Folia Parasitologica 73:001 (2026) | DOI: 10.14411/fp.2026.001

Three decades of research on molecular genetic characteristics of Echinococcus spp.: a bibliometric study

Aidana Tautanova ORCID...1*, Vladimir Kiyan ORCID...2, Marat Dusmagambetov ORCID...1, Tanzilya Almysheva ORCID...1, Aigul Dusmagambetova ORCID...1
1 Department of Microbiology and Virology, Astana Medical University, Astana, Kazakhstan;
2 Laboratory of Biodiversity and Genetic Resources National Center for Biotechnology, Astana, Kazakhstan

Research on the molecular genetics of Echinococcus has advanced significantly owing to its relevance in understanding genetic variation, host-parasite interactions, and the transmission of Echinococcus species. A bibliometric analysis was performed using RStudio (Bibliometrix) and VOSviewer to evaluate molecular genetic research on Echinococcus spp. published between 1985 and 2025. Data were extracted from Scopus, Web of Science, PubMed, Europe PMC, and ProQuest. The analysis revealed a steady rise in publications on Echinococcus molecular genetics, accelerating from the early 2000s and peaking around 2017, with a slight decline after 2023, reflecting fluctuations in research activity. Comparative analysis showed Echinococcus consistently surpassed Schistosoma, Taenia, and soil-transmitted helminths, peaking at 60 articles in 2020-2021. Early publications achieved high mean Total Citations (TC) per year, followed by a stable trend, indicating lasting impact. Leading journals included Parasitology Research (Germany; 70 articles, H-index 114), Veterinary Parasitology (Netherlands; 62, H-index 150), Parasitology (UK; 53, H-index 173), Acta Tropica (Netherlands; 34, H-index 122), and PLOS Neglected Tropical Diseases (USA; 28, H-index 172). Tehran University of Medical Sciences (54 articles) led institutions. Influential authors included D. P. McManus and T. Romig. Multinational studies (484 publications) had higher citations than single-country (474). Iran (121), China (116), and Italy (53) led global contributions, emphasising collaboration, genotyping, phylogeny, and veterinary applications. This bibliometric study underscores the evolving trends in Echinococcus molecular genetics, providing valuable insights for researchers and stakeholders to guide future investigations and collaborative strategies in controlling echinococcosis.

Keywords: Cestoda, Genotypes, host interactions, genetic variation, drug resistance, epidemiology, zoonoses.

Received: September 5, 2025; Revised: January 2, 2026; Accepted: January 29, 2026; Published online: February 24, 2026  Show citation

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Tautanova, A., Kiyan, V., Dusmagambetov, M., Almysheva, T., & Dusmagambetova, A. (2026). Three decades of research on molecular genetic characteristics of Echinococcus spp.: a bibliometric study. Folia Parasitologica, 73, Article 2026.001. https://doi.org/10.14411/fp.2026.001
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    References

    1. Ab Rashid M.F. 2023: How to conduct a bibliometric analysis using R packages: a comprehensive guideline. J. Tour. Hospit. Cul. Arts. 15: 24-39.
    2. Abdybekova A.M., Zhang Z., Sultanov A.A., Abdibayeva A.A., Zhaksylykova A.A., Junisbayeva S.M., Aubakirov M.Z., Akhmetova G.D., Torgerson P.R. 2019: Genotypes of Echinococcus isolated from domestic livestock in Kazakhstan. J. Helminthol. 94: e69.
    3. Ablakimova N., Smagulova G.A., Rachina S., Mussina A.Z., Zare A., Mussin N.M., Zare A., Mussin N.M., Kaliyev A.A., Shirazi R., Tanideh N., Tamadon A. 2023: Bibliometric analysis of global research output on antimicrobial resistance among pneumonia pathogens (2013-2023). Antibiotics 12: 1411.
