Folia Parasitologica, vol. 64 (2017)

Folia Parasitologica 64:022 (2017) | DOI: 10.14411/fp.2017.022

Musculoskeletal and nervous systems of the attachment organ in three species of Diplectanum (Monogenea: Dactylogyroidea)

Anatoly A. Petrov1, Evgenija V. Dmitrieva2, Maryana P. Popyuk3, Pavel I. Gerasev1, Sergey A. Petrov1
1 Zoological Institute of the Russian Academy of Sciences, Saint Petersburg, Russia;
2 A.O. Kovalevsky Institute of Marine Biological Research, Sevastopol, Crimea;
3 Murmansk Marine Biological Institute, Murmansk, Russia

The taxonomy of Diplectanum Diesing, 1858, a genus of monopisthocotylean monogeneans, remains unsettled and needs to be revised based on new morphological criteria. Recent studies in monopisthocotyleans have shown that the muscle arrangement in the posterior attachment organ (haptor) differs between congeneric species and can be used as an additional criterion in genus-level taxonomy. To explore the possibility of using the haptoral musculature and nervous system in the taxonomy of Diplectanum, we conducted a detailed confocal-microscopy study of three species of Diplectanum (D. aculeatum Parona et Perugia, 1889, D. sciaenae van Beneden et Hesse, 1863 and D. similis Bychowsky, 1957) with phalloidin staining for muscle and indirect immunostaining for 5HT and FMRFamide. A further goal was to clarify the functional mechanics of the haptor and the role of its essential components (squamodiscs and anchors) in attachment to the host. The system of connecting bars and gaffing anchors was found to have a complex musculature consisting of 23 muscles in D. aculeatum and D. sciaenae, and 21 muscles in D. similis. The squamodiscs were shown to be operated by several groups of muscles attached primarily to the area termed the squamodisc fulcrum. Most of the haptoral musculature is identical in D. aculeatum and D. sciaenae and these species differ only in the presence of a muscle sheath around the tissue strand between the squamodiscs in D. sciaenae and in the different patterns of superficial squamodisc muscles. Diplectanum similis shows more significant differences from the other two species: besides lacking two of the haptoral muscles, it also differs in the shapes and arrangement of several other muscles. The nervous system of all three species conforms to the general pattern typical for the Dactylogyroidea and shows little variation between species.

Keywords: Monopisthocotylea, musculature, haptor, phalloidin, 5HT, FMRFamide, squamodisc, anchor

Received: April 4, 2017; Accepted: June 13, 2017; Published online: July 11, 2017


