Folia Parasitologica 55[1] 75-78 (2008) | DOI: 10.14411/fp.2008.010

A diagnostic assay based on variable intergenic region distinguishes between Leishmania donovani and Leishmania infantum

Eva Chocholová1,2,3, Milan Jirků1, Julius Lukeš1
1 Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic
2 Present address: Centre for Cellular and Molecular Neurobiology, University of Liege, B-4000 Liege, Belgium
3 Previous name: Eva Zemanová

We have developed a PCR assay that in a single reaction distinguishes between Leishmania infantum and Leishmania donovani strains on the basis of different size of the amplicon. The targeted intergenic region between putative biopterin transporter and nucleotide binding protein on chromosome 35 is highly variable, species-specific and can be amplified from clinical samples. Based on the assay, five tested Leishmania archibaldi and L. infantum strains from the Sudan and Ethiopia clearly belong to L. donovani, which is in accordance with a recent multifactorial analysis of these strains. The nucleotide sequence reported in this paper has been submitted to the GenBankTM with accession number EU068004.

Received: July 24, 2007; Accepted: October 3, 2007; Published: March 1, 2008  Show citation

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Chocholová, E., Jirků, M., & Lukeš, J. (2008). A diagnostic assay based on variable intergenic region distinguishes between Leishmania donovani and Leishmania infantum . Folia Parasitologica, 55(1), 75-78. doi: 10.14411/fp.2008.010
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