Folia Parasitologica, vol. 46 (1999), issue 3

Entamoeba histolytica: surface proteolytic activity and its relationship with in vitro virulence

Luz María Araiza-Orozco, Eva Edilia Avila, María de Lourdes Muñoz, Sergio Arias-Negrete

Folia Parasitologica 46[3] 161-167 (1999)

We determined the surface-associated proteolytic activity in three Entamoeba histolytica Schaudinn, 1903 strains (monoxenic HM1, axenic HM1, and HK9) of known virulence and its relationship with collagenase activity. Both activities were also determined in axenic HM1 amoebae trophozoites which were sensitive and resistant to complement-mediated lysis. Surface proteolytic activity was determined in glutaraldehyde-fixed E. histolytica trophozoites, which degraded the insoluble substrate, hide powder azure, and cleaved the human immunoglobulin G heavy chain in a time-dependent fashion, at neutral pH, in presence of 2-mercaptoethanol as...

Eimeria nycteae sp. n. (Apicomplexa: Eimeriidae), a new parasite species from the snowy owl, Nyctea scandiaca

Jiří Volf, Břetislav Koudela, David Modrý

Folia Parasitologica 46[3] 168-170 (1999)

Coprological examinations of three snowy owls, Nyctea scandiaca (L.) revealed the presence of a coccidium of the genus Eimeria that apparently represents a previously undescribed species. Oocysts of Eimeria nycteae sp. n. were spherical to subspherical, 23.6 (23-25) × 22.2 (22-23) µm with a shape index 1.1 (1.0-1.1). The oocyst wall was bilayered, smooth ~ 0.75 µm thick. A polar granule was absent. Sporocysts were ellipsoidal, 18.5 (18-19) × 9.8 (9-10) µm with a shape index 1.9 (1.8-2.1) with Stieda and substieda bodies. A sporocyst residuum was present as small granules scattered among sporozoites. The sequence...

Effect of low and high temperatures on infectivity of Encephalitozoon cuniculi spores suspended in water

Břetislav Koudela, Štěpánka Kučerová, Tomáš Hudcovic

Folia Parasitologica 46[3] 171-174 (1999)

The survival of Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923 spores suspended in distilled water and exposed at defined temperatures was investigated. Infectivity of E. cuniculi spores was tested by inoculation of SCID mice. There was no marked loss of infectivity of spores stored at 4°C for two years or frozen at -12°C and -24°C for 1, 8, and 24 h. Although there was a remarkable loss of infectivity, spores remained infective after freezing at -70°C for 1 and 8 h. Heating at 60°C and 70°C for 5 min and 1 min, respectively, rendered the microsporidia non-infective. These findings demonstrate that E. cuniculi spores...

Globidia infection in the gut of an Australian gecko Heteronotia binoei

Ilan Paperna

Folia Parasitologica 46[3] 175-178 (1999)

Globidia, at various stages of differentiation were found in histological sections prepared from the digestive tract of an Australian gecko Heteronotia binoei Gray, 1845. The globidia - seemingly in hypertrophic endothelial host cells - were located in the lamina propria of the small intestine, and were enclosed in a parasitophorous vacuole with various stages of differentiating meronts. When fully matured, globidia contained hundreds of merozoites within their parasitophorous vacuole.

Transmission of the monogenean Gyrodactylus salaris

Arnulf Soleng, Peder A. Jansen, Tor A. Bakke

Folia Parasitologica 46[3] 179-184 (1999)

The present study is focusing on the transmission of the monogenean ectoparasite Gyrodactylus salaris Malmberg, 1957, a major pathogen on natural populations of Norwegian Atlantic salmon, Salmo salar L. In laboratory experiments the transmission rate of G. salaris after direct host to host contact was positively correlated with water temperature (1.2, 4.7 and 12.2°C). The transmission of detached G. salaris in the planktonic drift was studied in field experiments where salmon parr were individually isolated for 24 hours in small wire mesh cages suspended in the water column. Ten out of 157 salmon parr (prevalence 6.4%, mean...

Morphology and taxonomy of Stomylotrema (Digenea: Stomylotrematidae) representatives from ciconiiform and podicipediform birds in Cuba

Jozef Kazimír Macko, Marta Špakulová, Juan Carlos Casanova

Folia Parasitologica 46[3] 185-190 (1999)

Two Stomylotrema Looss, 1900 species were found in storks and grebes in Cuba. Five specimens of Stomylotrema bijugum Braun, 1901 were recovered from the roseate spoonbill, Ajaia ajaja (Allen, 1942) (Ciconiiformes) while six Stomylotrema vicarium Braun, 1901 were found in the little blue heron, Egretta (syn. Florida) caerulea (L.) (Ciconiiformes) and the least grebe Podiceps dominicus dominicus (L.) (Podicipediformes). The taxa represent new host and geographical records. The problem of morphological variation within the genus Stomylotrema is briefly analysed in the discussion.

