Folia Parasitologica 52[3] 231-240 (2005) | DOI: 10.14411/fp.2005.031

Description of Pseudorhabdosynochus seabassi sp. n. (Monogenea: Diplectanidae) from Lates calcarifer and revision of the phylogenetic position of Diplectanum grouperi (Monogenea: Diplectanidae) based on rDNA sequence data

Xiang Y. Wu1, An X. Li1, Xing Q. Zhu2, Ming Q. Xie1
1 Centre for Parasitic Organisms, School of Life Sciences, Sun Yat-sen University, 135 Xingang West Street, Haizhu District, Guangzhou 510275, Guangdong Province, The People's Republic of China
2 Laboratory of Parasitology, College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou 510642, Guangdong Province, The People's Republic of China

Pseudorhabdosynochus seabassi sp. n. (Monogenea: Diplectanidae) from the gill filaments of Lates calcarifer Bloch, a marine teleost fish held in floating sea cages in Guangdong Province, China, is described based on morphological observations and molecular data. The shapes of the male copulatory organs (MCO) of Pseudorhabdosynochus spp. were the focus of this study. The typical proximal part of the MCO in most species of Pseudorhabdosynochus is reniform, heavily sclerotized, and divided into four chambers. However, the new species from L. calcarifer has a bulbous proximal region with four concentric layers of apparent muscular origin, instead of a reniform structure with four compartments. This organ is also different in Diplectanum grouperi Bu, Leong, Wong, Woo et Foo, 1999, being sclerotized, cup-shaped, wide proximally with four concentric muscular layers and tubular distally. The 3' terminal portion of the small subunit ribosomal RNA gene (ssrDNA) and the 5' terminal region (domains C1-D2) of the large subunit ribosomal RNA gene (lsrDNA) were used to reconstruct the phylogenetic relationships of P. seabassi and D. grouperi with related taxa utilizing maximum-parsimony and neighbour-joining methods. Phylogenetic analyses unequivocally placed D. grouperi amongst Pseudorhabdosynochus using either ssrDNA or lsrDNA data. All species of Pseudorhabdosynochus (including D. grouperi) used in this study clustered together, inferring monophyly. Based on molecular phylogenetic evidence, we propose that D. grouperi from Epinephelus coioides Hamilton be transferred to Pseudorhabdosynochus as P. grouperi comb. n.

Keywords: Pseudorhabdosynochus seabassi, Diplectanum grouperi, PCR, phylogeny, Lates calcarifer

