Folia Parasitologica, vol. 66 (2019)
New records of philometrids (Nematoda: Philometridae) from marine fishes off Japan, including description of Philometra kidakoi sp. n. and Congerinema japonicum gen. et sp. n.
Franti¹ek Moravec, Kazuya Nagasawa, Masato Nitta, Atsushi Tawa
Folia Parasitologica 66:021 (2019) | DOI: 10.14411/fp.2019.021 
Occasional examinations of some marine fishes in Japan revealed, in addition to other parasites, the following eight species of philometrid nematodes (Philometridae) (all females only): Philometra kidakoi sp. n. from the ovary of Gymnothorax kidako (Temminck et Schlegel) (Muraenidae); Philometra pinnicola (Yamaguti, 1935) from the operculum of Epinephelus akaara (Temminck et Schlegel) (Serranidae); Philometra sebastisci Yamaguti, 1941 from the ovary of Sebastes cheni Barsukov (Scorpaenidae) (new host); Philometra madai Quiazon, Yoshinaga et Ogawa, 2008 from the ovary of Acanthopagrus schlegelii...
The molecular detection of Anaplasma phagocytophilum and Rickettsia spp. in cat and dog fleas collected from companion animals
Olga Pawe³czyk, Marek Asman, Krzysztof Solarz
Folia Parasitologica 66:020 (2019) | DOI: 10.14411/fp.2019.020
Companion animals can be infested by various species of parasitic insects. Cat flea Ctenocephalides felis (C. felis felis) (Bouché, 1835) and dog flea Ctenocephalides canis (Curtis, 1826) belong to multihost external parasites of mammals, which most frequently occur on domestic cats Felis catus Linnaeus and dogs Canis familiaris Linnaeus. The main aim of this study was to investigate the presence of pathogens, such as Anaplasma phagocytophilum (syn. Ehrlichia phagocytophila) and Rickettsia spp., in adult C. felis and C. canis fleas. Flea sampling has been realised from January 2013 to April...
Low seroprevalence and low incidence of infection with Toxoplasma gondii (Nicolle et Manceaux, 1908) in pediatric hematopoietic cell transplantation donors and recipients: Polish nationwide study
Krzysztof Czyzewski, Jowita Fraczkiewicz, Malgorzata Salamonowicz, Anna Pieczonka, Olga Zajac-Spychala, Agnieszka Zaucha-Prazmo, Jolanta Gozdzik, Jan Styczynski
Folia Parasitologica 66:019 (2019) | DOI: 10.14411/fp.2019.019
Toxoplasmosis is a potentially fatal complication after hematopoietic cell transplantation (HCT). Pre-transplant seropositivity of graft recipient to Toxoplasma gondii (Nicolle et Manceaux, 1908) is an important factor for disease reactivation after HCT. As toxoplasmosis epidemiology varies all over the world, we performed a Polish nationwide retrospective cohort study to determine the seroprevalence of toxoplasmosis in donors and pediatric allogeneic and autologous HCT recipients and the incidence of clinically evident toxoplasmosis in this patient group. Polish adult donors had higher anti-T. gondii seroprevalence than Polish pediatric...
Alpine bullhead (Cottus poecilopus Heckel): a potential refuge for Gyrodactylus salaris Malmberg, 1957 (Monogenea)
Tor A. Bakke, Rachel A. Paterson, Jo Cable
Folia Parasitologica 66:018 (2019) | DOI: 10.14411/fp.2019.018
The notifiable freshwater pathogen Gyrodactylus salaris Malmberg, 1957 tends to be a generalist in contrast to other monogeneans. Whilst it causes most damage to its primary host, the Atlantic salmon (Salmo salar Linnaeus), transport and reservoir hosts likely play a key role in maintaining the parasite in the environment. Here, we tested the ability of G. salaris (strain River Lierelva, southern Norway) to infect and reproduce on a population of wild caught alpine bullhead (Cottus poecilopus Heckel). Exposure of alpine bullhead yearlings (0+) to G. salaris for 24 h at low (6.5 °C) or high temperature (11.5 °C) resulted...