Ultrastructure of spermiogenesis of Phyllobothrium lactuca (Cestoda, Tetraphyllidea, Phyllobothriidae)

Aminata Sène, Cheikh Tidiane Bâ, Bernard Marchand

Folia Parasitologica 46[3] 191-198 (1999)

Spermiogenesis in Phyllobothrium lactuca Beneden, 1850 begins with the formation of a differentiation zone bordered by cortical microtubules and containing a nucleus and two centrioles separated by an intercentriolar body and disposed one in the prolongation of the other. Later, formation of flagellar buds, striated roots and a median cytoplasmic extension takes place. Each centriole gives rise to a flagellum that rotates and fuses with the median cytoplasmic extension. At this stage, arched membranes appear at the front of the differentiation zone. The nucleus elongates, becomes filiform and migrates between the striated roots into the spermatid....

Ultrastructure of glands in a scutariellid (Platyhelminthes) and possible phylogenetic implications

Carlo Iomini, Marco Ferraguti, Jean-Lou Justine

Folia Parasitologica 46[3] 199-203 (1999)

Subepidermal glands of the body of Troglocaridicola sp. (from the cavernicolous shrimp Troglocaris sp. in eastern Italy) were observed by transmission electron microscopy. The reservoir and duct of the glands are lined with longitudinal microtubules. Membrane-bound granules inside the gland show a distinctive pattern: they contain fibres, 18 nm in diameter, regularly arranged in bundles with a 5 nm space between fibres. From a survey of the available literature on glands of Platyhelminthes, it is concluded that this structure is known only in this species. Glands with regularly arranged 18 nm fibres, if characteristic for the Scutariellidae,...

R.V. Cohen, F. Aun (Eds.): Tropical Surgery. A vademecum series book.

Jaroslav Gutvirth

Folia Parasitologica 46[3] 204 (1999)

Karger Landes Systems, Basel, Switzerland, 1997, ISBN 3-8055-6497-X, soft cover. 195 figs., 15 tables, VIII+338 pp.

On the development and life cycle of Camallanus anabantis (Nematoda: Camallanidae), a parasite of the climbing perch, Anabas testudineus

Nimai C. De

Folia Parasitologica 46[3] 205-215 (1999)

The developmental stages and life cycle of the nematode Camallanus anabantis Pearse, 1933 an intestinal parasite of Anabas testudineus (Bloch) are described. The copepod Mesocyclops leuckarti (Claus) was used as experimental intermediate host. After being ingested by the copepods the nematode first-stage larvae enter its haemocoel, where they moult twice, 4 d.p.i. and 11 d.p.i., at 21-26°C, respectively to become the infective third-stage larvae. The definitive fish hosts become infected when feeding on copepods harbouring infective larvae. In the fish host's intestine the larvae undergo two more moults, the third on day 15 p.i....

Myleusnema brasiliense sp. n. (Nematoda: Kathlaniidae), a new intestinal parasite of the serrasalmid fish Myleus sp. in Brazil

František Moravec, Vernon E. Thatcher

Folia Parasitologica 46[3] 216-220 (1999)

A new kathlaniid nematode, Myleusnema brasiliense sp. n., is described from the intestine of a characoid fish, Myleus sp. (Serrasalmidae, Cypriniformes), from Brazil. It differs from the only other congeneric species Myleusnema bicornis Moravec et Thatcher, 1996 mainly in having an unseparated cephalic portion of the body, lips provided with flanges with numerous spike-like appendages, deirids situated more posteriorly, more numerous (8 pairs) subventral preanal papillae in the male, and distinctly longer spicules (1.58-1.70 mm). The species description is based on light microscopic and scanning electron microscopic (SEM) observations.

Aspects of the morphology and a review of the taxonomic status of three species of the genus Chonopeltis (Crustacea: Branchiura) from the Orange-Vaal and South West Cape River Systems, South Africa

Liesl L. Van As, Jo G. Van As

Folia Parasitologica 46[3] 221-228 (1999)

Comparative scanning electron microscopical studies were carried out on Chonopeltis australis Boxshall, 1976 collected from different localities in the Orange-Vaal River System in South Africa and on material of Chonopeltis minutus Fryer, 1977 and Chonopeltis australissimus Fryer, 1977 on loan from the Albany Museum, Grahamstown. This elucidates the fine structure of morphological features, which are of taxonomic importance and illustrates the significance of the copulatory structures on the legs as a taxonomic tool. It was also concluded that C. australissimus is the same as C. minutus, with C. australissimus...

A redescription of the adult male and praniza of Gnathia africana Barnard, 1914 (Crustacea, Isopoda, Gnathiidae) from southern Africa

Nico J. Smit, Jo G. Van As, Linda Basson

Folia Parasitologica 46[3] 229-240 (1999)

A redescription of the adult male and praniza of Gnathia africana Barnard, 1914 is provided from material collected at three localities along the South African coast and from syntypes and other material deposited by the original author. This redescription is based on light and scanning electron microscopy.