Folia Parasitologica 71:024 (2024) | DOI: 10.14411/fp.2024.024

Morphological and molecular analysis of Spinitectus notopteri (Nematoda: Cystidicolidae) from freshwater fish in Thailand

Kanda Kamchoo ORCID...1, Watcharasuda Hualkasin ORCID...2, Pojchanad Pathaburee ORCID...3, Adithepchaikarn Pachanawan ORCID...4
1 Faculty of Innovative Agriculture, Fisheries and Food, Prince of Songkla University, Surat Thani Campus, Surat Thani, Thailand;
2 Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani, Thailand;
3 Office of Scientific Instrument and Testing, Prince of Songkla University, Hat Yai Campus, Songkhla, Thailand;
4 Faculty of Agriculture and Technology, Nakhon Phanom University, Nakhon Phanom, Thailand *Address for correspondence: K. Kamchoo, Faculty of Innovative Agriculture, Fisheries and Food, Prince of Songkla University, Surat Thani Campus, Surat Thani, Thailand. Email: kanda.k@psu.ac.th ORCID-iD: 0000-0002-8774-3215

The nematode Spinitectus notopteri Karve et Naik, 1951 was collected from two species of freshwater fish belonging to the family Notopteridae, namely Chitala ornata (Gray) and Notopterus notopterus (Pallas), at Khun Thale Swamp in Surat Thani province of southern Thailand. The overall prevalence of the parasite was found to be 88% (94 fish infected/106 fish examined). A higher prevalence was found in C. ornata (96%), while a higher intensity was noted for N. notopterus (117 parasites/fish). The most important morphological characters were the presence of cephalic and cuticular structures, precloacal ridges, and genital papillae. Specimens were genetically characterised using cox1 mtDNA. Morphological characteristics were most similar to Spinitectus petterae Boomker, 1993 from Clarias gariepinus (Burchell) in Africa, while the genetic data were dissimilar to all available data for the genus. Therefore, the identification of nematode specimens using a combination of morphological and molecular techniques is stressed. This study also presents the first molecular analysis of S. notopteri infecting Thai freshwater fish and a new geographical record for S. notopteri.

Keywords: Nematode, Chitala ornata, Notopterus notopterus, morphology, molecular techniques, cytochrome oxidase subunit 1 (cox1)

Received: December 21, 2023; Revised: September 30, 2024; Accepted: September 30, 2024; Published online: December 5, 2024  Show citation

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Kamchoo, K., Hualkasin, W., Pathaburee, P., & Pachanawan, A. (2024). Morphological and molecular analysis of Spinitectus notopteri (Nematoda: Cystidicolidae) from freshwater fish in Thailand. Folia Parasitologica, 71, Article 2024.024. https://doi.org/10.14411/fp.2024.024
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