Folia Parasitologica, vol. 59 (2012), issue 1

Haematozoans from deep water fishes trawled off the Cape Verde Islands and over the Porcupine Seabight, with a revision of species within the genus Desseria (Adeleorina: Haemogregarinidae)

Angela J. Davies, Shazia Hosein, Nigel R. Merrett

Folia Parasitologica 59[1] 1-11 (2012) | DOI: 10.14411/fp.2012.001

Archived blood smears from 32 of 113 fishes in 18 families and 12 orders, trawled from deep North Atlantic waters off the Cape Verde Islands in 1999 and over the Porcupine Seabight in 2001 were found to harbour haematozoans. These included four species of haemogregarines (Adeleorina, Haemogregarinidae) and a species of trypanosome (Trypanosomatina, Trypanosomatidae) located in Porcupine Seabight fishes. Also present were Haemohormidium-like structures of uncertain status found in samples from this location and from the Cape Verde Islands. Although material was limited, two of the haemogregarines were provisionally named Desseria harriottae...

A new species of Choleoeimeria (Apicomplexa: Eimeriidae) from Oustalet\'s chameleon, Furcifer oustaleti (Sauria: Chamaeleonidae)

Chris T. McAllister

Folia Parasitologica 59[1] 12-14 (2012) | DOI: 10.14411/fp.2012.002

One of three (33%) captive specimens of Oustalet's chameleon, Furcifer oustaleti (Mocquard) originally from Madagascar and housed at the Oklahoma City Zoological Park Herpetarium, Oklahoma County, Oklahoma, USA, was found to be passing an undescribed species of Choleoeimeria in its faeces. Oocysts of Choleoeimeria fischeri sp. n. were cylindroidal, 30.3 × 16.8 (28-34 × 15-18) µm, with a smooth, bilayered wall and a length/width ratio (L/W) of 1.8. A micropyle and oocyst residuum was absent but a fragmented polar granule was often present. Sporocysts were ovoidal, 9.6 × 8.0 (9-10 × 7-9) µm, with an L/W of 1.2. Stieda,...

Genetic characterisation of Giardia duodenalis in dairy cattle in Brazil

Flávio Medeiros Paze Silva, Raimundo Souza Lopes, João Pessoa Araújo Jr.

Folia Parasitologica 59[1] 15-20 (2012) | DOI: 10.14411/fp.2012.003

The intestinal protozoan parasite Giardia duodenalis (Lambl, 1859) Kofoid & Christiansen, 1915 [syn. Giardia intestinalis and Giardia lamblia] has emerged as a widespread enteric pathogen in humans and domestic animals. In recent years, G. duodenalis has been found in cattle worldwide and longitudinal studies have reported cumulative prevalence of 100% in some herds. In the present study, we determined the prevalence and genetic characterisation of G. duodenalis in 200 dairy cattle from 10 dairy farms in São Paulo state, Brazil. All faecal specimens were screened for the presence of G. duodenalis...

Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP)

Xin Hu, Chang-Wang Pan, Ya-Fei Li, Han Wang, Feng Tan

Folia Parasitologica 59[1] 21-26 (2012) | DOI: 10.14411/fp.2012.004

In this study, a loop-mediated isothermal amplification (LAMP) assay was established to detect Toxoplasma gondii DNA in mice infected with T. gondii PRU strain. This LAMP assay was based on the sequence of highly repetitive B1 gene. The detection limit of T. gondii LAMP assay was 1 pg of T. gondii DNA, which was evaluated using 10-fold serially diluted DNA of cultured parasites. The LAMP assay was also highly specific for T. gondii and able to detect T. gondii DNA in urine of mice treated with dexamethasone at 90 day post infection (p.i.), although this assay could not detect the DNA in mice urine 2-6 days...

Redescription of Eimeria dorcadis Mantovani, 1966 (Apicomplexa: Eimeriidae) from the dorcas gazelle (Gazella dorcas) in Saudi Arabia

Osama B. Mohammed, Abdulaziz N. Alagaili, Sawsan A. Omer

Folia Parasitologica 59[1] 27-31 (2012) | DOI: 10.14411/fp.2012.005

Eimeria dorcadis Mantovani, 1966 is redescribed from dorcas gazelle (Gazella dorcas (L.)) from Saudi Arabia. Oocysts were detected in 7 out of 22 faecal samples (32%) using floatation method. The sporulated oocysts are cylindrical, slightly flattened at the micropylar pole, measure in average 32 × 19 μm (27-36 × 16-24 μm), length/width ratio being 1.7 (1.5-2.1). Oocyst wall is 1.2 μm thick, smooth, double-layered; outer layer is slightly thicker, light blue in colour; inner layer brownish, with micropyle in the inner layer and apparently continual outer one, measures 2.2 μm, but lacks a micropylar cap. The sporocyst elongate-ellipsoidal,...

Spermiogenesis and spermatozoon of the liver fluke Mediogonimus jourdanei (Microphalloidea: Prosthogonimidae), a parasite of Myodes glareolus (Rodentia: Cricetidae)

Abdoulaye J.S. Bakhoum, Carlos Feliu, Cheikh Tidiane Bâ, Jordi Miquel

Folia Parasitologica 59[1] 32-42 (2012) | DOI: 10.14411/fp.2012.006

Spermatological characters of the liver fluke Mediogonimus jourdanei Mas-Coma et Rocamora, 1978 were studied by means of transmission and scanning electron microscopy. Spermiogenesis begins with the formation of the differentiation zone containing two centrioles associated with striated rootlets and an intercentriolar body. These two centrioles originate two free flagella that undergo a 90° rotation before fusing with the median cytoplasmic process. Both nuclear and mitochondrial migrations toward the median cytoplasmic process occur before the proximodistal fusion of flagella. Finally, the constriction of the ring of arched membranes gives...

