Folia Parasitologica 71:006 (2024) | DOI: 10.14411/fp.2024.006

Heterocheilus floridensis sp. n. (Nematoda: Heterocheilidae) from the West Indian manatee Trichechus manatus (Trichechidae, Sirenia) in Florida, USA

František Moravec ORCID...1, Micah D. Bakenhaster ORCID...2, Seifu Seyoum ORCID...2, Michael D. Tringali ORCID...2
1 Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic;
2 Fish and Wildlife Research Institute, Florida Fish and Wildlife Conservation Commission, St. Petersburg, Florida, USA

Morphological data are used to describe a new nematode species, Heterocheilus floridensis sp. n. (Heterocheilidae) from the digestive tract of the Florida manatee Trichechus manatus latirostris (Harlan) (Trichechidae, Sirenia) from Florida, USA. Examination by light and scanning electron microscopy revealed that the new species differs from the related Heterocheilus tunicatus Diesing, 1839 mainly by having dentigerous ridges on the inner surface of the lips, a median unpaired papilla located anterior to the cloaca, and a considerably larger body size. Sequence data for subunits I and II of mitochondrial cytochrome oxidase gene, 18S small subunit and 28S ribosomal RNA genes were provided for molecular characterisation of the new species. However, the current unavailability of homologous sequence data for congeneric specimens precluded a molecular assessment of the morphological species hypothesis, and ascaridoid phylogenetic hypotheses could not be advanced. Specimens of Heterocheilus sp. collected from the Antillean manatee Trichechus manatus manatus Linnaeus in Puerto Rico, on loan from the US National Museum of Natural History, were morphologically consistent with the new species, so apparently all congeneric nematodes reported from both subspecies of the West Indian manatee Trichechus manatus Linnaeus and previously identified as H. tunicatus belong rather to H. floridensis sp. n. Heterocheilus hagenbecki (Khalil et Vogelsang, 1932) Sprent 1980 is here considered to be a species inquirenda. A key to valid species of Heterocheilus Diesing, 1839 is provided.

Keywords: Parasitic nematode, Ascaridoidea, taxonomy, aquatic mammal, marine mammal, North America

Received: August 23, 2023; Revised: January 18, 2024; Accepted: January 30, 2024; Published online: March 25, 2024  Show citation

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Moravec, F., Bakenhaster, M.D., Seyoum, S., & Tringali, M.D. (2024). Heterocheilus floridensis sp. n. (Nematoda: Heterocheilidae) from the West Indian manatee Trichechus manatus (Trichechidae, Sirenia) in Florida, USA. Folia Parasitologica, 71, Article 2024.006. https://doi.org/10.14411/fp.2024.006
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    References