Bat-infesting chiggers (Acariformes: Trombiculidae) of the Balearic Islands and new data on the genus Trisetica Traub et Evans, 1950
Alexandr A. Stekolnikov, Juan Quetglas
Folia Parasitologica 66:017 (2019) | DOI: 10.14411/fp.2019.017
A sample of chigger mites from bat hosts collected in the Balearic Islands (Western Mediterranean Sea) is found to include two species. These are the first records of bat-infesting chiggers identified to species in Spain. Chiggers collected from Pipistrellus kuhlii (Kuhl) in Menorca are identified as Oudemansidium komareki (Daniel et Dusbábek, 1959); this species, which was known from Austria, Bulgaria, Romania, Slovakia, Moldova, Crimea, and Azerbaijan, is recorded for the first time in Spain. Chiggers collected from Plecotus austriacus (Fischer) in Formentera are identified as Trombicula knighti Radford, 1954, which was...
Measuring mitochondrial respiration in adherent cells infected with Trypanosoma cruzi Chagas, 1909 using Seahorse extracellular flux analyser
Laura Maria González-Ortiz*, Juana Patricia Sánchez-Villamil*, Mike A. Celis-Rodríguez, Giovanni Lineros, Sandra Sanabria-Barrera, Norma C. Serrano, Melvin Y. Rincon, Paula K. Bautista-Nino
Folia Parasitologica 66:016 (2019) | DOI: 10.14411/fp.2019.016
Infection with Trypanosoma cruzi Chagas, 1909 is reported to increase the production of reactive oxygen species in patients with Chagas disease. Mitochondria dysfunction, host inflammatory response and inadequate antioxidant response are described as the main factors leading to oxidative stress during acute and chronic stages of the disease. The Seahorse XFe24 extracellular flux platform allows energy metabolism determination through mitochondrial respiration and glycolysis measurements. XFe24 platform can be used in in vitro models of T. cruzi-infected cells, which allow the assessment and even modulation of endogenous conditions...
Description of Acanthocephalus anguillae balkanicus subsp. n. (Acanthocephala: Echinorhynchidae) from Proteus anguinus Laurenti (Amphibia: Proteidae) and the cave ecomorph of Asellus aquaticus (Crustacea: Asellidae) in Slovenia
Omar M. Amin, Richard A. Heckmann, ®iga Fi¹er, Valerija Zak¹ek, Holger Herlyn, Rok Kostanj¹ek
Folia Parasitologica 66:015 (2019) | DOI: 10.14411/fp.2019.015
Acanthocephalus balkanicus Batchvarov et Combes, 1974 was incompletely described from the northern crested newt, Triturus cristatus (Laurenti) (Amphibia: Salamandridae), a possible synonym of the Balkan crested newt, Triturus ivanbureschi Arntzen et Wielstra, from a pond in village of Pesnopoy, southern Bulgaria. We provide a full description of adult males and females of the same taxon from the olm, Proteus anguinus Laurenti (Amphibia: Proteidae), the only exclusively aquatic cave-dwelling vertebrate in Europe, captured in Postojna-Planina Cave System in Slovenia. Cystacanths were also collected from the cave ecomorph of Asellus...
Redescription and neotype designation for the poorly known fish parasitic cymothoid Joryma brachysoma (Pillai, 1964) (Crustacea: Isopoda) from India
Panakkool Thamban Aneesh, Ameri Kottarathil Helna, Appukuttannair Biju Kumar
Folia Parasitologica 66:014 (2019) | DOI: 10.14411/fp.2019.014
A neotype is designated for Joryma brachysoma (Pillai, 1964). The general morphology and appendages of the female and male stages are illustrated and re-described based on the neotype and several additional fresh specimens collected from the type host Pellona brachysoma Bleeker (=Sardinella brachysoma Bleeker) from nearby the type locality. The present redescription and neotype designation further resolves the taxonomic ambiguity regarding the species identification and conserves the name of J. brachysoma.
