Folia Parasitologica, vol. 47 (2000), issue 1

Phylogenetic analysis of Trypanosomatina (Protozoa: Kinetoplastida) based on minicircle conserved regions

Vyacheslav Yurchenko, Alexander A. Kolesnikov, Julius Lukeš

Folia Parasitologica 47[1] 1-5 (2000) | 10.14411/fp.2000.001

Phylogenetic relationships within the suborder Trypanosomatina were inferred from the kinetoplast DNA minicircle conserved region sequences. Trees built using distance-matrix (Neighbor-Joining) and maximum parsimony methods showed that the minicircle conserved regions (CRs) provide a sensitive and specific molecular marker suitable for phylogenetic analyses of subspecies and strains of trypanosomatid flagellates, as testified by the subdivision of the genus Leishmania into the subgenera Leishmania, Viannia and Sauroleishmania. However, since Phytomonas and monogenetic parasites of insects represent the earliest diverging...

Preference of female rats for the odours of non-parasitised males: the smell of good genes?

Charlene Willis, Robert Poulin

Folia Parasitologica 47[1] 6-10 (2000) | 10.14411/fp.2000.002

Many animals obtain reliable information about potential mates, including whether they are parasitised or not, mostly from olfactory cues in urine. Previous experiments with rodents have shown that females can detect parasites in males that are potentially transmissible during copulation, so that females can directly avoid infection by discriminating against parasitised males. Here, using choice tests, we examine whether female rats can distinguish males infected with the tapeworm Hymenolepis diminuta Rudolphi, 1819, a parasite with a complex life cycle and thus not directly transmissible among rats. Female rats tended to spend more time investigating...

Neopolystoma liewi sp. n. (Monogenea: Polystomatidae) from the eye of the Malayan box turtle (Cuora amboinensis)

Louis H. du Preez, L.H.S. Lim

Folia Parasitologica 47[1] 11-16 (2000) | 10.14411/fp.2000.003

Neopolystoma liewi sp. n. is described from the conjunctival cavity of the Malayan box turtle Cuora amboinensis (Daudin, 1802), in Peninsular Malaysia. This is the first record of Neopolystoma in Malaysia and the fourth polystomatid species described from C. amboinensis. Of the 27 Malayan box turtles examined, 8 were found to be infected. A maximum of 2 parasites per eye and 4 individuals per host was recorded. N. liewi sp. n. differs from all other members of the genus by possessing few and short genital spines and small marginal hooks. The oncomiracidium has 64 ciliated cells arranged symmetrically about the sagittal...

Comparative infectivity of Fasciola hepatica metacercariae from isolates of the main and secondary reservoir animal host species in the Bolivian Altiplano high human endemic region

María Adela Valero, Santiago Mas-Coma

Folia Parasitologica 47[1] 17-22 (2000) | 10.14411/fp.2000.004

Fascioliasis due to Fasciola hepatica (Linnaeus, 1758) is an endemic disease on the Northern Bolivian Altiplano, where human prevalences and intensities are the highest known, sheep and cattle are the main reservoir hosts, and pigs and donkeys the secondary ones. Investigations were carried out to study the viability of metacercariae experimentally obtained from eggs shed by naturally infected Altiplanic sheep, cattle, pigs and donkeys. A total of 157 Wistar rats were infected with doses of 5, 10, 20 and 150 metacercariae. Metacercariae aged for different number of weeks were used to analyse the influence of age on their viability. The number...

Morphology of Bertiella studeri (Blanchard, 1891) sensu Stunkard (1940) (Cestoda: Anoplocephalidae) of human origin and a proposal of criteria for the specific diagnosis of bertiellosis

María Teresa Galán-Puchades, Màrius Vicent Fuentes, Santiago Mas-Coma

Folia Parasitologica 47[1] 23-28 (2000) | 10.14411/fp.2000.005

Human material of an African specimen of Bertiella studeri (Blanchard, 1891), a typical intestinal cestode of monkeys, is described. Mature, postmature and gravid proglottides, and eggs, previously inadequately figured, are illustrated and photographed. The description of the species agrees with that provided by Stunkard (1940). A comparative study with other descriptions of the species is made in an attempt to clarify previous findings. The morphological differences reported in various earlier descriptions of the species suggest that B. studeri should be regarded as a "B. studeri species complex". Improvements are required in...