Received: May 6, 2004; Accepted: October 1, 2004; Published: June 1, 2005  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Wu, X.Y., Li, A.X., Zhu, X.Q., & Xie, M.Q. (2005). Description of Pseudorhabdosynochus seabassi sp. n. (Monogenea: Diplectanidae) from Lates calcarifer and revision of the phylogenetic position of Diplectanum grouperi (Monogenea: Diplectanidae) based on rDNA sequence data. Folia Parasitologica, 52(3), 231-240. doi: 10.14411/fp.2005.031
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. BALASURIYA L.K.S.W. 1994: Some ecological and pathological studies of gill monogeneans in floating cage cultured seabass Lates calcarifer (Bloch). PhD thesis. University Sains Malaysia, Penang, 169 pp
    2. BALASURIYA L.K.S.W., LEONG T.S. 1995: Pseudorhabdosynochus monosquamodiscusi n. sp. (Monogenea: Diplectanidae) from Lates calcarifer cultured in floating cages in Malaysia. J. BioSci. 6: 30-34
    3. BEVERLEY-BURTON M., SURIANO D.M. 1981: A revision of Cycloplectanum Oliver, 1968 (Monogenea: Diplectanidae) and description of C. hongkongensis n. sp. and C. lantauensis n. sp. from Epinephelus spp. (Serranidae) in the South China Sea. Can. J. Zool. 59: 1276-1285 Go to original source...
    4. BU S.S.H., LEONG T.S., WONG S.Y., WOO Y.S.N., FOO R.W.T. 1999: Three diplectanid monogeneans from marine finfish (Epinephelus spp.) in the Far East. J. Helminthol. 73: 301-312 Go to original source...
    5. CHENG Q.-T., ZHEN B.-S. 1987: Systematic Synopsis of Chinese Fishes. Science Press, Beijing, 1458 pp. (In Chinese.)
    6. CHISHOLM L.A., MORGAN J.A.T., ADLARD R.D., WHITTINGTON I.D. 2001: Phylogenetic analysis of the Monocotylidae (Monogenea) inferred from 28S rDNA sequences. Int. J. Parasitol. 31: 1253-1263 Go to original source... Go to PubMed...
    7. CUNNINGHAM C.O., McGILLIVRAY D., MacKENZIE K. 1995: Phylogenetic analysis of Gyrodactylus salaris based on the small subunit (18S) ribosomal RNA gene. Mol. Biochem. Parasitol. 71: 139-142 Go to original source... Go to PubMed...
    8. DESDEVISES Y. 2001: The phylogenetic position of Furnestinia echeneis (Monogenea, Diplectanidae) based on molecular data: a case of morphological adaptation? Int. J. Parasitol. 31: 205-208 Go to original source... Go to PubMed...
    9. DESDEVISES Y., JOVELIN R., JOUSSON O., MORAND S. 2000: Comparison of ribosomal DNA sequences of La mellodiscus spp. (Monogenea, Diplectanidae) parasitising Pagellus (Sparidae, Teleostei) in the North Mediterranean Sea: species divergence and coevolutionary interactions. Int. J. Parasitol. 30: 741-746 Go to original source... Go to PubMed...
    10. DESDEVISES Y., MORAND S., JOUSSON O., LEGENDRE P. 2002: Coevolution between Lamellodiscus (Monogenea: Diplectanidae) and Sparidae (Teleostei): the study of a complex host-parasite system. Evolution 56: 2459-2471 Go to original source... Go to PubMed...
    11. DOMINGUES M.V., BOEGER W.A. 2003: Neotropical Monogenoidea. 43. Diplectanum monticellii n. sp. (Diplectanidae) from the gills of Cynoscion leiarchus (Perciformes: Sciaenidae) in Brazil. J. Parasitol. 89: 698-700 Go to original source... Go to PubMed...
    12. HUYSE T., AUDENAERT V., VOLCKAERT F.A.M. 2003: Speciation and host-parasite relationships in the parasite genus Gyrodactylus (Monogenea, Platyhelminthes) infecting gobies of the genus Pomatoschistus (Gobiidae, Teleostei). Int. J. Parasitol. 33: 1679-1689 Go to original source... Go to PubMed...
    13. JOVELIN R., JUSTINE J.-L. 2001: Phylogenetic relationships within the polyopisthocotylean monogeneans (Platyhelminthes) inferred from partial 28S rDNA sequences. Int. J. Parasitol. 31: 393-401 Go to original source... Go to PubMed...
    14. KRITSKY D.C., BEVERLEY-BURTON M. 1986: The status of Pseudorhabdosynochus Yamaguti, 1958 and Cycloplectanum Oliver, 1968 (Monogenea: Diplectanidae). Proc. Biol. Soc. Wash. 99: 17-20
    15. LEONG T.S. 1994: Parasites and diseases of cultured marine fin fishes in South East Asia. University Sains Malaysia, Penang, 25 pp
    16. LEONG T.S., WONG S.Y. 1990: Parasites of healthy and diseased juvenile grouper (Epinephelus malabaricus Bloch & Schneider) and seabass (Lates calcarifer Bloch) in floating cages in Penang, Malaysia. Asian Fish. Sci. 3: 319-327 Go to original source...
