Folia Parasitologica, vol. 61 (2014), issue 2

Ultrastructure and molecular phylogeny of four new species of monoxenous trypanosomatids from flies (Diptera: Brachycera) with redefinition of the genus Wallaceina

Vyacheslav Yurchenko, Jan Votýpka, Martina Tesařová, Helena Klepetková, Natalya Kraeva, Milan Jirků, Julius Lukeš

Folia Parasitologica 61[2] 97-112 (2014) | DOI: 10.14411/fp.2014.023

Four new species of monoxenous kinetoplastid parasites are described from Brachycera flies, namely Wallaceina raviniae Votýpka et Lukeš, 2014 and Crithidia otongatchiensis Votýpka et Lukeš, 2014 from Ecuador, Leptomonas moramango Votýpka et Lukeš, 2014 from Madagascar, and Crithidia pragensis Votýpka, Klepetková et Lukeš, 2014 from the Czech Republic. The new species are described here based on sequence analysis of their spliced leader (SL) RNA, glycosomal glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) and small subunit (SSU) rRNA genes, as well as their morphology and ultrastructure. High-pressure freezing and Bernhard's...

Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro

Renata M. de Muno, Marcos A. Moura, Luciany C. de Carvalho, Sergio H. Seabra, Helene S. Barbosa

Folia Parasitologica 61[2] 113-119 (2014) | DOI: 10.14411/fp.2014.017

Toxoplasma gondii Nicolle et Manceaux, 1908 is an obligate intracellular parasite with the ability to infect mammals and birds. The only definitive hosts for T. gondii are felids, as the parasites form immature oocysts that are shed in the faeces. Here we introduce cat cells as a model for the study of experimental toxoplasmosis. We selected epithelial cells derived from cat kidneys (CRFK) as a target to determine the intracellular fate of bradyzoites of the T. gondii ME49 strain. In parallel, we compared this infection using epithelial cells from the rat intestine (IEC-6), considering the enteroepithelial development that occurs in the...

Neotropical Monogenoidea 59. Polyonchoineans from Characidium spp. (Characiformes: Crenuchidae) from southern Brazil

Walter A. Boeger, Renata C. Ferreira, Rogério T. Vianna, Luciana Patella

Folia Parasitologica 61[2] 120-132 (2014) | DOI: 10.14411/fp.2014.010

Gyrodactylidae and Dactylogyridae (Monogenoidea) are described or reported from three species of Characidium Reinhardt (Crenuchidae), small species of Characiformes, from streams located in southern Brazilian states. Gyrodactylus carolinae sp. n. (Gyrodactylidae) is described from the body surface of Characidium lanei Travassos (type host), C. pterostictum Gomez, and Characidium sp. from streams in the states of Paraná and São Paulo. This new species closely resembles species of Gyrodactylus von Nordmann, 1832 described from species of Poeciliidae, from which it differs by the morphology of the hooks...

Halysioncum kishiense sp. n. and Echinobothrium parsadrayaiense sp. n. (Cestoda: Diphyllidea) from the banded eagle ray, Aetomylaeus cf. nichofii off the Iranian coast of the Persian Gulf

Mahdis Meraji Masouleh Moghadam, Mohammad Haseli

Folia Parasitologica 61[2] 133-140 (2014) | DOI: 10.14411/fp.2014.019

Two new species of two genera of the order Diphyllidea van Beneden in Carus, 1863, Halysioncum Caira, Marques, Jensen, Kuchta et Ivanov, 2013 and Echinobothrium van Beneden, 1849 sensu stricto are described from Aetomylaeus cf. nichofii (Bloch et Schneider) off the Iranian coast of the Persian Gulf. Halysioncum kishiense sp. n. differs from all other congeners in the number of apical hooks with the exception of H. hoffmanorum (Tyler, 2001) and H. pigmentatum (Ostrowski de Núñez, 1971). Halysioncum kishiense sp. n. can be easily differentiated from H. hoffmanorum and H....

Redescription and systematic position of Soricinia tripartita Żarnowski, 1955 (Cestoda: Cyclophyllidea), a cestode species parasitic in shrews of the genus Sorex, including erection of Gulyaevilepis gen. n.

Svetlana A. Kornienko, Rasa Binkienė

Folia Parasitologica 61[2] 141-147 (2014) | DOI: 10.14411/fp.2014.015

Soricinia tripartita Żarnowski, 1955 is redescribed on the basis of specimens from the type host Sorex araneus Linnaeus from Lithuania, Latvia and Russia (Republic of Karelia and Republic of Komi - a new geographical record) as well as from Sorex satunini Ognev and Sorex volnuchini Ognev from Russia (Nalchik Area in the Caucasus Mountains). The strobilar morphology of S. tripartita is compared with that of other hymenolepidid cestodes of shrews with an unarmed scolex and serial development of proglottides in the strobila, i.e. species of Mathevolepis Spassky, 1948, Ditestolepis Soltys, 1952, Spasskylepis...

