Folia Parasitologica, vol. 60 (2013), issue 5

A drop in the bucket of the megadiverse chewing louse genus Myrsidea (Phthiraptera, Amblycera, Menoponidae): ten new species from Amazonian Brazil

Michel P. Valim, Jason D. Weckstein

Folia Parasitologica 60[5] 377-400 (2013) | DOI: 10.14411/fp.2013.040

Abstract: Ten new species of Myrsidea Waterston, 1915 parasitic on members of the avian families Formicariidae, Thraupidae, Tyrannidae, Troglodytidae and Icteridae are described herein. They and their type hosts are M. isacantha sp. n. ex Chamaeza nobilis Gould, M. circumsternata sp. n. ex Formicarius colma Boddaert (Formicariidae); M. cacioppoi sp. n. ex Lanio fulvus (Boddaert), Mbrasiliensis sp. n. ex Tangara chilensis (Vigors), M. saviti sp. n. ex Tangara schrankii (Spix) (Thraupidae), M. rodriguesae sp. n. ex Cnipodectes subbrunneus...

Nested PCR detection of Plasmodium malariae from microscopy confirmed P. falciparum samples in endemic area of NE India

Sunil Dhiman, Diganta Goswami, Dinesh Kumar, Bipul Rabha, Dhirendra Kumar Sharma, Rakesh Kumar Bhola, Indra Baruah, Vijay Veer

Folia Parasitologica 60[5] 401-405 (2013) | DOI: 10.14411/fp.2013.041

Abstract: The present study evaluates the performance of OptiMAL-IT® test and nested PCR assay in detection of malaria parasites. A total of 76 randomly selected blood samples collected from two malaria endemic areas were tested for malaria parasites using microscopy and OptiMAL-IT® test in the field. PCR assays were performed in the laboratory using DNA extracted from blood spots of the same samples collected on the FTA classic cards. Of the total of 61 field confirmed malaria positive samples, only 58 (95%) were detected positive using microscopy in the laboratory. Sensitivity, specificity, positive...

Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites

María de los Milagros Cámara, León A. Bouvier, Mariana R. Miranda, Chantal Reigada, Claudio A. Pereira

Folia Parasitologica 60[5] 406-410 (2013) | DOI: 10.14411/fp.2013.042

Abstract: Nowadays, there are no simple techniques for mimicking in vitro the life cycle of the kinetoplasmtid Trypanosoma cruzi Chagas, 1909, causative agent of Chagas disease, especially for parasite strains maintained as epimastigotes for many years. In the present study, we propose a method for obtaining metacyclic trypomastigotes, which were capable of infecting mammalian cells by simply lowering pH media. The collected amastigotes and trypomastigotes were differentiated into epimastigotes closing T. cruzi life cycle in vitro. Metacyclogenesis rates and infectivity were enhanced in cycled parasites. Finally, using this...

Molecular characteristics of the alpha- and beta-tubulin genes of Nosema philosamiae

Feng Zhu, Zhongyuan Shen, Li Xu, Xijie Guo

Folia Parasitologica 60[5] 411-415 (2013) | DOI: 10.14411/fp.2013.043

Abstract: Microsporidia are intracellular parasites of insects and other higher eukaryotes. The microsporidian Nosema philosamiae Talukdar, 1961 was isolated from the eri silkworm, Philosamia cynthia ricini Grote. In the present study, alpha- and beta-tubulin genes from N. philosamiae were characterized. The identity analysis of nucleotide and amino acid sequences indicated high similarity with species of Nosema Nägeli, 1857 sensu lato (nucleotide sequences, ≥ 96.0%; amino acid sequences, ≥ 99.0%). However, the tubulin genes of N. philosamiae share low sequence similarity with that of N. ceranae...

