal cavity differs from its congeners mainly in possessing...

Relationships between the parasitic mite Spinturnix andegavinus (Acari: Spinturnicidae) and its bat host, Myotis daubentonii (Chiroptera: Vespertilionidae): seasonal, sex- and age-related variation in infestation and possible impact of the parasite on the host condition and roosting behaviour

Radek K. Lučan

Folia Parasitologica 53[2] 147-152 (2006) | DOI: 10.14411/fp.2006.019

Host-parasite relationships between the Daubenton's bat, Myotis daubentonii Kuhl, 1817 (Chiroptera: Vespertilionidae), and its haematophagous ectoparasite, the mite Spinturnix andegavinus Kolenati, 1857 (Acari: Spinturnicidae), were subjected to analyses based on data gathered during a six-year study (1999-2004) within a single study area in South Bohemia, Czech Republic. Seven hundred and fifty-one Daubenton's bats were examined by screening wing membranes with an intensive light source, resulting in 4,690 recorded mites. Sex, age, weight and reproductive state were evaluated for each bat. A body condition index was calculated as a ratio...

Effect of salivary gland extract from Ixodes ricinus ticks on the proliferation of Borrelia burgdorferi sensu stricto in vivo

Martina Macháčková, Miroslav Oborník, Jan Kopecký

Folia Parasitologica 53[2] 153-158 (2006) | DOI: 10.14411/fp.2006.020

Saliva-activated transmission (SAT) of Borrelia burgdorferi sensu stricto was demonstrated using real-time PCR and salivary gland extract (SGE) from partially fed Ixodes ricinus ticks. C3H/HeN mice were injected intradermally with 1.5 × 103 spirochetes mixed with 40 µg of SGE per mouse. The control group was inoculated with the same dose of spirochetes without SGE. The accelerating effect of SGE on spirochete proliferation was demonstrated on day 1 post infection, when a 4.2-fold increase in spirochetes was found in the skin and a 10-fold increase in the blood, compared with control mice. The data represent the first...

Rudolf W. Hoffmann: Fischkrankheiten.

Jiří Lom

Folia Parasitologica 53[2] 159 (2006) | DOI: 10.14411/fp.2006.021

Verlag Eugen Ulmer, Stuttgart, 2005. ISBN 3-8001-2739-3, hardback, 224 pp. Price not given.