Folia Parasitologica 47[4] 267-272 (2000) | DOI: 10.14411/fp.2000.046

A 33-35 kDa circulating antigen from Plasmodium falciparum

Neirade Dominguez1, Alexis Rodriguez-Acosta2
1 Laboratory of Biochemistry, Faculty of Pharmacy, Universidad Central de Venezuela, Ciudad Universitaria, Caracas, Venezuela
2 Section of Immunochemistry, Institute of Tropical Medicine, Universidad Central de Venezuela, Ciudad Universitaria, Caracas, Venezuela

Isolation and characterisation of Plasmodium falciparum (Welch, 1897) soluble antigens from infected patient plasma, Western blotting, thermal stability and ELISA assays using hyperimmune IgG-antimalaria antibodies was the main objective of this work. A circulating antigen of approximately Mr 33-35 kDa with good specificity and antigenicity, in the plasma of malarial patients was shown. Heating at 100ºC did not destroy its antigenicity. When fractions highly enriched in the 33-35 kDa proteins were used in ELISAs, a seroreactivity in plasma obtained from primary-infected individuals was found. Controls from normal patients were always negative. The antigenic characteristics suggest that it may be included within the group of new described Plasmodium soluble antigens.

Keywords: Plasmodium falciparum, soluble antigens, malaria, excretion antigens, hyperimmune serum, thermal stability

Received: January 24, 2000; Accepted: May 25, 2000; Published: December 1, 2000  Show citation

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Dominguez, N., & Rodriguez-Acosta, A. (2000). A 33-35 kDa circulating antigen from Plasmodium falciparum. Folia Parasitologica, 47(4), 267-272. doi: 10.14411/fp.2000.046
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