Histochemical demonstration of five enzymes\' activities in Macrogyrodactylus clarii (Monogenea: Gyrodactylidae) from the catfish Clarias gariepinus

Safaa Zaky Arafa, El-Sayed Kasem Abd El-Hady, Samir Ahmed El-Abbassy

Folia Parasitologica 59[1] 43-52 (2012) | DOI: 10.14411/fp.2012.007

Histochemical techniques were applied to whole mounts, to study the distribution of the enzymes alkaline phosphatase, acid phosphatase, adenosine triphosphatase, 5'-nucleotidase and glucose-6-phosphatase in the organs and tissues of a viviparous monogenean, Macrogyrodactylus clarii Gussev, 1961, from the gills of the North African catfish Clarias gariepinus (Burchell) in Egypt. The following organs and tissues were studied: head region, anterior adhesive glands, mouth region, pharynx, intestine, testis, vesicula seminalis, male accessory gland, male accessory reservoir, copulatory organ, receptaculum seminis, egg-cell forming region,...

The microecology of dactylogyrids (Monogenea: Dactylogyridae) on the gills of wild spotted rose snapper Lutjanus guttatus (Lutjanidae) from Mazatlan Bay, Mexico

Lilia C. Soler-Jiménez, Emma J. Fajer-Ávila

Folia Parasitologica 59[1] 53-58 (2012) | DOI: 10.14411/fp.2012.008

The spatial distribution and coexistence of monogenean dactylogyrids was assessed on the gills of 63 specimens of wild spotted rose snapper, Lutjanus guttatus (Steindachner), caught in the Mazatlan Bay, Sinaloa, Mexico. Five species are reported: Euryhaliotrema perezponcei García-Vargas, Fajer-Ávila et Lamothe-Argumedo, 2008, Euryhaliotrematoides sp., Haliotrematoides spinatus Kritsky et Mendoza-Franco, 2009, H. plectridium Kritsky et Mendoza-Franco, 2009, and H. guttati García-Vargas, Fajer-Ávila et Lamothe-Argumedo, 2008. All except E. perezponcei and H. guttati represent new geographical records...

Two new species of Protogyrodactylus (Monogenoidea: Dactylogyridae) from the gills of Gerres nigri (Teleostei: Gerreidae) from Senegal

Walter A. Boeger, Arfang Diamanka, Antoine Pariselle, Luciana Patella

Folia Parasitologica 59[1] 59-63 (2012) | DOI: 10.14411/fp.2012.009

Protogyrodactylus ethiopicus sp. n. and P. kritskyi sp. n. are described from the gills of Gerres nigri Günther (Gerreidae, Perciformes) captured from the estuary of the Sine-Saloum River (Senegal, West Africa). These new species differ from previously described species within the genus by a mid- or dextro-ventral vaginal opening (dextral in all other species). They are part of a morphological species group within Protogyrodactylus Johnston et Tiegs, 1922 that has the tip of the superficial root of the ventral anchor resembling a hook and two anterior projections on the anterior margin of the ventral bar. Protogyrodactylus...

A new species of Neoascarophis (Nematoda: Cystidicolidae) parasitic in Mullus argentinae (Perciformes: Mullidae) from the Atlantic coast of South America

Aldenice N. Pereira, Juan T. Timi, Fabiano M. Vieira, José L. Luque

Folia Parasitologica 59[1] 64-70 (2012) | DOI: 10.14411/fp.2012.010

A new nematode species (Neoascarophis mariae n. sp.) is described based on specimens collected from the Argentine goatfish Mullus argentinae (Hubbs et Marini) from coastal waters off the state of Rio de Janeiro, Brazil. In the genus, the new species belongs to the group of species with females that have the vulva near the posterior end of the body. Only males of Neoascarophis longispicula Moravec et Klimpel, 2009 are known and can be distinguished from those of the new species by their larger body, developed and somewhat dorsoventrally expanded flat inner part of the pseudolabia, bifurcate deirids and larger spicules (the left...

Two species of philometrid nematodes (Philometridae) from marine fishes off Japan, including Philometroides branchiostegi sp. n. from Branchiostegus japonicus (Malacanthidae)

Franti¹ek Moravec, Kazuya Nagasawa, Kenji Nohara

Folia Parasitologica 59[1] 71-78 (2012) | DOI: 10.14411/fp.2012.011

Gravid females of two species of philometrid nematodes (Philometridae) were collected from marine perciform fishes in Japanese waters, mainly from the southern Sea of Japan. Based on light microscopy and scanning electron microscopy examinations, the previously described but poorly known species Philometra cryptocentri Yamaguti, 1961 is redescribed from specimens recovered from the abdominal cavity of Acanthogobius flavimanus (Temminck et Schlegel), Pterogobius elapoides (Günther) and P. zonoleucus Jordan et Snyder (all Gobiidae) (all new host records); the number (14) and arrangement of cephalic papillae in this species...