    4. Akyuz M., Kirman R., Guven E., Balkaya I., Avcioglu H. 2024: Echinococcus granulosus sensu stricto and Echinococcus multilocularis in a gray wolf (Canis lupus) in Turkey: further evidence for increased risk of alveolar echinococcosis in urban areas. Acta Parasitol. 69: 1319-1323.
    5. Ali R., Nazeer S., Elahi M.M.S., Idu E.G., Zhang H., Mahmoudvand H., Khan S.N., Yang J. 2024: Global distribution and definitive host range of Echinococcus species and genotypes: a systematic review. Vet. Parasitol. 331: 110273.
    6. Almulhim AM, John S. 2023: Echinococcus granulosus. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK539751/.
    7. Alvi M.A., Alsayeqh A.F. 2022: Food-borne zoonotic echinococcosis: a review with special focus on epidemiology. Front. Vet. Sci. 9: 1072730.
    8. Alvi M.A., Ohiolei J.A., Saqib M., Li L., Tayyab M.H., Alvi A.A., Wu Y.T., Fu B.Q., Yan H.B., Jia W.Z. 2020: Echinococcus granulosus (sensu stricto) (G1, G3) and E. ortleppi (G5) in Pakistan: phylogeny, genetic diversity and population structural analysis based on mitochondrial DNA. Parasit. Vectors 13: 316.
    9. Ansorge L. 2024: Bibliometric studies as a publication strategy. Metrics 1: 5.
    10. Bakhtiar N.M., Spotin A., Mahami-Oskouei M., Ahmadpour E., Rostami A. 2020: Recent advances on innate immune pathways related to host-parasite cross-talk in cystic and alveolar echinococcosis. Parasit. Vectors 13: 232.
    11. Barazesh A., Sarkari B., Shahabi S., Halidi A.G., Ekici A., Aydemir S., Mahami-Oskouei M. 2020: Genetic diversity of Echinococcus granulosus isolated from humans: a comparative study in two cystic echinococcosis endemic areas, Turkey and Iran. Biomed. Res. Int. 2020: 3054195.
    12. Borhani M., Fathi S., Harandi M.F., Casulli A., Ding J., Liu M.,Zhang W., Wen H. 2024: Echinococcus granulosus sensu lato control measures: a specific focus on vaccines for both definitive and intermediate hosts. Parasit. Vectors 17: 533.
    13. Casulli A., Abela-Ridder B., Petrone D., Fabiani M., Bobiæ B., Carmena D., ©oba B., Zerem E., Gargaté M.J., Kuzmanovska G., Calomfirescu C. 2023: Unveiling the incidences and trends of the neglected zoonosis cystic echinococcosis in Europe: a systematic review from the MEmE project. Lancet Infect. Dis. 23: e95-e107.
    14. CDC 2019: Echinococcosis. Centers for Disease Control and Prevention. Available from: https://www.cdc.gov/dpdx/echinococcosis/index.html
    15. De Lima C., Paiva B.S.R., Dos Santos Neto M.F., Hui D., Perez-Cruz P.E., Zimmermann C., Bruera E., Paiva C.E. 2021: The impact of international research collaborations on the citation metrics and the scientific potential of South American palliative care research: bibliometric analysis. Ann. Glob. Health 87: 29.
    16. Deol A.K., Fleming F.M., Calvo-Urbano B., Walker M., Bucumi V., Gnandou I., Tukahebwa E.M., Jemu S., Mwingira U.J., Alkohlani A., Traoré M. 2019: Schistosomiasis - assessing progress towards the 2020 and 2025 global goals. N. Engl. J. Med. 381: 2519-2528.
    17. Dietrich C.F., Douira-Khomsi W., Gharbi H., Sharma M., Cui X.W., Sparchez Z., Richter J., Kabaalioğlu A., Atkinson N.S., Schreiber-Dietrich D., Dong Y. 2020: Cystic echinococcosis, review and illustration of non-hepatic manifestations. Med. Ultrason. 22: 319-324.
