Folia Parasitologica 47[1] 49-54 (2000) | DOI: 10.14411/fp.2000.009

A study of proteases throughout the life cycle of Trichinella spiralis

Rosa Mª Ros-Moreno, Celia Vázquez-López, Consuelo Giménez-Pardo, Cristina de Armas-Serra, Filomena Rodríguez-Caabeiro
Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Alcalá de Henares 28871, Madrid, Spain

In the present report we study the proteolytic activity of the excretion-secretion and crude extracts of different stages of Trichinella spiralis (Owen, 1835) Railliet, 1895, (muscle-stage larvae, adult worms before and after mating, and newborn larvae) using natural substrates (structural and hematic mammalian proteins). The analysis of the results allow us to set up a certain stage-specificity, as well as an important relationship between the protease patterns throughout the parasite life cycle and how the parasite may overcome both mechanical and humoral barriers within the host. Muscle-stage larvae present a great activity against structural proteins (collagen), while newborn larvae and adult worms degrade principally hematic proteins (hemoglobin, fibrinogen and immunoglobulin G).

Keywords: Trichinella spiralis, proteases, life cycle

Received: April 23, 1999; Accepted: September 15, 1999; Published: March 1, 2000  Show citation

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Ros-Moreno, R.M., Vázquez-López, C., Giménez-Pardo, C., de Armas-Serra, C., & Rodríguez-Caabeiro, F. (2000). A study of proteases throughout the life cycle of Trichinella spiralis. Folia Parasitologica, 47(1), 49-54. doi: 10.14411/fp.2000.009
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