Folia Parasitologica, vol. 51 (2004), issue 4

Susceptibility of IFN-γ or IL-12 knock-out and SCID mice to infection with two microsporidian species, Encephalitozoon cuniculi and E. intestinalis

Jiří Salát, Bohumil Sak, Thuy Le, Jan Kopecký

Folia Parasitologica 51[4] 275-282 (2004) | DOI: 10.14411/fp.2004.033

Susceptibility of three strains of immunodeficient mice to two related microsporidian species Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923 and Encephalitozoon intestinalis (Cali, Kotler et Orenstein, 1993) was compared. While both, severe combined immunodeficient (SCID) and interferon-gamma knock-out (IFN-γ KO) mice, succumbed to either intraperitoneal (i.p.) or peroral (p.o.) (natural) infection with both parasites, only i.p. infection with E. cuniculi killed interleukin-12 knock-out (IL-12 KO) mice. IFN-γ KO mice died earlier than SCID mice. Adoptive transfer of naive splenocytes from IFN-γ KO...

Two new species of Isospora (Apicomplexa: Eimeriidae) from geckoes of the genus Rhacodactylus (Sauria: Gekkonidae) endemic to New Caledonia

David Modrý, Miloslav Jirků, Milan Veselý

Folia Parasitologica 51[4] 283-286 (2004) | DOI: 10.14411/fp.2004.034

Coprological examination of New Caledonian geckoes of the genus Rhacodactylus Fitzinger, 1843 revealed two new species of coccidia. Isospora leachiani sp. n. from R. leachianus (Cuvier, 1829) has oval, colourless oocysts, measuring 21-26 × 16-18.5 µm. Sporocysts are ellipsoidal, 11-12.5 × 6.5-8 µm, with distinct Stieda and substieda bodies. Oocysts of Isospora sykorai sp. n. from R. ciliatus (Guichenot, 1866) are elongately oval to cylindrical, 20-23.5 × 11-14 µm; sporocysts of this species are ellipsoidal, 10-11.5 × 7-8 µm, with a slightly pointed end and Stieda and substieda bodies. Infected...

Fine structure and function of the genital pore of the female of Intoshia variabili (Orthonectida)

George S. Slyusarev

Folia Parasitologica 51[4] 287-290 (2004) | DOI: 10.14411/fp.2004.035

The genital pore of the female of Intoshia variabili Аleksandrov et Sljusarev, 1992 is located on a transverse ring of non-ciliated cells. Before copulation it is occupied by 6-7 cells that develop in the epithelium and that for a time are beneath the surface. When fully developed, these cells contain many electron-dense granules and their apical surface is covered with numerous microvilli. After copulation the granules disappear and the apical surface becomes smooth. The cells still form a plug closing the opening. After one larva pushes the pore cells out and escapes, others follow.

Monogenoids from the gills of spiny eels (Teleostei: Mastacembelidae) in India and Iraq, proposal of Mastacembelocleidus gen. n., and status of the Indian species of Actinocleidus, Urocleidus and Haplocleidus (Monogenoidea: Dactylogyridae)

Delane C. Kritsky, K.C. Pandey, Nirupama Agrawal, Shamall M.A. Abdullah

Folia Parasitologica 51[4] 291-298 (2004) | DOI: 10.14411/fp.2004.036

Mastacembelocleidus gen. n. (Monogenoidea: Dactylogyridae) is proposed to include two species collected and redescribed from spiny eels (Mastacembelidae) in India and Iraq: Mastacembelocleidus bam (Tripathi, 1959) comb. n. (syn. Ancyrocephalus bam Tripathi, 1959) from the gills of Macrognathus pancalus (new host record) and Macrognathus aculeatus (Synbranchiformes: Mastacembelidae) from Lucknow, India; and Mastacembelocleidus heteranchorus (Kulkarni, 1969) comb. n. (syn. Urocleidus heteranchorus Kulkarni, 1969) from the gills of Mastacembelus armatus from Lucknow, India, and Mastacembelus mastacembelus...

Fish trypanosomes from the Okavango Delta, Botswana

Nico J. Smit, Jo G. Van As, Angela J. Davies

Folia Parasitologica 51[4] 299-303 (2004) | DOI: 10.14411/fp.2004.037

During 2001 and 2002, blood smears from 37 of 120 fishes belonging to 10 species captured in the Okavango Delta region of Botswana, were found to harbour trypanosomes. These trypanosomes displayed differing staining properties, were morphometrically variable, and ranged in total length from 29.5 to 80.8 µm. Mixed populations of the smaller and larger trypanosomes were found in most fish examined. Despite variations in size and appearance, these specimens are tentatively identified as Trypanosoma mukasai Hoare, 1932, likely adding another 9 new hosts to those known for this parasite. It is possible that Trypanosoma clariense Pienaar,...