    18. Donthu N., Kumar S., Mukherjee D., Pandey N., Lim W.M. 2021: How to conduct a bibliometric analysis: an overview and guidelines. J. Bus. Res. 133: 285-296.
    19. Dorjsuren T., Narantsogt G., Dagvasumberel M., Ganbat M., Sumiya G., Damdinsuren B., Tsevelvaanchig U., Boldbaatar C., Jamiyansuren J., Orgoi S., Ayushkhuu T. 2019: The status of human echinococcosis in Western Mongolia. Centr. Asian J. Med. Sci. 5: 30-39.
    20. Duarte I., Santos H.G.G., Rodrigues M.L. 2025: A general analysis of the impact of international collaboration on the citation indices of scientific publications from 60 institutions across five continents. Anais Acad. Bras. Cienc. 97: e20241035.
    21. Ellis J.T., Ellis B., Velez-Estevez A., Reichel M.P., Cobo M.J. 2020: 30 years of parasitology research analysed by text mining. Parasitology 147: 1643-1657.
    22. Fakhar M., Keighobadi M., Hezarjaribi H.Z., Montazeri M., Banimostafavi E.S., Sayyadi S., Ghaffari Hamadani M.M.G., Sharifpour A., Tabaripour R., Asadi S., Soosaraei M., Khasseh A.A. 2021: Two decades of echinococcosis/hydatidosis research: bibliometric analysis based on the Web of Science core collection databases (2000-2019). Food Waterborne Parasitol. 25: e00137.
    23. Fu M., Han S., Xue C., Wang X., Liu B., Wang Y., Wang L., Wei S., Cui X., Zhang T., Zhang H.B., Zheng B., Tian T., Yang S., Gao C.H., Dang Z., Xu B., Yu Q., Wu W. 2020: Contribution to the echinococcosis control programme in China by NIPD-CTDR. Adv. Parasitol. 110: 107-144.
    24. Gottstein B., Deplazes P. 2021: Alveolar echinococcosis: what triggers emergence in North America, Central Europe and Asia? Curr. Opin. Infect. Dis. 34: 440-446.
    25. Han X., Jian Y., Zhang X., Ma L., Zhu W., Cai Q., Wu S., Wang X., Shi B. 2019: Genetic characterization of Echinococcus isolates from various intermediate hosts in the Qinghai-Tibetan Plateau area, China. Parasitology 146: 1305-1312.
    26. Haniloo A., Jamshidi A., Ghatee M.A., Fazaeli A. 2021: Sequence analysis, intra-genotyping variation, and phylogenetic study of nad1 gene in Echinococcus granulosus sensu lato genotypes from intermediate hosts in southwestern Iran. Vet. Parasitol. Reg. Stud. Rep. 26: 100655.
    27. Herz M., Zarowiecki M., Wessels L., Pätzel K., Herrmann R., Braun C., Holroyd N., Huckvale T., Bergmann M., Spiliotis M., Koziol U., Berriman M., Brehm K. 2024: Genome-wide transcriptome analysis of Echinococcus multilocularis larvae and germinative cell cultures reveals genes involved in parasite stem cell function. Front. Cell. Infect. Microbiol. 14: 1335946.
    28. Ioannidis J.P.A., Collins T.A., Bendavid E., Baas J. 2025: Massive covidization and subsequent decovidization of the scientific literature involved 2 million authors. J. Clin. Epidemiol. 180: 111705.
    29. Kakundi E.M. 2020: Molecular epidemiology of Echinococcus and Taenia species in dogs from cystic echinococcosis endemic areas in Kenya. PhD thesis, University of Nairobi, Nairobi, 209 pp.
    30. Karima A., Manel A., Sara B., Assia H., Abdelkarim L., Moussa L., Wen H. 2020: International consensus on terminology to be used in the field of echinococcoses. Parasite 27: 41.