Differences in some developmental features between Toxoplasma gondii-seropositive and seronegative school children

Hanna Mizgajska-Wiktor, Wojciech Jarosz, Izabela Andrzejewska, Magdalena Krzyka³a, Jaros³aw Janowski, Maria Koz³owska

Folia Parasitologica 60[5] 416-424 (2013) | DOI: 10.14411/fp.2013.044

Abstract: Knowledge about the influence of latent toxoplasmosis on development and general biological condition of children is scant and thus the aim of the present study was to investigate these aspects in some detail. We compare school children in rural area seropositive and seronegative to the apicomplexan parasite Toxoplasma gondii (Nicolle et Manceaux, 1908) in terms of their developmental age, body mass and body height, physical fitness and end-of-term grades. Additionally, we evaluated the risk factors of infection with T. gondii such as the presence of cats in the household and eating raw meat products. With IFAT and ELISA tests,...

Sphaeromyxa artedielli sp. n. (Myxozoa: Sphaeromyxidae), a parasite of sculpins (Cottidae) in northern Norway

Egil Karlsbakk, Ann-Cathrine Bårdsgjære Einen, Pavla Barto¹ová

Folia Parasitologica 60[5] 425-432 (2013) | DOI: 10.14411/fp.2013.045

Abstract: Sphaeromyxa artedielli sp. n. is described from the gall bladder of the Atlantic hookear sculpin Artediellus atlanticus Jordan et Evermann (Cottidae; type host) from northern Norway. The parasite was also found to infect Triglops murrayi Günther (Cottidae). Spores are produced in disporic pansporoblasts in large flat plasmodia. Spores are straight and fusiform with truncated ends, and measure 16.5-18.7 µm × 4.9-6.2 µm. Valves are thick, striated and suture line is straight. Two equal ovoid polar capsules measure 4.2-6.8 µm × 2.9-4.4 µm and contain irregularly folded polar filaments. Distinctive...

Ligophorus species (Monogenea: Ancyrocephalidae) from Mugil cephalus (Teleostei: Mugilidae) off Morocco with the description of a new species and remarks about the use of Ligophorus spp. as biological markers of host populations

Fouzia El Hafidi, Ouafae Berrada Rkhami, Isaure de Buron, Jean-Dominique Durand, Antoine Pariselle

Folia Parasitologica 60[5] 433-440 (2013) | DOI: 10.14411/fp.2013.046

Abstract: Gill monogenean species of Ligophorus Euzet et Suriano, 1977 were studied from the teleost Mugil cephalus Linneaus (Mugilidae) from the Mediterranean and Atlantic coasts of Morocco. We report the presence of L. mediterraneus from both the Mediterranean and Atlantic coast and L. cephali and L. maroccanus sp. n. from the Atlantic coast only. The latter species, which is described herein as new, resembles L. guanduensis but differs from this species mainly in having a shorter penis compared to the accessory piece, a proportionally longer extremity of the accessory piece and a less developed heel....

Gyrodactylus aff. mugili Zhukov, 1970 (Monogenoidea: Gyro-dactylidae) from the gills of mullets (Mugiliformes: Mugilidae) collected from the inland waters of southern Iraq, with an evalutation of previous records of Gyrodactylus spp. on mullets in Iraq

Delane C. Kritsky, Atheer H. Ali, Najim R. Khamees

Folia Parasitologica 60[5] 441-447 (2013) | DOI: 10.14411/fp.2013.047

Abstract: Gyrodactylus aff. mugili Zhukov, 1970 (Monogenoidea: Gyrodactylidae) is recorded and described from the gill lamellae of 11 of 35 greenback mullet, Chelon subviridis (Valenciennes) (minimum prevalence 31%), from the brackish waters of the Shatt Al-Arab Estuary in southern Iraq. The gyrodactylid was also found on the gill lamellae of one of eight Speigler's mullet, Valamugil speigleri (Bleeker), from the brackish waters of the Shatt Al-Basrah Canal (minimum prevalence 13%). Fifteen Klunzinger's mullet, Liza klunzingeri (Day), and 13 keeled mullet, Liza carinata (Valenciennes), collected and examined...