Ultrastructure of the scolex, rhyncheal system and bothridial pits of Otobothrium mugilis (Cestoda: Trypanorhyncha)

Malcolm K. Jones

Folia Parasitologica 47[1] 29-38 (2000) | 10.14411/fp.2000.006

The ultrastructure of the scolex tegument, bothridial pits (=ciliated pits) and rhyncheal system of Otobothrium mugilis Hiscock, 1954 is described from plerocerci collected from the teleosts Arius graeffei Kner et Steindachner and Mugil cephalus Linnaeus. Scanning electron microscopy revealed that filamentous microtriches with shortened caps are abundant across the entire surface of the tegument. Palmate microtriches are dominant on the bothridia and their margins. The surfaces of bothridial pits were covered with large bifid microtriches. The bothridial pits are strongly muscularised invaginations of the tegument. Nervous tissues...

The effect of condensed tannins from seven herbages on Trichostrongylus colubriformis larval migration in vitro

Abdul L. Molan, Garry C. Waghorn, Beyng R. Min, Warren C. McNabb

Folia Parasitologica 47[1] 39-44 (2000) | 10.14411/fp.2000.007

The effects of condensed tannins (CT) extracted from seven forages on the motility of the economically important nematode, Trichostrongylus colubriformis (Giles, 1892), were evaluated by using a larval migration inhibition (LMI) assay. The assay involved incubation of third stage (L3) exsheathed T. colubriformis larvae with CT extracted from Lotus pedunculatus, Lotus corniculatus, sulla (Hedysarum coronarium), sainfoin (Onobrychis viciifolia), Dorycnium rectum, Dorycnium pentaphyllum and dock (Rumex obtusifolius) and measurement of larval migration through nylon mesh with a 20µm...

Bacillary band ultrastructure of the fish parasite Capillaria pterophylli (Nematoda: Capillariidae)

Zdeňka Žďárská, Jana Nebesářová

Folia Parasitologica 47[1] 45-48 (2000) | 10.14411/fp.2000.008

In Capillaria pterophylli Heinze, 1933, two lateral bacillary bands extend along the whole body in female and male worms. A ventral bacillary band is present in females only. The bacillary bands consist of glandular and non-glandular cells, and in the region between the nerve ring and the end of the stichosome, ciliated sense receptors in tight connection with gland cells are present.

A study of proteases throughout the life cycle of Trichinella spiralis

Rosa Mª Ros-Moreno, Celia Vázquez-López, Consuelo Giménez-Pardo, Cristina de Armas-Serra, Filomena Rodríguez-Caabeiro

Folia Parasitologica 47[1] 49-54 (2000) | 10.14411/fp.2000.009

In the present report we study the proteolytic activity of the excretion-secretion and crude extracts of different stages of Trichinella spiralis (Owen, 1835) Railliet, 1895, (muscle-stage larvae, adult worms before and after mating, and newborn larvae) using natural substrates (structural and hematic mammalian proteins). The analysis of the results allow us to set up a certain stage-specificity, as well as an important relationship between the protease patterns throughout the parasite life cycle and how the parasite may overcome both mechanical and humoral barriers within the host. Muscle-stage larvae present a great activity against structural...

Analysis of intestinal intraepithelial lymphocyte populations in experimental Trichinella spiralis infection of mice

Frane Božić, Albert Marinculić, Emir Duraković

Folia Parasitologica 47[1] 55-59 (2000) | 10.14411/fp.2000.010

The potential role of intestinal intraepithelial lymphocytes (i-IELs) in the generation of host protective immunity after helminth infection was investigated using the Trichinella spiralis (Owen, 1835)/mouse model. In this study we found a significant rise of TCRγδ+ i-IELs (P < 0.001) concurrent with the jejunal goblet cells (GC) hyperplasia in T. spiralis-infected C57BL mice on day 4 p.i. However, no direct relationship between the kinetics of the increase in TCRγδ+ i-IELs and T. spiralis expulsion was observed in infected mice. Taken together, these results implicate that γδ...