    31. Khan S., Cable J., Masud N., Hailer F., Younus M., Hussain N., Asif Idrees M., Rashid M.I., Akbar H. 2025: Epidemiological and genotypic assessment of cystic echinococcosis in ruminant populations of Northern Punjab, Pakistan: a neglected zoonotic disease. Parasitol. Res. 124: 7.
    32. Laatamna A.E., Ebi D., Brahimi K., Bediaf K., Wassermann M., Souttou K., Romig T. 2019: Frequency and genetic diversity of Echinococcus granulosus sensu stricto in sheep and cattle from the steppe region of Djelfa, Algeria. Parasitol. Res. 118: 89-96.
    33. Labanchi J.L., Poggio T.V., Gutiérrez A., Mujica G., Araya D., Grizmado C., Calabro A., Crowley P., Arezo M., Seleiman M., Herrero E., Sepulveda L., Talmon G., Diaz O., Larrieu E. 2022: Analysis of vaccination strategy against cystic echinococcosis developed in the Province of Río Negro, Argentina: 12 years of work. Vet. Parasitol. 310: 109795.
    34. Laurimäe T., Kinkar L., Moks E., Bagrade G., Saarma U. 2023: Exploring the genetic diversity of genotypes G8 and G10 of the Echinococcus canadensis cluster in Europe based on complete mitochondrial genomes (13 550-13 552 bp). Parasitology 150: 631-637.
    35. Mahmoudi S., Mamishi S., Banar M., Pourakbari B., Keshavarz H. 2019: Epidemiology of echinococcosis in Iran: a systematic review and meta-analysis. BMC Infect. Dis. 19: 929.
    36. Mandt R., Seetharam K., Chang C.H.M. 2020: Federal R & D funding: the bedrock of national innovation. MIT Sci. Policy. Rev. 1: 44-54.
    37. Manterola C., Totomoch-Serra A., Rojas C., Riffo-Campos Á.L., García-Méndez N. 2021: Echinococcus granulosus sensu lato genotypes in different hosts worldwide: a systematic review. Acta Parasitol. 67: 161-185.
    38. Maurice A.P., Jenkin A., Norton R.E., Hamilton A., Ho Y.H. 2020: Epidemiology of parasitic diseases. In: G. Tsoulfas, J. Hoballah, G. Velmahos, Y.H. Ho (Eds.), The Surgical Management of Parasitic Diseases. Springer, Cham, pp. 3-20.
    39. Meirmanova A., Omarova G., Kurmanova A., Begniyazova Z., Yuldasheva A. 2023: Surgical management of genital prolapse and combined gynecological pathologies in women: a meta-analysis. Electron. J. Gen. Med. 20: em505.
    40. Meirmans S., Butlin R.K., Charmantier A., Engelstädter J., Groot A.T., King K.C., Kokko H., Reid J.M., Neiman M. 2019: Science policies: how should science funding be allocated? An evolutionary biologists' perspective. J. Evol. Biol. 32: 754-768.
    41. Moradi M., Meamar A.R., Akhlaghi L., Roozbehani M., Razmjou E. 2019: Detection and genetic characterization of Echinococcus granulosus mitochondrial DNA in serum and formalin-fixed paraffin-embedded cyst tissue samples of cystic echinococcosis patients. PLoS One 14: e0224501.
    42. Muric G., Lerman K., Ferrara E. 2021: Gender disparity in the authorship of biomedical research publications during the COVID-19 pandemic: retrospective observational study. J. Med. Internet Res. 23: e25379.
    43. Nagasbekov M.S., Baimakhanov Z.B., Nurlanbayev E.K., Kaniyev S.A., Chormanov A.T., Baimakhanov B.B. 2022: Modern approaches in the diagnostics and treatment of cystic liver echinococcosis. Literature review. Kazakh. J. Surg. 73: 36-42.
    44. Omarova G., Sultanova Z., Aimbetova A., Saduakassova S., Yuldasheva A. 2024: Cesarean section: medical, social and moral and ethical factors. Salud. Cienc. Tecnol. 4: 113.