Two new species of Myliocotyle (Monogenea: Monocotylidae) from the gills of Aetomylaeus maculatus and A. nichofii (Elasmobranchii: Myliobatidae) from Sarawak, Borneo, Malaysia

Leslie A. Chisholm, Ian D. Whittington

Folia Parasitologica 51[4] 304-310 (2004) | DOI: 10.14411/fp.2004.038

Myliocotyle borneoensis sp. n. and M. multicrista sp. n. (Monocotylidae: Heterocotylinae) are described from the gills of the mottled eagle ray, Aetomylaeus maculatus (Gray), and the banded eagle ray A. nichofii (Bloch et Schneider) (Myliobatidae), respectively, collected from the northern coast of Malaysian Borneo. These are the first monogeneans to be described on elasmobranchs from Borneo. The formerly monotypic Myliocotyle (for M. pteromylaei) was distinguished from other monocotylids by the distribution and morphology of the eight sclerotised dorsal haptoral accessory structures and the morphology of the...

Functional and phylogenetic components in cercarial nervous systems

Tami Bogéa

Folia Parasitologica 51[4] 311-319 (2004) | DOI: 10.14411/fp.2004.039

Studies involving comparisons of taxa that vary in their degree of relatedness may allow the distinction of functional and phylogenetic components in cercarial sensory systems. In this study, cercariae of allocreadiids Bunodera Railliet, 1896 and Crepidostomum Braun, 1900, lecithodendriid Allassogonoporus Olivier, 1938 and opecoelid Allopodocotyle Pritchard, 1966 were compared as regards ultrastructure and chaetotaxy of sensory receptors as well as neuromorphology. Cercariae were treated with acetylthiocholine iodide and silver nitrate and some were processed for scanning and transmission electron microscopy. The types of...

The life cycle of Paracardicoloides yamagutii Martin, 1974 (Digenea: Sanguinicolidae)

Matthew J. Nolan, Thomas H. Cribb

Folia Parasitologica 51[4] 320-326 (2004) | DOI: 10.14411/fp.2004.040

The sanguinicolids Paracardicoloides yamagutii Martin, 1974 and Plethorchis acanthus Martin, 1975 were obtained from their definitive hosts, Anguilla reinhardtii Steindachner and Mugil cephalus Linnaeus (respectively) in the tributaries of the Brisbane River, Queensland, Australia. Two putative sanguinicolid cercariae were collected from a hydrobiid gastropod, Posticobia brazieri Smith, in the same waters. The two cercariae differ markedly in size and the form of their sporocysts. Both putative cercariae develop in the digestive gland of Po. brazieri. The ITS2 rDNA region from these sanguinicolids and a Clinostomum...

Arlenelepis harpiprioni gen. et sp. n. (Cestoda: Dilepididae) from Harpiprion caerulescens (Vieillot) (Aves: Threskiornithidae) in Paraguay

Boyko B. Georgiev, Claude Vaucher

Folia Parasitologica 51[4] 327-332 (2004) | DOI: 10.14411/fp.2004.041

Arlenelepis harpiprioni gen. et sp. n. (Cyclophyllidea, Dilepididae) is described from the plumbeous ibis Harpiprion caerulescens (Vieillot) (Ciconiiformes, Threskiornithidae) in Province Concepción, Paraguay. This cestode is characterised by a very small body (not exceeding 5 mm in length) consisting of about 30 proglottides, musculo-glandular rostellar apparatus, rostellar hooks arranged in two regular rows, few testes (7-10 in number) situated mostly in a post-ovarian group but one testis pre-ovarian, a large oval cirrus sac reaching antiporal osmoregulatory canals, massive cirrus armed with needle-shaped and thorn-shaped spines, long...

Ultrastructure of the apical glandular region of the scolex of Proteocephalus torulosus (Cestoda: Proteocephalidae)

Zdeňka Žďárská, Tomáš Scholz, Jana Nebesářová

Folia Parasitologica 51[4] 333-338 (2004) | DOI: 10.14411/fp.2004.042

In the apical glandular region of the adult Proteocephalus torulosus (Batsch, 1786), two types of eccrine gland cells are present. The first type of unicellular gland produces large electron-dense granules of various sizes. The second type contains small electron-dense granules. Most cells form glands with large granules; glands with small granules are infrequent. The secretion of both types of gland cells is concentrated in the apical parts of the cyton and in the ducts opening to the exterior. On the scolex of P. torulosus, there are regional structural differences of the microthrix border. The apical glandular region bears filamentous...

