Folia Parasitologica, vol. 62 (2015)

Folia Parasitologica 62:002 (2015) | 10.14411/fp.2015.002

Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania

Patrícia Manuela Vieira1, Narcisa Mederle2, Maria Luísa Lobo1, Kálmán Imre3, Ovidiu Mederle4, Lihua Xiao5, Gheorghe Darabus2, Olga Matos1
1 Unidade de Parasitologia Médica, Grupo de Protozoários Oportunistas/VIH e Outros Protozoários, CMDT, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Portugal;
2 Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, Faculty of Veterinary Medicine, Banat's University of Agricultural Sciences and Veterinary Medicine Timisoara, Timisoara, Romania;
3 Department of Food Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, Banat's University of Agricultural Sciences and Veterinary Medicine, Timisoara, Romania;
4 Faculty of Medicine, University of Medicine and Pharmacy, Timisoara, Romania;
5 Division of Foodborne, Waterborne and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA

To investigate the transmission of species of Cryptosporidium Tyzzer, 1907 in Timis County, Romania, 48 isolates of Cryptosporidium coccidia from 11 children, 29 calves and eight pigs were characterised by molecular analysis of two loci (SSU rRNA and 60-kDa glycoprotein gene). Overall, 22 isolates were amplified and sequence analyses revealed that all isolates were Cryptosporidium parvum Tyzzer, 1912. Two subtype families were identified, IIa and IId. Subtype IIdA22G1 (n = 4) was the single C. parvum subtype found in children. Subtypes found in calves included IIdA27G1 (n = 8), a novel subtype, IIdA25G1 (n = 5), IIdA22G1 (n = 2), IIdA21G1a (n = 1), and IIaA16G1R1 (n = 1). Subtype IIdA26G1 was found in a pig. These results were significantly different from previous Romanian reports, as the five subtypes of family IId identified in this study were never identified previously in this country. Thus, cattle may be a source of Cryptosporidium infections for humans and the transmission dynamics of C. parvum in Romania is more complex than previously believed.

Keywords: Cryptosporidium parvum, man, calves, molecular epidemiology, GP60 variability, eastern Europe

Received: January 31, 2014; Accepted: July 23, 2014; Prepublished online: November 26, 2014; Published online: January 1, 2015


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