Two new species of Acanthobothrium (Tetraphyllidea: Onchobothriidae) from Pastinachus cf. sephen (Myliobatiformes: Dasyatidae) from the Persian Gulf and Gulf of Oman

Loghman Maleki, Masoumeh Malek, Harry W. Palm

Folia Parasitologica 60[5] 448-456 (2013) | DOI: 10.14411/fp.2013.048

Abstract: Two new species of Acanthobothrium van Beneden, 1850 from the spiral intestine of Pastinachus cf. sephen Forsskål from the Iranian coast of the Persian Gulf and the Gulf of Oman are described. To analyse the surface ultrastructure the worms were studied using light and scanning electron microscopy. Acanthobothrium jalalii sp. n. belongs to the category 1 species of the genus so far including 43 species. This tiny new species differs from the other category 1 species by its small total length (2.18 ± 0.49 mm), number of proglottids (4.7 ± 0.9) and testes (24 ± 3), terminal segments...

Infective pentastomid larvae from Pygocentrus nattereri Kner (Pisces, Characidae) from the Miranda River, Pantanal, Mato Grosso do Sul State, Brazil, with notes on their taxonomy and epidemiology

Suely C. Giesen, Ricardo M. Takemoto, Frikkie Calitz, Maria de los Angeles Perez Lizama, Kerstin Junker

Folia Parasitologica 60[5] 457-468 (2013) | DOI: 10.14411/fp.2013.049

Abstract: During parasitological surveys of freshwater fish from the Miranda River, Brazil, 199 Pygocentrus nattereri kner (Characidae) were caught. Two pentastomid families, Subtriquetridae Fain, 1961, represented by its single genus Subtriquetra Sambon, 1922, and Sebekidae Sambon, 1922, represented by three genera, were present. Free-living larvae of Subtriquetra subtriquetra (Diesing, 1835) were collected from the swim bladder. Encysted larvae of Alofia Giglioli, 1922 were found in the abdominal cavity, chambers of the heart, musculature, on the surface of the gonads and swim bladder. Some Alofia larvae were moving...

A new species of Rhabdias Stiles et Hassall, 1905 (Nematoda: Rhabdiasidae) from Blommersia domerguei (Guibé) (Amphibia: Mantellidae) in Madagascar

Yuriy Kuzmin, Kerstin Junker, Louis du Preez, Odile Bain

Folia Parasitologica 60[5] 469-474 (2013) | DOI: 10.14411/fp.2013.050

Abstract: Rhabdias blommersiae sp. n. (Nematoda: Rhabdiasidae) is described from the lungs of Domergue's Madagascar frog, Blommersia domerguei (Guibé) (Amphibia: Mantellidae), in Madagascar. The new species differs from congeners parasitizing amphibians in having a smaller body and buccal capsule, six equal lips, large excretory glands of unequal length and a posteriorly inflated body vesicle. A combination of characters distinguishes it from Afromalagasy species of Rhabdias Stiles et Hassall, 1905. Rhabdias blommersiae is the third species of the genus described from amphibians in Madagascar. Close similarities in the number...

Afrojoyeuxia gen. n. and Hunkeleriella gen. n., two new genera of cestodes (Cyclophyllidea: Anoplocephalidae) from African rodents

Voitto Haukisalmi

Folia Parasitologica 60[5] 475-481 (2013) | DOI: 10.14411/fp.2013.051

Abstract: Based on the study of type material, two new genera of cestodes (Cyclophyllidea: Anoplocephalidae) are proposed for Paranoplocephala Lühe, 1910 sensu lato species from African rodents. Afrojoyeuxia gen. n., proposed for A. gundii (Joyeux, 1923) comb. n. from Ctenodactylus gundi (Rothmann) (Hystricomorpha: Ctenodactylidae), is characterized by a high length/width ratio of mature proglottids, longitudinally extensive testicular field positioned anterior to the female glands, an ovoid or subspherical cirrus-sac and a thick, conical cirrus. Hunkeleriella gen. n., proposed for H. dasymidis (Hunkeler,...