    1. Anderson R.C., Chabaud A.G., Willmott S. (Eds.) 2009: Keys to the Nematode Parasites of Vertebrates. Archival Volume. CAB International, Wallingford, 663 pp. Go to original source...
    2. Bando M., Larkin I.V., Wright S.D., Greiner E.C. 2014: Diagnostic stages of the parasites of the Florida manatee, Trichechus manatus. J. Parasitol. 100: 133-138. Go to original source... Go to PubMed...
    3. Beck C.A., Forrester D.J. 1988: Helminths of the Florida manatee, Trichechus manatus latirostris, with a discussion and summary of the parasites of sirenians. J. Parasitol. 74: 628-637. Go to original source...
    4. Bonde R.K., O'Shea T.J., Beck C.A. 1983: Manual of Procedures for the Salvage and Necropsy of Carcasses of the West Indian Manatee (Trichechus manatus). National Technical Information Service, Springfield, Virginia, 175 pp.
    5. Carlson C.J., Hopkins S., Bell K.C., Doña J., Godfrey S.S., Kwak M.L., Lafferty K.D., Moir M.L., Speer K.A., Strona G., Torchin M., Wood C.L. 2020: A global parasite conservation plan. Biol. Conserv. 250: 108596. Go to original source...
    6. Černotíková E., Horák A., Moravec F. 2011: Phylogenetic relationships of some spirurine nematodes (Nematoda: Chromadorea: Rhabditida: Spirurina) parasitic in fishes inferred from SSU rRNA gene sequences. Folia Parasitol. 58: 135-148. Go to original source...
    7. De Buron I., France S.G., Connors V.A., Roumillat W.A., Tsoi L.C. 2011: Philometrids of the southern flounder Paralichthys lethostigma: a multidimensional approach to determine their diversity. J. Parasitol. 97: 466-475. Go to original source... Go to PubMed...
    8. Delgado-Estrella A., Vázquez-Maldonado V.E., Aguillar-Aguillar R. 2022: New records of nematodes from three aquatic mammals in Mexico with notes about the nematode fauna recorded in Mexican waters. Latin Amer. J. Aquat. Res. 50: 692-702. Go to original source...
    9. Diesing C.M. 1839: Neue Gattungen von Binnenwürmern nebst einem Nachtrage zur Monographie der Amphistomen. Ann. Wiener Mus. Naturgesch. 2: 219-242.
    10. Drasche R. 1884: Revision der in der Nematoden-Sammlung des k. k. zoologischen Hofcabinetes befindlichen Original-Exemplare Diesing's und Molin's. Verhandl. Kaiser.-König. Zool.-Bot. Gessellsch. Wien 33: 107-118.
    11. Gómez A., Nichols E. 2013: Neglected wild life: parasitic biodiversity as a conservation target. Int. J. Parasitol. Parasites Wildl. 2: 222-227. Go to original source... Go to PubMed...
    12. Johnson P.T.J., Preson D.L., Hoverman J.T., LaFonte B.E. 2013: Host and parasite diversity jointly control disease risk in complex communities. Proc. Natl. Acad. Sci. U.S.A. 110: 16916-16921. Go to original source... Go to PubMed...
    13. Jueco N.L. 1977: The nematode Paradujardinia halicoris in the sea cow in the Philippines. Kalikasan, Philipp. J. Biol. 6: 257-262.
    14. Khalil L.F 1984: Brevimulticaecum scleropagi sp. nov. (Ascarididae: Nematoda) from the fish Scleropages jardini in Papua New Guinea. J. Nat. Hist. 18: 797-802. Go to original source...
    15. Khalil M., Vogelsang E. G. 1932: One [sic] some nematode parasites from South American animals. Zentralbl. Bakter., Parasitenk. Infektionskrank. I. Abt. Orig. 123: 477-485.
    16. Li L., Lü L., Nadler S.A., Gibson D.I., Zhang L-P., Chen H-X., Zhao W-T., Guo Y-N. 2018: Molecular phylogeny and dating reveal a terrestrial origin in early Carboniferous for ascaridoid nematodes. Syst. Biol. 67: 888-900. Go to original source... Go to PubMed...
    17. Lucot M., Tellez M., Viveros D. 2020: Case report on helminth parasites of a necropsied Antillean manatee (Trichechus manatus manatus) in Belize. Vet. Parasitol. Regional Studies and Reports 21: 100446. Go to original source... Go to PubMed...
    18. Marcogliese D.M. 2005: Parasites of the superorganism: are they indicators of ecosystem health? Int. J. Parasitol. 35: 705-716. Go to original source... Go to PubMed...
    19. Marsh H., O'Shea T.J., Reynolds J.E. III. 2011: Ecology and Conservation of the Sirenia Dugongs and Manatees. Cambridge University Press, New York, 521 pp. Go to original source...
    20. Mašová Š., Moravec F., Baruš V., Seifertová M. 2010: Redescription, systematic status and molecular characterisation of Multicaecum heterotis Petter, Vassiliadès et Marchand, 1979 (Nematoda: Heterocheilidae), an intestinal parasite of Heterotis niloticus (Osteichthyes: Arapaimidae) in Africa. Folia Parasitol. 57: 280-288. Go to original source...
    21. Mattiucci S., Cipriani P., Webb S.C., Paoletti M., Marcer F., Bellisario B., Gibson D.I., Nascetti G. 2014: Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae). J. Parasitol. 100: 199-214. Go to original source... Go to PubMed...
    22. Mignucci-Giannoni A.A., Beck C.A., Montoya-Ospina R.A., Williams E.H. Jr. 1999a: Parasites and commensals of the West Indian manatee from Puerto Rico. J. Helminthol. Soc. Wash. 66: 67-69.