References

  1. Buchmann K., Prento P. 1989: Cholinergic and aminergic elements in the nervous system of Pseudodactylogyrus bini (Monogenea). Dis. Aquat. Organ. 6: 89-92. Go to original source...
  2. Dezfuli B.S., Giari L., Simoni E., Menegatti R., Shinn A.P., Manera M. 2007: Gill histopathology of cultured European sea bass, Dicentrarchus labrax (L.), infected with Diplectanum aequans (Wagener 1857) Diesing 1958 (Diplectanidae: Monogenea). Parasitol. Res. 100: 707-713. Go to original source... Go to PubMed...
  3. Domingues M.V., Boeger W.A. 2008: Phylogeny and revision of Diplectanidae Monticelli, 1903 (Platyhelminthes: Monogenoidea). Zootaxa 1698: 1-40.
  4. Galli P., Pugachev O.N., Kritsky D. (Eds.) 2009: Guide to Monogenoidea of Freshwater Fish of Palaeartic and Amur Regions. Ledizione-Ledi Publishing, Milan, 564 pp.
  5. Gerasev P.I. 1977: [The mechanism of attachment to the host's gills in Dactylogyrus extensus and D. achmerowi (Monogenoidea)]. Parazitologiya 11: 513-519. (In Russian.)
  6. Gerasev P.I. 1981: [Muscle systems of haptors and the attachment mechanism of some species of Dactylogyrus (Monogenea, Dactylogyridae)]. Parazitol. Sb. 30: 190-205. (In Russian.)
  7. Gerasev P.I. 1989: [Major muscles of anchors in Amuro-Chinese species of Dactylogyrus (Monogenoidea)]. In: B.I. Lebedev (Ed.), Parazitologicheskiye Issledovaniya. DVO AN SSSR, Vladivostok, pp. 39-45 (In Russian.)
  8. Gibson D.I., Bray R.A. 2010: Diplectanum Diesing, 1858. In: S. Tyler, T. Artois, S. Schilling, M. Hooge and L.F. Bush (Eds.), World List of Platyhelminthes. World Wide Web electronic publication, http://www.marinespecies.org/aphia.php?p=taxdetails&id=119291.
  9. Justine J.-L., Henry É. 2010: Monogeneans from Epinephelus chlorostigma (Val.) (Perciformes: Serranidae) off New Caledonia, with the description of three new species of diplectanids. Syst. Parasitol. 77: 81-105. Go to original source... Go to PubMed...
  10. Kearn G.C. 1971: The attachment of the ancyrocephalid monogenean Haliotrema balisticus to the gills of the trigger fish, Balistes capriscus (= carolinensis). Parasitology 63: 157-162. Go to original source... Go to PubMed...
  11. Kearn G.C., Beverley-Burton M. 1990. Mycteronastes undulatae gen. nov., sp. nov. (Monogenea: Monocotylidae) from the nasal cavities of Raja undulata in the Eastern Atlantic. J. Mar. Biol. Ass. UK 70: 747-753. Go to original source...
  12. Kearn G.C., Whittington I., Evans-Gowing R. 2003. Striated (stratified) muscle and the operation of the ventral hamuli of the ancryocephaline monogenean Chauhanellus australis, from the gills of the blue catfish Arius graeffei. In: C. Combes and J. Jourdane (Eds.), Taxonomie, Écologie et Évolution des Métazoaires Parasites. Tome I. Presses Universitaires de Perpignan, Perpignan, pp. 381-396.
  13. Oliver G. 1968: Recherches sur les Diplectanidae (Monogenea) parasites de Téléostéens du Golfe du Lion. I. Diplectaninae Monticelli 1903. Vie Milieu 19: 95-138.
  14. Oliver G. 1969: Recherches sur les Diplectanidae (Monogenea) parasites de Téléostéens du Golfe du Lyon. III. Biology. Vie Milieu 20: 397-419.
  15. Oliver G. 1980: Les Diplectanidae Bychowsky, 1957 (Monogenea, Monopisthocotylea) parasites des Sciaenidae (Pisces, Perciformes) du Golfe de Gascogne. Bull. Mus. Natl. Hist. Nat. 23: 669-689.
  16. Oliver G. 1987: Les Diplectanidae Bychowsky, 1957 (Monogenea: Monopisthocotylea, Dactylogyridea). Systematique. Biologie. Ontogénie. Ecologie. Essai de phylogenèse. Academie de Montpellier, Université des Sciences et Techniques du Languedoc, Montpellier, 443 pp.
  17. Oliver G., Paperna I. 1984: Diplectanidae Bychowsky, 1957 (Monogenea, Monopisthocotylea) parasites de Perciformes de Méditerranée orientale, de la Mer Rouge et de l'Océan Indien. B. Mus. Natl. Hist. Nat. 6: 49-65.
  18. Paling J.E. 1966: The attachment of the monogenean Diplectanum aequans (Wagener) Diesing to the gills of Morone labrax L. Parasitology 56: 493-593. Go to original source... Go to PubMed...
  19. Petrov A.A., Popyuk M.P., Dmitrieva E.V., Gerasev P.I. 2015: Architecture of haptoral musculature in three species of Ligophorus (Monogenea, Ancyrocephalidae). Trudy Zool. Inst. RAN 319: 244-256.
  20. Petrov A.A., Popyuk M.P., Gerasev P.I., Dmitrieva E.V. 2016: Haptoral neuromusculature in two species of Dactylogyrus Diesing, 1850 (Monogenea: Dactylogyridae). Syst. Parasitol. 93: 337-354. Go to original source... Go to PubMed...
  21. Sánchez-García N., Padrós F., Raga J.A., Montero F.E. 2011: Comparative study of the three attachment mechanisms of diplectanid monogeneans. Aquaculture 318: 290-299. Go to original source...
  22. Schmidt-Rhaesa A. 2007. The Evolution of Organ Systems. Oxford University Press: Oxford, New York, 385 pp.
  23. Shaw M.K. 1981: The ultrastructure of the pseudohaptoral squamodiscus of Diplectanum aequans (Monogenea). Parasitology 82: 231-240. Go to original source... Go to PubMed...
  24. Timofeeva T.A., Kotikova E.A. 1993: [Nervous system of the monogeneans of the orders Tetraonchoidea and Dactylogyroidea (Monogenea)]. Parazitologiya 27: 118-126. (In Russian.)
  25. Timofeeva T.A., Kotikova E.A. 1995: [Topographical peculiarities of monogenean nervous systems]. Parazitologiya 29: 127-139. (In Russian.)