    45. Page M.J., McKenzie J.E., Bossuyt P.M., Boutron I., Hoffmann T.C., Mulrow C.D., Shamseer L., Tetzlaff J.M., Akl E.A., Brennan S.E., Chou R., Glanville J., Grimshaw J.M., Hróbjartsson A., Lalu M.M., Li T., Loder E.W., Mayo-Wilson E., McDonald S., McGuinness L.A., Stewart L.A., Thomas J., Tricco A.C., Welch V.A., Whiting P., Moher D. 2021: The PRISMA 2020 statement: an updated guideline for reporting systematic reviews. BMJ 372: n71.
    46. Pal M., Alemu H.H., Marami L.M., Garedo D.R., Bodena E.B. 2022: Cystic echinococcosis: a comprehensive review on life cycle, epidemiology, pathogenesis, clinical spectrum, diagnosis, public health and economic implications, treatment, and control. Med. Sci. Monit. 28: e934912.
    47. Paternoster G., Boo G., Wang C., Minbaeva G., Usubalieva J., Raimkulov K.M., Zhoroev A., Abdykerimov K.K., Kronenberg P.A., Müllhaupt B., Furrer R., Deplazes P., Torgerson P.R. 2020: Epidemic cystic and alveolar echinococcosis in Kyrgyzstan: an analysis of national surveillance data. Lancet Glob. Health 8: e603-e611.
    48. Pesquisa FAPESP 2024: Drop in number of scientific articles published worldwide. Pesquisa FAPESP. Available from: www.revistapesquisa.fapesp.br/en/drop-in-number-of-scientific-articles-published-worldwide.
    49. Rachel F., Luttermann C., Höper D., Conraths F.J., Dapprich J., Maksimov P. 2025: Typing of Echinococcus multilocularis by region-specific extraction and next-generation sequencing of the mitogenome. Front. Microbiol. 16: 1535628.
    50. Romig T., Wassermann M. 2024: Echinococcus species in wildlife. Int. J. Parasitol. Parasites Wildl. 23: 100913.
    51. Rostami A., Lundström-Stadelmann B., Frey C.F., Beldi G., Lachenmayer A., Chang B.C.H., Norouzian M.M., Hemphill A., Gasser R.B. 2025: Human alveolar echinococcosis - a neglected zoonotic disease requiring urgent attention. Int. J. Mol. Sci. 26: 2784.
    52. ªahin S., Kaya B. 2025: Evaluation of hydatid cyst cases: a single-center retrospective study. Turkiye Parazitol. Derg. 48: 222-227.
    53. Sánchez-Marqués R., Mas-Coma S., Salas-Coronas J., Boissier J., Bargues M.D. 2022: Research on schistosomiasis in the era of the COVID-19 pandemic: a bibliometric analysis. Int. J. Environ. Res. Public Health 19: 8051.
    54. Santa M.A., Pastran S., Klein C., Ruckstuhl K., Massolo A. 2019: Evaluation of an automated magnetic bead-based DNA extraction and real-time PCR in fecal samples as a pre-screening test for detection of Echinococcus multilocularis and Echinococcus canadensis in coyotes. Parasitol. Res. 118: 119-125.
    55. Seitkamzina D., Akmambaeva B., Abulgazimova G., Yelemessova B., Ussenbayev A., Omarov K., Tokaeva M., Zhakiyanova Y., Zhanabayev A. 2023: Prevalence of endo- and ectoparasitism of sheep in Northern Kazakhstan. Am. J. Anim. Vet. Sci. 3: 223-228.
    56. Shams M., Khazaei S., Naserifar R., Shariatzadeh S.A., Anvari D., Montazeri F., Pirestani M., Majidiani H. 2022: Global distribution of Echinococcus granulosus genotypes in domestic and wild canids: a systematic review and meta-analysis. Parasitology 149: 1147-1159.