Metazoan gill parasites of the Atlantic bluefin tuna Thunnus thynnus (Linnaeus) (Osteichthyes: Scombridae) from the Mediterranean and their possible use as biological tags

Jacopo Culurgioni, Salvatore Mele, Paolo Merella, Piero Addis, Vincenza Figus, Angelo Cau, Firdes Saadet Karakulak, Giovanni Garippa

Folia Parasitologica 61[2] 148-156 (2014) | DOI: 10.14411/fp.2014.011

The gills of 63 specimens of the Atlantic bluefin tuna Thunnus thynnus (Linnaeus) (Osteichthyes: Scombridae) from three localities of the Mediterranean (Sardinian, Tyrrhenian and Levantine Seas) were examined for metazoan parasites. The parasite fauna of T. thynnus from the Sea of Sardinia included 11 species: five didymozoid trematodes, three capsalid and one hexostomid monogeneans, and one caligid and one pseudocycnid copepods. Four didymozoids were found in fish from the Levantine Sea and only one didymozoid was recorded in fish from the Tyrrhenian Sea. Dividing the hosts into four size-groups (small, medium-sized, large and extra...

Rhabdochona spp. (Nematoda: Rhabdochonidae) from fishes in the Central African Republic, including three new species

František Moravec, Miloslav Jirků

Folia Parasitologica 61[2] 157-172 (2014) | DOI: 10.14411/fp.2014.022

Based on light and scanning electron microscopical studies, three new, one already known and one not identified species of the nematode genus Rhabdochona Railliet, 1916 are reported from the intestine of freshwater fishes in Dzanga-Sangha Protected Areas (Congo River basin), the Central African Republic: Rhabdochona (Rhabdochona) centroafricana sp. n. from Barbus miolepis Boulenger, R. (R.) marcusenii sp. n. from Marcusenius greshoffii (Schilthuis), R. (Globochona) paski Baylis, 1928 from Phenacogrammus aurantiacus (Pellegrin) (new host record),...

The feather mites of nightjars (Aves: Caprimulgidae), with descriptions of two new species from Brazil (Acari: Xolalgidae, Gabuciniidae)

Fabio Akashi Hernandes

Folia Parasitologica 61[2] 173-181 (2014) | DOI: 10.14411/fp.2014.024

Two new species of feather mites are described from nightjars (Caprimulgiformes: Caprimulgidae) of Brazil: Hartingiella neotropica sp. n. (Xolalgidae) described from Hydropsalis parvula (Gould) and Paragabucinia brasiliensis sp. n. (Gabuciniidae) from H. albicollis (Gmelin). The former differs from the type species by having, in males, the anterior projections on epimerites III towards setae 3b and the adanal shield bearing setae ps3 present; in both sexes, a pair of small sclerites situated posterior to setae se have flat suprategumental processes. Paragabucinia brasiliensis sp....

A rare finding of mites (Arachnida: Acari: Leeuwenhoekiidae) parasitising a whip spider (Arachnida: Amblypygi: Charinidae)

Thiago Gonçalves-Souza, Alessandro P. L. Giupponi, Fabio A. Hernandes3

Folia Parasitologica 61[2] 182-184 (2014) | DOI: 10.14411/fp.2014.016

Twelve larvae of unidentified species of Odontacarus Ewing, 1929 (Acari: Leeuwenhoekiidae) were found parasitising an adult male whip spider Charinus brasilianus Weygoldt (Charinidae) in Santa Teresa, mountainous region of Espírito Santo state, southeastern Brazil. These larvae occurred in the intersegmental membrane of prosoma and legs. This is the first report of ectoparasitic mites infecting a charinid whip spider and the first record of leeuwenhoekiid mites parasitising an invertebrate host. We suggest that future studies are essential to understand the reasons why these events of parasitism are so rare in the order Amblypygi.

Lymnaea cubensis, an experimental intermediate host for Fascioloides magna

Philippe Vignoles, Adam Novobilský, Johan Höglund, Martin Kašný, Jan Pankrác, Gilles Dreyfuss, Jean-Pierre Pointier, Daniel Rondelaud

Folia Parasitologica 61[2] 185-188 (2014) | DOI: 10.14411/fp.2014.020

Single-miracidium infections of Lymnaea cubensis (Pfeiffer) from Guadeloupe with the giant liver fluke Fascioloides magna (Bassi, 1875) (Digenea) were carried out during five successive snail generations to determine if this lymnaeid might sustain complete larval development of the parasite. Controls were constituted by a French population of Galba truncatula (Müller) (a single generation) infected according to the same protocol. It was recorded that prevalence and intensity of F. magna infection in L. cubensis progressively increased from F1 to F5 generations. Cercarial shedding of F. magna was noted only...