Folia Parasitologica 54[4] 310-312 (2007) | DOI: 10.14411/fp.2007.040

Detection of Anaplasma DNA in Ixodes ricinus ticks: pitfalls

Silvie Šikutová1, Ivo Rudolf1, Maryna Golovchenko2,3, Nataliia Rudenko2,3, Libor Grubhoffer2,3, Zdeněk Hubálek1,4
1 Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Květná 8, 603 65 Brno, Czech Republic
2 Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic
3 Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic
4 Faculty of Science, Masaryk University, Kotlářská 2, 602 00 Brno, Czech Republic

A total of 150 nymphal Ixodes ricinus (L., 1758) (Acari: Ixodidae) from the Czech Republic were examined for Anaplasma phagocytophilum (Foggie, 1949) Dumler et al., 2001 by PCR using EHR521/747 primers: 22 of 50 pools were positive (minimum prevalence, 14.7%). However, sequencing of the PCR products did not show complete homology with A. phagocytophilum (91%) while the closest relationship (95%) was found to "Candidatus Ehrlichia walkerii". The results indicate a need for care in interpretation of Anaplasma PCR results and for PCR optimization for detecting A. phagocytophilum in ticks.

Received: January 30, 2007; Accepted: June 6, 2007; Published: December 1, 2007  Show citation

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Šikutová, S., Rudolf, I., Golovchenko, M., Rudenko, N., Grubhoffer, L., & Hubálek, Z. (2007). Detection of Anaplasma DNA in Ixodes ricinus ticks: pitfalls. Folia Parasitologica, 54(4), 310-312. doi: 10.14411/fp.2007.040
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