    57. Siyadatpanah A., Anvari D., Zeydi A.E., Hosseini S.A., Daryani A., Sarvi S., Budke C.M., Dizaji R.E., Mohaghegh M.A., Kohansal M.H., Dodangeh S. 2019: A systematic review and meta-analysis of the genetic characterization of human echinococcosis in Iran, an endemic country. Epidemiol. Health 41: e2019024.
    58. Szydlowski N. 2025: Library science literature, 2019-2025: an exploration using critical bibliometric methods. J. Acad. Librarianship 51: 103142.
    59. Thompson R.C.A. 2020: The molecular epidemiology of Echinococcus infections. Pathogens 9: e00075-18.
    60. Tulading A., Wu J., Zhang Y.F., Mamuti A., Azhati Y., Lv C.H., Tuersunmaimaiti A., Tuxun T. 2025: Immunological landscape of hepatic alveolar echinococcosis: a bibliometric analysis. World J. Hepatol. 17: 102001.
    61. Uakhit R., Smagulova A., Syzdykova A., Abdrakhmanov S., Kiyan V. 2022: Genetic diversity of Echinococcus spp. in wild carnivorous animals in Kazakhstan. Vet. World 15: 1489-1496.
    62. Ullah I., Sattar S., Ali I., Farid A., Ullah A., Eid R.A., Samir A. Zaki M., Alaa Eldeen M., Ahmed I., Ullah I. 2023: Molecular epidemiology of cystic echinococcosis in rural Baluchistan, Pakistan: a cross-sectional study. Pathogens 12: 40.
    63. Wang J., Frietsch R., Neuhäusler P., Hooi R. 2024: International collaboration leading to high citations: global impact or home country effect? J. Informetrics 18: 101565.
    64. Wang X., Zhu A., Cai H., Liu B., Xie G., Jiang R., Zhang J., Xie N., Guan Y., Bergquist R., Wang Z., Li Y., Wu W. 2021: The pathology, phylogeny, and epidemiology of Echinococcus ortleppi (G5 genotype): a new case report of echinococcosis in China. Infect. Dis. Poverty 10: 130.
    65. Wen H., Vuitton L., Tuxun T., Li J., Vuitton D.A., Zhang W., McManus D.P. 2019: Echinococcosis: advances in the 21st century. Clin. Microbiol. Rev. 32: e00075-18.
    66. WHO 2021: Ending the neglect to attain the Sustainable Development Goals: a road map for neglected tropical diseases 2021-2030. World Health Organization. Available from: https://www.who.int/publications/i/item/9789240010352
    67. WHO 2023: Schistosomiasis. World Health Organization. Available from: https://www.who.int/news-room/fact-sheets/detail/schistosomiasis
    68. Woolsey I.D., Miller A.L. 2021: Echinococcus granulosus sensu lato and Echinococcus multilocularis: a review. Res. Vet. Sci. 135: 517-522.
    69. Zeghir-Bouteldja R., Touil-Boukoffa C. 2022: Identification of proteins of laminated layer of Echinococcus granulosus: interface among host and parasite. Veterinaria 71: 53-60.
    70. Zhao Y., Gesang D., Wan L., Li J., Qiangba G., Danzeng W., Basang Z., Renzhen N., Yin J., Gongsang Q., Cai H., Pang H., Wang D., Asan, Zhang Q., Chen W. 2022: Echinococcus spp. and genotypes infecting humans in Tibet Autonomous Region of China: a molecular investigation with near-complete/complete mitochondrial sequences. Parasit. Vectors 15: 75.
    71. Zhou G., Li Z., Zhang X., Meng R., Shen X., Zhang Z., Chen F., Duo H., Fu Y. 2025: Molecular epidemiology and phylogenetic analysis of Echinococcus spp. in the Qinghai-Tibetan Plateau: unraveling genetic evolution in a high-risk region for echinococcosis. Vet. Res. Commun. 49: 252.

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