Folia Parasitologica 47[1] 1-5 (2000) | DOI: 10.14411/fp.2000.001

Phylogenetic analysis of Trypanosomatina (Protozoa: Kinetoplastida) based on minicircle conserved regions

Vyacheslav Yurchenko1,3, Alexander A. Kolesnikov1, Julius Lukeš2
1 Department of Molecular Biology, Faculty of Biology, Moscow State University, Moscow 119899, Russia
2 Institute of Parasitology, Academy of Sciences of the Czech Republic, and Faculty of Biology, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic
3 The Picower Institute for Medical Research, 350 Community Drive, Manhasset, NY 11030, USA

Phylogenetic relationships within the suborder Trypanosomatina were inferred from the kinetoplast DNA minicircle conserved region sequences. Trees built using distance-matrix (Neighbor-Joining) and maximum parsimony methods showed that the minicircle conserved regions (CRs) provide a sensitive and specific molecular marker suitable for phylogenetic analyses of subspecies and strains of trypanosomatid flagellates, as testified by the subdivision of the genus Leishmania into the subgenera Leishmania, Viannia and Sauroleishmania. However, since Phytomonas and monogenetic parasites of insects represent the earliest diverging groups, the CRs do not seem to be useful for inference of relationships among major lineages of the order Kinetoplastida.

Keywords: Kinetoplastida, Trypanosomatidae, minicircle, kinetoplast DNA, molecular phylogeny

Received: April 23, 1999; Accepted: November 2, 1999; Published: March 1, 2000  Show citation

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Yurchenko, V., Kolesnikov, A.A., & Lukeš, J. (2000). Phylogenetic analysis of Trypanosomatina (Protozoa: Kinetoplastida) based on minicircle conserved regions. Folia Parasitologica, 47(1), 1-5. doi: 10.14411/fp.2000.001
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