Toxoplasma gondii RPL40 is a circulating antigen with immune protection effect
Junxin Xue, Wei Jiang, Jian Li, Wei Xiong, Zhengan Tian, Qiang Zhang, Shuqing Li, Chunfeng Liu, Kehe Huang, Quan Wang
Folia Parasitologica 66:013 (2019) | DOI: 10.14411/fp.2019.013
Screening and identification of protective antigens are essential for the prevention of infections with Toxoplasma gondii (Nicolle et Manceaux, 1908). In our previous study, T. gondii ribosomal-ubiquitin protein L40 (TgRPL40) was identified as a circulating antigen. However, the function and protective value of TgRPL40 was unknown. In the current study, recombinant TgRPL40 was expressed in Escherichia coli BL21 and antibody was prepared. Western blotting analysis indicated that TgRPL40 was present in circulating antigens and excretory/secretary antigens (ESA). Immunofluorescence and immunoelectron microscopy analysis...
Three new species of acanthocephalans (Palaeacanthocephala) from marine fishes collected off the East Coast of South Africa
Olga I. Lisitsyna, Olena Kudlai, Thomas H. Cribb, Nico J. Smit
Folia Parasitologica 66:012 (2019) | DOI: 10.14411/fp.2019.012
Three new species of acanthocephalans are described from marine fishes collected in Sodwana Bay, South Africa: Rhadinorhynchus gerberi n. sp. from Trachinotus botla (Shaw), Pararhadinorhynchus sodwanensis n. sp. from Pomadasys furcatus (Bloch et Schneider) and Transvena pichelinae n. sp. from Thalassoma purpureum (Forsskål). Transvena pichelinae n. sp. differs from the single existing species of the genus Transvena annulospinosa Pichelin et Cribb, 2001, by the lower number of longitudinal rows of hooks (10-12 vs 12-14, respectively) and fewer hooks in a row (5 vs 6-8),...
First molecular assessment of the interrelationships of cladorchiid digeneans (Digenea: Paramphistomoidea), parasites of Neotropical fishes, including descriptions of three new species and new host and geographical records
Camila Pantoja, Tomá¹ Scholz, José Luis Luque, Arlene Jones
Folia Parasitologica 66:011 (2019) | DOI: 10.14411/fp.2019.011
The first molecular assessment of phylogenetic relationships of cladorchiid digeneans (superfamily Paramphistomoidea Fischoeder, 1901) from freshwater fishes based on 28S rDNA, ITS2 and cox1 sequences reveals the subfamilies Dadayiinae Fukui, 1929 and Kalitrematinae Travassos, 1933 as non-monophyletic, whereas Dadaytrema Travassos, 1931 represented by three species is monophyletic. Fourteen species of cladorchiids were found in characiform, perciform and siluriform fishes in the Neotropical Region (Brazil and Peru), with numerous new host and geographical records. The first scanning electron micrographs of seven species are presented....
Morphological and molecular characteristics of adult worms of Gnathostoma Owen, 1836 (Nematoda) collected from domestic pigs in Dien Bien Province, northern Vietnam
Nguyen Van Tuyen, Nguyen Thi Kim Lan, Pham Ngoc Doanh
Folia Parasitologica 66:010 (2019) | DOI: 10.14411/fp.2019.010
Gnathostomes are of interest because of their unique appearance and medical importance. Among 13 valid species of the genus Gnathostoma Owen, 1836 (Nematoda: Spirurida), two species, G. doloresi Tubangui, 1925 and G. hispidum Fedtschenko, 1872, are parasites of pigs but their larvae can infect humans to cause gnathostomiasis. In this study, we collected adults of Gnathostoma sp. from the stomach of domestic pigs (Sus scrofa domesticus Linnaeus) from Dien Bien Province, northern Vietnam. Morphologically, nematodes found here are most similar to G. doloresi with a slight difference in the spicules...