Redescription of Dracunculus globocephalus Mackin, 1927 (Nematoda: Dracunculidae), a parasite of the snapping turtle, Chelydra serpentina

František Moravec, M.D. Little

Folia Parasitologica 51[4] 339-345 (2004) | DOI: 10.14411/fp.2004.043

Dracunculus globocephalus Mackin, 1927 (Nematoda: Dracunculoidea) is redescribed from specimens collected from the mesentery of the snapping turtle, Chelydra serpentina (L.), in Louisiana, USA. The use of scanning electron microscopy, applied for the first time in this species, made it possible to study details in the structure of the cephalic end and the arrangement of male caudal papillae that are difficult to observe under the light microscope. This species markedly differs from all other species of Dracunculus in having the spicules greatly unequal in size and shape, in the absence of a gubernaculum, and in the disposition...

New data on the morphology and systematic status of Spinitectus petrowi and Spinitectus gigi (Nematoda: Cystidicolidae) parasitic in catfishes in central China

František Moravec, Pin Nie, Guitang Wang

Folia Parasitologica 51[4] 346-358 (2004) | DOI: 10.14411/fp.2004.044

Two little-known nematode species of the genus Spinitectus Fourment, 1883, S. petrowi Belous, 1965 (prevalence 25%, intensity 1-8) and S. gigi Fujita, 1927 (prevalence 10%, intensity 2-3), were collected from the gastrointestinal tract of the yellow catfish, Pelteobagrus fulvidraco (Richardson), from Liangzihu Lake, Hubei Province, central China, in September of 2002. The light and scanning electron microscopical examination of this material, supplemented by a few museum specimens of S. gigi collected from the catfish Clarias fuscus (Lacépède) in southern China, made it possible to study in detail the...

Chigger mites of the genus Eutrombicula Ewing, 1938 (Acari: Trombiculidae) from Cuba, with the description of three new species

Milan Daniel, Alexandr A. Stekol'nikov

Folia Parasitologica 51[4] 359-366 (2004) | DOI: 10.14411/fp.2004.045

Three new species of chigger mites, Eutrombicula cubensis sp. n. and E. anguliscuta sp. n. from lizards and bats, and E. leiocephali sp. n. from lizards, are described. One species, E. lipovskyana (Wolfenbarger, 1953), is recorded for the first time in Cuba. Data on distribution of E. alfreddugesi (Oudemans, 1910) in Cuba are reported.

Effect of tick salivary gland extract on the cytokine production by mouse epidermal cells

Jitka Pechová, Jan Kopecký, Jiří Salát

Folia Parasitologica 51[4] 367-372 (2004) | DOI: 10.14411/fp.2004.046

Previous studies have demonstrated that both tick saliva and Borrelia burgdorferi sensu lato antigens modulate the cytokine response of the host. In this paper, the effect of salivary gland extract (SGE) from Ixodes ricinus (L., 1758) ticks on cytokine production by primary cultures of mouse epidermal cells stimulated with Borrelia afzelii Canica, Nato, du Merle, Mazie, Baranton et Postic, 1993 spirochetes was analysed. Epidermal cells were derived from C3H/HeN mice, susceptible to Lyme disease, and BALB/c mice, which are resistant. In cultures from C3H/HeN mice, SGE down regulated production of tumour necrosis factor alpha (TNF-α)...

Failed attempt of Cryptosporidium andersoni infection in lambs

Martin Kváč, Oleg Ditrich, Martin Kouba, Bohumil Sak, Jiří Vítovec, Dana Květoňová

Folia Parasitologica 51[4] 373-374 (2004) | DOI: 10.14411/fp.2004.047

Four lambs 4 months old were inoculated with Cryptosporidium andersoni oocysts (bovine isolate) in a dose of 5 × 106 per animal followed later by 1 × 107 oocysts per animal. No animal shed Cryptosporidium oocysts during 56 days post infection, no lamb showed clinical symptoms of cryptosporidiosis and no macroscopic changes were detected in the abomasum immediately after autopsy. Histological examinations did not demonstrate the occurrence of Cryptosporidium in the abomasum and other selected organs.

A.A. Guglielmone, A. Estrada-Peña, J.E. Keirans, R.G. Robbins: Ticks (Acari: Ixodida) of the Neotropical Zoogeographic Region.

František Dusbábek

Folia Parasitologica 51[4] 375 (2004) | DOI: 10.14411/fp.2004.048

A special publication sponsored by International Consortium on Ticks and Tick-borne Diseases (ICTTD-2). Atalanta, Houten, The Netherlands, 2003. ISBN 987-43-6828-4, hardbound, 173 pp. Price not given.