Folia Parasitologica 47[1] 67-71 (2000) | DOI: 10.14411/fp.2000.013

Comparison of the protein profiles of salivary gland extracts derived from three species of unfed and partially fed ixodid ticks analysed by SDS-PAGE

Mirko Slovák1, Valéria Hajnická2, Milan Labuda1, Norbert Fuchsberger3
1 Institute of Zoology, Slovak Academy of Sciences, Dúbravská cesta 9, 842 06 Bratislava, Slovakia
2 Institute of Preventive and Clinical Medicine, Limbová 14, 833 01, Bratislava, Slovakia
3 Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 842 46 Bratislava, Slovakia

Salivary gland extracts (SGE) from unfed and 5 days fed adult female Ixodes ricinus (Linnaeus, 1758); Haemaphysalis inermis (Birula, 1895) and Dermacentor reticulatus (Fabricius, 1794) ticks were prepared. The protein content after feeding increased by 10.6, 8.7 and 6.8 times, respectively. Extracts were equilibrated to the same protein content and submitted to SDS-polyacrylamide gel electrophoresis followed by computer analysis of the scanned gels. Relative differences in protein profiles of extracts obtained from unfed and partially fed ticks were found in all species and some of them were similar in all three species used in the study. Results demonstrate that the increase of the protein content in salivary glands during the feeding does not occur proportionally. Some proteins are synthesised preferentially (67.1 kDa, 13.5 kDa) but other bands (in range of 15-16 kDa) present in the SGE derived from unfed ticks are less discernible in that of fed ticks.

Keywords: tick saliva, SDS-PAGE, Ixodes ricinus, Haemaphysalis inermis, Dermacentor reticulatus

Received: June 1, 1999; Accepted: September 15, 1999; Published: March 1, 2000  Show citation

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Slovák, M., Hajnická, V., Labuda, M., & Fuchsberger, N. (2000). Comparison of the protein profiles of salivary gland extracts derived from three species of unfed and partially fed ixodid ticks analysed by SDS-PAGE. Folia Parasitologica, 47(1), 67-71. doi: 10.14411/fp.2000.013
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