    17. LIANG K.S., LEONG T.S. 1991: A redescription of Pseudorhabdosynochus latesi (Tripathi 1955) and description of Diplectanum penangi n. sp. from Lates calcarifer cultured in floating cages in Malaysia and Thailand. J. BioSci. 2: 77-84
    18. LITTLEWOOD D.T.J., ROHDE K., BRAY R.A. 1999: Phylogeny of the Platyhelminthes and the evolution of parasitism. Biol. J. Linn. Soc. 68: 257-287 Go to original source...
    19. LITTLEWOOD D.T.J., ROHDE K., CLOUGH K.A. 1998: The phylogenetic position of Udonella (Platyhelminthes). Int. J. Parasitol. 28: 1241-1250 Go to original source... Go to PubMed...
    20. LITVAITIS M.K., ROHDE K., 1999: A molecular test of platyhelminth phylogeny: inferences from partial 28S rDNA sequences. Invertebr. Biol. 118: 42-45 Go to original source...
    21. MO T.A. 1991: Variations of opisthaptoral hard parts of Gyrodactylus salaris Malmberg, 1957 (Monogenea, Gyrodactylidae) on parr of Atlantic salmon (Salmo salar L.) in laboratory experiments. Syst. Parasitol. 20: 11-19 Go to original source...
    22. MOLLARET I., JAMIESON B.G.M., ADLARD R.D., HUGALL A., LECOINTRE G., CHOMBARD C., JUSTINE J.-L. 1997: Phylogenetic analysis of the Monogenea and their relationships with Digenea and Eucestoda inferred from 28S rDNA sequences. Mol. Biochem. Parasitol. 90: 433-438 Go to original source... Go to PubMed...
    23. MOLLARET I., JAMIESON B.G.M., JUSTINE J.-L. 2000: Phylogeny of the Monopisthocotylea and Polyopisthocotylea (Platyhelminthes) inferred from 28S rDNA sequences. Int. J. Parasitol. 30: 171-185 Go to original source... Go to PubMed...
    24. NADLER S.A., ADAMS B.J., LYONS E.T., DELONG R.L., MELIN S.R. 2000: Molecular and morphometric evidence for separate species of Uncinaria (Nematoda: Ancylostomatidae) in California sea lions and northern fur seals: hypothesis testing supplant verification. J. Parasitol. 86: 1099-1106 Go to original source... Go to PubMed...
    25. NASH G., ANDERSON I.G., SHARIFF M., SHAMSUDIN M.N. 1987: Bacteriosis associated with epizootic in giant sea perch, Lates calcarifer, and the estuarine grouper, Epinephelus tauvina, cage cultured in Malaysia. Aquaculture 67: 105-111 Go to original source...
    26. OLIVER G. 1968: Recherches sur les Diplectanidae (Monogenea) parasites de Teleosteens du golfe du Lion. I. Diplectaninae Monticelli, 1903. Vie Milieu, ser. A, Biol. Mar., 19: 95-138
    27. OLIVER G. 1987: Les Diplectanidae Bychowsky, 1957 (Monogenea, Monopisthocotylea, Dactylogyridea). Systematique. Biologie. Ontogenie. Ecologie. Essai de phylogenese. Universite des Sciences et Techniques du Languedoc, Montpellier, These de Doctorat d'Etat, mention Sciences, 443 pp
    28. OLIVER G. 1993: Les Diplectanidae Bychowsky, 1957 (Monogenea, Monopisthocotylea, Dactylogyridea) marqueurs biologiques, temoins de la biogeographie et de l'evolution de leurs hotes. Bull. Soc. Zool. Fr. 118: 25-36
    29. OLSON P.D., LITTLEWOOD D.T.J. 2002: Phylogenetics of the Monogenea - evidence from a medley of molecules. Int. J. Parasitol. 32: 233-244 Go to original source... Go to PubMed...
    30. SANTOS C.P., BUCHMANN K., GIBSON D.I. 2000: Pseudorhabdosynochus spp. (Monogenea: Diplectanidae) from the gills of Epinephelus spp. in Brazilian waters. Syst. Parasitol. 45: 145-153 Go to original source... Go to PubMed...
    31. SIMKOVA A., PLAISANCE L., MATEJUSOVA I., MORAND S., VERNEAU O. 2003: Phylogenetic relationships of the Dactylogyridae Bychowsky, 1933 (Monogenea: Dactylogyridea): the need for the systematic revision of the Ancyrocephalinae Bychowsky, 1937. Syst. Parasitol. 54: 1-13 Go to original source... Go to PubMed...
    32. THOMPSON J.D., GIBSON T.J., PLEWNIAK F., JEANMOUGIN F., HIGGINS D.G. 1997: The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids Res. 24: 4876-4882 Go to original source... Go to PubMed...
    33. WU B.-H., LONG S., WANG W.-J. 2000: Fauna Sinica: Platyhelminthes Monogenea. Science Press, Beijing, China, 756 pp. (In Chinese.)
    34. YAMAGUTI S. 1963: Systema Helminthum. Vol. 4. Monogenea and Aspidocotylea. Interscience Publishers, New York, London, 699 pp
    35. ZHANG J.-Y., YANG T.-B., LIU L. 2001: [Monogeneans of Chinese Marine Fishes.] Agriculture Press, Beijing, China, 400 pp. (In Chinese.)

    Return to the content