Folia Parasitologica 47[1] 39-44 (2000) | DOI: 10.14411/fp.2000.007

The effect of condensed tannins from seven herbages on Trichostrongylus colubriformis larval migration in vitro

Abdul L. Molan1, Garry C. Waghorn1, Beyng R. Min1,2, Warren C. McNabb1
1 Nutrition Group, AgResearch, Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand
2 Institute for Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand

The effects of condensed tannins (CT) extracted from seven forages on the motility of the economically important nematode, Trichostrongylus colubriformis (Giles, 1892), were evaluated by using a larval migration inhibition (LMI) assay. The assay involved incubation of third stage (L3) exsheathed T. colubriformis larvae with CT extracted from Lotus pedunculatus, Lotus corniculatus, sulla (Hedysarum coronarium), sainfoin (Onobrychis viciifolia), Dorycnium rectum, Dorycnium pentaphyllum and dock (Rumex obtusifolius) and measurement of larval migration through nylon mesh with a 20µm pore size. At 100 µg ml-1, CT from L. pedunculatus, L. corniculatus, sulla, sainfoin, D. rectum, D. pentaphyllum and dock inhibited 20%, 10%, 15%, 25%, 28%, 32% and 27% of the larvae, respectively from passing through the sieves compared to controls (no CT added). At 1000 µg CT ml-1, CT purified from D. pentaphyllum had the highest inhibitory activity (63%) against 1-month old larvae followed by sainfoin (59%), L. pedunculatus (57%), D. rectum (53%), dock (50%), sulla (40%) and L. corniculatus (37%). Seven-month old larvae were more sensitive to the action of CT than 1-month old larvae (P < 0.001). Addition of 2 µg polyethylene glycol ([PEG] per µg CT; to remove the effect of CT) eliminated 81-93% of the CT activity (P < 0.001) compared to incubations without PEG. The impact of CT on larval migration suggests a possible role for these plants in ruminant diets as a means to reduce dependence upon proprietary anthelmintics.

Keywords: Trichostrongylus colubriformis, nematode larvae, paralysis, condensed tannins Lotus corniculatus, sulla (Hedysarum coronarium), sainfoin (Onobrychis viciifolia), Dorycnium rectum, Dorycnium

Received: April 20, 1999; Accepted: July 1, 1999; Published: March 1, 2000  Show citation

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Molan, A.L., Waghorn, G.C., Min, B.R., & McNabb, W.C. (2000). The effect of condensed tannins from seven herbages on Trichostrongylus colubriformis larval migration in vitro. Folia Parasitologica, 47(1), 39-44. doi: 10.14411/fp.2000.007
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