    23. Mignucci-Giannoni A.A., Williams E.H. Jr., Toyos-González G.M., Pérez-Padilla J., Rodríguez-López M.A., Vega-Guerra M.B., Ventura-González M. 1999b: Helminths from a stranded manatee in the Dominican Republic. Vet. Parasitol. 81: 69-71. Go to original source... Go to PubMed...
    24. Moravec F., Bakenhaster M.D., Seyoum S., Tringali M.D. 2021: Morphological and genetic description of two new species of philometrid nematodes (Philometridae) parasitic in needlefishes (Belonidae) from estuaries of Florida, USA. Folia Parasitol. 68: 008. Go to original source... Go to PubMed...
    25. Mozgovoy A.A. 1953: [Ascaridata of Animals and Man and the Diseases Caused by Them.] Publ. House of the USSR Acad. Sci., Moscow, 616 pp. (In Russian.)
    26. Nadler S.A. 1992: Phylogeny of some ascaridoid nematodes, inferred from comparison of 18S and 28S rRNA sequences. Mol. Biol. Evol. 9: 932-944.
    27. Nadler S.A., D'Amelio S., Fagerholm H.P., Berland B., Paggi L. 2000: Phylogenetic relationships among species of Contracaecum Railliet & Henry, 1912 and Phocascaris Höst, 1932 (Nematoda:Ascaridoidea) based on nuclear rDNA sequence data. Parasitology 121: 455-463. Go to original source... Go to PubMed...
    28. Nadler S.A., Hudspeth D.S.S. 1998: Ribosomal DNA and phylogeny of the Ascaridoidea (Nemata: Secernentea): implications for morphological evolution and classification. Mol. Phyl. Evol. 10: 221-236. Go to original source... Go to PubMed...
    29. Nadler S.A., Hudspeth D.S.S. 2000: Phylogeny of the Ascaridoidea (Nematoda: Ascaridida) based on three genes and morphology: hypothesies of structural and sequence evolution. J. Parasitol. 86: 380-393. Go to original source...
    30. Poulin R., Presswell B., Bennett J., de Angeli Dutra D., Salloum P.M. 2023: Biases in parasite biodiversity research: why some helminth species attract more research than others. Int. J. Parasitol. Parasites Wildl. 21: 89-98. Go to original source... Go to PubMed...
    31. Runge M.C., Sanders-Reed C.A., Langtimm A., Hostetler J.A., Martin J., Deutsch C.J., Ward-Geiger L.I., Mahon G.L. 2017: Status and Threats Analysis for the Florida Manatee (Trichechus manatus latirostris), 2016. U.S. Geological Survey, Reston, 40 pp. Go to original source...
    32. Shaw R.C., Greggor A.L., Plotnik J.M. 2021: The challenges of replicating research on endangered species. Anim. Behav. Cogn. 8: 240-246. Go to original source...
    33. Sprent J.F.A. 1980: Ascaridoid nematodes of sirenians - the Heterocheilinae redefined. J. Helminthol. 54: 309-327. Go to original source...
    34. Sprent J.F.A. 1983: Ascaridoid nematodes of sirenians - a new species in the Senegal manatee. J. Helminthol. 57: 69-76. Go to original source...
    35. Steller G.W. 1753: De bestiis marinis or, the Beasts of the Sea (Miller W., Miller J.E., Trans.). Zea E-Books in American Studies, 28. https://digitalcommons.unl.edu/zeaamericanstudies/28.
    36. Stiles C.W., Hassall A. 1899: Internal parasites of the fur seal. In: D.S. Jordan (Ed.), The Fur Seals and Fur-Seal Islands of the North Pacific Ocean. U.S. Government Printing Office, Washington, D.C., pp. 99-107.
    37. Tkach V.V., Kuzmin Y.I., Snyder S.D. 2010: Krefftascaris (Nematoda, Ascaridoidea) from Australian side-necked turtles with description of Krefftascaris sharpiloi sp. n. from Chelodina rugosa. Vestn. Zool. 44: 3-13. Go to original source...
    38. Trimble M.J., Van Aarde R.J. 2010: Species inequality in scientific study. Conserv. Biol. 24: 886-890. Go to original source... Go to PubMed...
    39. Weisbrod T.C., de Wit M., Hernandez J.A., Panike A.L., Rotstein D., Stacy N.I. 2021: Manatee Trichechus manatus latirostris calf mortality in Florida: a retrospective review of pathology data from 2009-2017. Dis. Aquat. Org. 147: 111-126. Go to original source... Go to PubMed...
    40. Whiteman N.K., Parker P.G. 2005: Using parasites to infer host population history: a new rationale for parasite conservation. Anim. Conserv. 8: 175-181. Go to original source...
    41. Wood C.L., Johnson T.J. 2015: A world without parasites: exploring the hidden ecology of infection. Front. Ecol. Environ. 13: 425-434. Go to original source... Go to PubMed...
    42. Wyrosdick H., Chapman A., Mignucci-Giannoni A.A., Rivera-Pérez C.I., Bonde R.K. 2018: Internal parasites of the two subspecies of the West Indian manatee Trichechus manatus. Dis. Aquat. Org. 130: 145-152. Go to original source... Go to PubMed...
    43. Zhang Y., Wang L-D., Hasegawa K., Nagae S., Chen H-X., Li L-W., Li L. 2022: Molecular identification of a new species of Rhigonema (Nematoda: Rhingonematidae) and phylogenetic relationships within the infraorder Rhigonematomorpha. Parasit. Vectors 15: 427. Go to original source... Go to PubMed...

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