Insight into the species diversity of the quill mite genus Betasyringophiloidus Skoracki, 2011 (Prostigmata: Syringophilidae) on the basis of the DNA barcodes
Eliza Glowska, Lukasz Broda, Miroslawa Dabert
Folia Parasitologica 66:009 (2019) | DOI: 10.14411/fp.2019.009
Betasyringophiloidus Skoracki, 2011 is a genus of quill mites (Prostigmata: Syringophilidae) that is believed to contain mono-, steno- and polyxenous parasites associated with a wide range of passerine birds (Passeriformes) across the world. In this work we applied the DNA-barcode marker (mitochondrial cytochrome c oxidase subunit I gene fragment, COI) to verify whether Betasyringophiloidus schoeniclus (Skoracki, 2002) and Betasyringophiloidus seiuri (Clark, 1964) are actual steno- and polyxenous species associated with the currently recognised host ranges, or their populations are highly host-specific, cryptic species. Our results...
First evidence of serotoninergic components in the nervous system of the monogenean Chimaericola leptogaster (Chimaericolidae, Polyopisthocotylea), a gill parasite of the relict holocephalan fish
Natalya V. Mochalova, Nadezhda B. Terenina, Larisa G. Poddubnaya, Valery A. Yashin, Andrei V. Kuchin, Natalya D. Kreshchenko
Folia Parasitologica 66:008 (2019) | DOI: 10.14411/fp.2019.008
The localisation and distribution of the serotoninergic nerve elements was studied for the first time in the flatworm Chimaericola leptogaster (Leuckart, 1830) using immunocytochemical methodology and confocal laser scanning microscopy. The musculature was investigated by histochemical staining of actin filaments; scanning electron microscopy was used to identify the sensory structures on the worm's surface. Uniciliated, bi-ciliated and multiciliated sensory endings have been described on the worm's surface. The morphological data demonstrate the presence of circular, longitudinal and diagonal muscles that comprise the musculature of C. leptogaster...
Rhipicephalus sanguineus sensu lato from dogs and dromedary camels in Riyadh, Saudi Arabia: low prevalence of vector-borne pathogens in dogs detected using multiplexed tandem PCR panel
Shona Chandra, Karen Smith, Abdullah D. Alanazi, Mohamed S. Alyousif, David Emery, Jan ©lapeta
Folia Parasitologica 66:007 (2019) | DOI: 10.14411/fp.2019.007
Despite the global distribution of the brown dog tick, Rhipicephalus sanguineus (Latreille, 1806) sensu lato (s.l.), limited information exists about their identity from the Arabian Peninsula. Ticks from free roaming urban dogs and dromedary camels in Riyadh, Saudi Arabia were morphologically identified, confirmed with scanning electron microscopy and characterised at mitochondrial DNA (cox1, 12S rDNA and 16S rDNA). A total of 186 ticks were collected from 65 free roaming dogs (n = 73) and 84 dromedary camels (n = 113). Morphologically, 5.9% (11/186) were R. sanguineus s.l. and Hyalomma spp. (93.5%,...
K13 propeller domain mutations and pfmdr1 amplification in isolates of Plasmodium falciparum collected from Thai-Myanmar border area in 2006–2010
Papichaya Phompradit, Wanna Chaijaroenkul, Phunuch Muhamad, Kesara Na-Bangchang
Folia Parasitologica 66:006 (2019) | DOI: 10.14411/fp.2019.006
The K13 propeller domain mutation and pfmdr1 amplification have been proposed as useful molecular markers for detection and monitoring of artemisinin resistant Plasmodium falciparum Welch, 1897. Genomic DNA isolates of P. falciparum was extracted from 235 dried blood spot or whole blood samples collected from patients with uncomplicated falciparum malaria residing in areas along the Thai-Myanmar border during 2006-2010. Nested polymerase chain reaction (PCR) and sequencing were performed to detect mutations in K13 propeller domain of P. falciparum at codon 427-709....
Preparation of monoclonal antibodies against Bc48 and development of a rapid detection assay for infection with Babesia caballi in China
Panju Wang, Jingjing Song, Ruiqi Song, Mengyuan Zhang, Lijiang Wu, Fangxin Li, Yan Yan, Jiyong Zhou, Bayin Chahan, Min Liao
Folia Parasitologica 66:005 (2019) | DOI: 10.14411/fp.2019.005
Babesia caballi (Nuttal, 1910) is one of the causative agents of equine piroplasmosis which causes economic losses to horse industry in China. There is an urgent need for rapid detection method for B. caballi infection in Xinjiang Province, China. To prepare monoclonal antibodies (mAbs) against Bc48 gene of B. caballi (Xinjiang local strains) and establish colloidal gold-immunochromatographic (ICT) assay for diagnosis of the disease, recombinant Bc48 was expressed and purified from Escherichia coli. With purified Bc48 as immunogen in mice, three hybridoma cells named 11F4, 1H2 and 7F4 secreting mAbs against Bc48 of B....