F. Moravec: Nematodes of Freshwater Fishes of the Neotropical Region.

R.C. Anderson

Folia Parasitologica 47[1] 60 (2000) | 10.14411/fp.2000.011

Academia, Praha, 1998, ISBN 80-200-0705-9, hard cover. 177 figs., 464 pp.

A redescription of the adult male of Caecognathia cryptopais (Barnard, 1925) (Crustacea: Isopoda: Gnathiidae) from southern Africa

Nico J. Smit, Linda Basson, Jo G. Van As

Folia Parasitologica 47[1] 61-66 (2000) | 10.14411/fp.2000.012

A redescription of the adult male of Caecognathia cryptopais (Barnard, 1925) is provided from syntypes and other material deposited in the South African Museum. The generic status of Caecognathia cryptopais is also revised. This redescription is based on light and scanning electron microscopy.

Comparison of the protein profiles of salivary gland extracts derived from three species of unfed and partially fed ixodid ticks analysed by SDS-PAGE

Mirko Slovák, Valéria Hajnická, Milan Labuda, Norbert Fuchsberger

Folia Parasitologica 47[1] 67-71 (2000) | 10.14411/fp.2000.013

Salivary gland extracts (SGE) from unfed and 5 days fed adult female Ixodes ricinus (Linnaeus, 1758); Haemaphysalis inermis (Birula, 1895) and Dermacentor reticulatus (Fabricius, 1794) ticks were prepared. The protein content after feeding increased by 10.6, 8.7 and 6.8 times, respectively. Extracts were equilibrated to the same protein content and submitted to SDS-polyacrylamide gel electrophoresis followed by computer analysis of the scanned gels. Relative differences in protein profiles of extracts obtained from unfed and partially fed ticks were found in all species and some of them were similar in all three species used in...

W.C. Marquardt, R.S. Demaree, R.B. Grieve: Parasitology and Vector Biology.

Oleg Ditrich

Folia Parasitologica 47[1] 72 (2000) | 10.14411/fp.2000.014

Second edition. Harcourt Academic Press, San Diego - London - Boston - New York - Sydney - Tokyo - Toronto 1999, ISBN: 0-12-473275-5, hard cover. 400+ photos and drawings, 702 pp. US Price: $84.95, Europe: ₤51.95

Notes on mites of the family Myobiidae (Acari: Prostigmata) parasitising rodents (Mammalia: Rodentia) in Iran

Andre Bochkov, Masoud Arbobi, Vladimir Malikov

Folia Parasitologica 47[1] 73-77 (2000) | 10.14411/fp.2000.015

Six mite species of the family Myobiidae, Radfordia (Austromyobia) persica sp. n., Radfordia (Austromyobia) merioni Bochkov, Dubinina et Chirov, 1990, Radfordia (Radfordia) acomys Fain et Lukoschus, 1977, Radfordia (Radfordia) affinis (Poppe, 1896), Radfordia (Graphiurobia) dyromys Fain et Lukoschus, 1973, and Myobia (Myobia) murismusculi (Schrank, 1781) were found in Iran on the rodents Gerbillus cheesmani Thomas, Meriones libycus Lichtenstein, Acomys cahirinus (Desmarest), Mus musculus L., Dryomys...

Kabatana gen. n., new name for the microsporidian genus Kabataia Lom, Dyková et Tonguthai, 1999

Jiří Lom, Iva Dyková, Kamonporn Tonguthai

Folia Parasitologica 47[1] 78 (2000) | 10.14411/fp.2000.016

Detection of spirochetes in, and isolation from, culicine mosquitoes

Yibayiri O. Sanogo, Jiří Halouzka, Zdeněk Hubálek, Miroslav Němec

Folia Parasitologica 47[1] 79-80 (2000) | 10.14411/fp.2000.017