A new genus and four new species of dactylogyrids (Monogenea), gill parasites of pimelodid catfishes (Siluriformes: Pimelodidae) in South America and the reassignment of Urocleidoides megorchis Mizelle et Kritsky, 1969
Aline A. Acosta, Carlos A. Mendoza-Palmero, Reinaldo J. da Silva, Tomá¹ Scholz
Folia Parasitologica 66:004 (2019) | DOI: 10.14411/fp.2019.004
Nanayella gen. n. is proposed to accommodate four new species of dactylogyrid monogeneans found on the gills of two species of pimelodid catfishes (Siluriformes) from the Amazon River basin in Peru and the Upper Paraná River basin in Brazil. Nanayella gen. n. is mainly characterised by the presence of modified hook pairs 5 and 6, which have a slender shank with slightly enlarged base, inconspicuous thumb, and straight point. Nanayella aculeatrium gen. n. et sp. n. (type species) from Sorubim lima (Bloch et Schneider) (type host) differs from its congeners mainly by possessing seven sclerotised spines on one side of circular ornamentation...
A description of the male of Geckobiella donnae Paredes-León, Klompen et Pérez, 2012 (Acari: Pterygosomatidae) from captive iguanas in Honduras
Stanislav Kalúz, Ivan Literák
Folia Parasitologica 66:003 (2019) | DOI: 10.14411/fp.2019.003
Mites of the family Pterygosomatidae are usually infesting lizards, geckos and iguanas. In this family, the genus Geckobiella Hirst, 1917 comprises 12 species including Geckobiella donnae Paredes-León, Klompen et Pérez, 2012. We collected 48 mites of G.donnae from 10 captive iguanas Ctenosaura bakeri Stejneger (Reptilia: Iguanidae) out of 23 examined individuals (prevalence 43% ) in Iguana Research and Breeding Station on Isla de Utila, Islas de la Bahía, Honduras on 1 September 2014. Males of G. donnae occurred together with females. The male of G. donnae is described here for the first time. We speculated...
Parasites of cartilaginous fishes (Chondrichthyes) in South Africa - a neglected field of marine science
Bjoern C. Schaeffner, Nico J. Smit
Folia Parasitologica 66:002 (2019) | DOI: 10.14411/fp.2019.002
Southern Africa is considered one of the world's 'hotspots' for the diversity of cartilaginous fishes (Chondrichthyes), with currently 204 reported species. Although numerous literature records and treatises on chondrichthyan fishes are available, a paucity of information exists on the biodiversity of their parasites. Chondrichthyan fishes are parasitised by several groups of protozoan and metazoan organisms that live either permanently or temporarily on and within their hosts. Reports of parasites infecting elasmobranchs and holocephalans in South Africa are sparse and information on most parasitic groups is fragmentary or entirely lacking. Parasitic...
Nanoparticles show potential to retard bradyzoites in vitro formation of Toxoplasma gondii
Oluyomi Stephen Adeyemi, Yuho Murata, Tatsuki Sugi, Yongmei Han, Kentaro Kato
Folia Parasitologica 66:001 (2019) | DOI: 10.14411/fp.2019.001
Toxoplasmosis is a common parasitic disease caused by Toxoplasma gondii (Nicolle et Manceaux, 1908), an obligate parasite capable of infecting a range of cell types in almost all warm-blooded animals. Upon infecting an intermediate host, the parasites differentiate into tachyzoites which rapidly infect host tissues. Usually, the invading parasites are cleared by the immune system and administered drugs, but some tachyzoites differentiate into bradyzoites forming tissue cysts. These tissue cysts could serve as a source for re-infection and exacerbations. Currently, treatment for toxoplasmosis is limited and, moreover, there are no drugs...