Folia Parasitologica, vol. 61 (2014), issue 1

Morphological and ultrastructural redescription of Chloromyxum leydigi Mingazzini, 1890 (Myxozoa: Myxosporea), type species of the genus, infecting the gall bladder of the marine cartilaginous fish Torpedo marmorata Risso (Chondrichthyes: Torpedinidae), from the Portuguese Atlantic coast

Sónia Rocha, Graça Casal, Saleh Al-Quraishy, Carlos Azevedo

Folia Parasitologica 61[1] 1-10 (2014) | DOI: 10.14411/fp.2014.006

Chloromyxum leydigi Mingazzini, 1890, the type species of Chloromyxum Mingazzini, 1890, is redescribed based on material found in the gall bladder of the cartilaginous fish Torpedo marmorata Risso collected from the Portuguese Atlantic coast and its sporogonic development is described. Plasmodia and mature spores were floating free in the bile. Plasmodia are polysporic and highly polymorphic in shape and organization. Mature spores are spherical to subspherical with a pointed anterior end, measuring 12.3 ± 0.5 μm in length and 9.0 ± 0.5 μm in width. The spore wall is composed of two asymmetric shell valves, each bearing...

Description of Sarcocystis lari sp. n. (Apicomplexa: Sarcocystidae) from the great black-backed gull, Larus marinus (Charadriiformes: Laridae), on the basis of cyst morphology and molecular data

Petras Prakas, Liuda Kutkienė, Dalius Butkauskas, Aniolas Sruoga, Meèislovas ®alakevièius

Folia Parasitologica 61[1] 11-17 (2014) | DOI: 10.14411/fp.2014.002

A morphological type of Sarcocystis cysts found in one of two examined great black-backed gull, Larus marinus (Linnaeus) (Laridae), is considered to represent a new species for which the name Sarcocystis lari sp. n. is proposed and its description is provided. The cysts are ribbon-shaped, very long (the largest fragment found was 6 mm long) and relatively narrow (up to 75 μm). Under a light microscope the cyst wall reaches up to 1 μm and seems to be smooth. Using a computerized image analysis system, knolls, which resemble protrusions on the wall surface, are visible. Lancet-shaped cystozoites measure in average 6.9...

The first report of Toxoplasma gondii antibodies in free-living European bison (Bison bonasus bonasus Linnaeus)

Anna C. Majewska, Anna Werner, W³adys³aw Cabaj, Bo¿ena Moskwa

Folia Parasitologica 61[1] 18-20 (2014) | DOI: 10.14411/fp.2014.012

Toxoplasma gondii Nicolle et Manceaux, 1908 is an apicomplexan parasite with a worldwide distribution. It is of great medical and veterinary importance because it may cause abortion or congenital disease in its intermediate hosts, including man. The European bison, the largest herbivorous animal in Europe, is a species that has been saved from extinction. Twenty-four of 95 examined sera of the European bison (Bison bonasus bonasus) from the Bia³owie¿a Forest, Poland collected from 2008 to 2011 were found to be positive for the presence of T. gondii-specific IgG antibodies using a direct agglutination test, with the antibody titre in positive...

A review of the genus Paramoniezia Maplestone et Southwell, 1923 (Cestoda: Anoplocephalidae), with a new genus, Phascolocestus, from wombats (Marsupialia) and redescriptions of Moniezia mettami Baylis, 1934 and Moniezia phacochoeri (Baylis, 1927) comb. n. from African warthogs (Artiodactyla)

Ian Beveridge

Folia Parasitologica 61[1] 21-33 (2014) | DOI: 10.14411/fp.2014.008

Paramoniezia suis Maplestone et Southwell, 1923 is redescribed from the type and only specimen, and is considered to be a genus inquirendum and species inquirenda, possibly based on a host misidentification. Paramoniezia phacochoeri Baylis, 1927 is redescribed from new material from Phacochoerus africanus (Gmelin) from South Africa and is transferred to Moniezia Blanchard, 1891 as M. phacochoeri (Baylis, 1927) comb. n. A redescription of M. mettami Baylis, 1934, also from Ph. africanus, establishes the independence of the two congeneric species parasitizing warthogs. A new genus, Phascolocestus,...

Detection of antibodies to Neospora caninum in moose (Alces alces): the first report in Europe

Bo¿ena Moskwa, Katarzyna Go¼dzik, Justyna Bieñ, Aleksandra Kornacka, Aleksandra Cybulska, Katarína Reiterová, W³adys³aw Cabaj

Folia Parasitologica 61[1] 34-36 (2014) | DOI: 10.14411/fp.2014.014

Neospora caninum Dubey, Carpenter, Speer, Topper et Uggla, 1988 is a protozoan parasite originally reported as a major cause of bovine abortions worldwide. It is documented that the parasite is widely spread among non-carnivorous cervids. The purpose of this study was to investigate the seroprevalence of N. caninum in moose (Alces alces Linnaeus). Blood samples collected in 2010 and 2012 in the northeastern Poland were tested for antibodies to N. caninum by agglutination test (NAT), a commercial competitive screening enzyme-linked immunosorbent assay (cELISA) and enzyme-linked immunoassay (ELISA). Sera that gave a positive result...

Philometrid nematodes (Philometridae) from marine fishes off the northern coast of Australia, including three new species

Franti¹ek Moravec, Ben K. Diggles

Folia Parasitologica 61[1] 37-54 (2014) | DOI: 10.14411/fp.2014.007

Based on light and scanning electron microscopical studies, the following nine species of Philometridae (Nematoda: Dracunculoidea) are described from female worms parasitizing marine perciform fishes belonging to six families off the northern coast Australia (near Darwin): Philometra australiensis sp. n. from the swimbladder of the king threadfin Polydactylus macrochir (Günther) (Polynemidae); P. epinepheli Dewi et Palm, 2013 from the operculum of the orange-spotted grouper Epinephelus coioides (Hamilton) (Serranidae); Philometra johnii Moravec et Ali, 2013 from the gonad of the croaker Johnius sp. (Sciaenidae);...

A new species of Comephoronema (Nematoda: Cystidicolidae) from the squirrelfish Holocentrus adscensionis (Beryciformes: Holocentridae) off Brazil

Felipe Bisaggio Pereira, Aldenice de Nazaré Pereira, José Luis Luque

Folia Parasitologica 61[1] 55-62 (2014) | DOI: 10.14411/fp.2014.001

A new nematode species, Comephoronema multipapillatum sp. n. (Cystidicolidae), is described from the anterior intestine and caecum of the squirrelfish Holocentrus adscensionis (Osbeck) (Beryciformes: Holocentridae) collected in Angra dos Reis, State of Rio de Janeiro, offshore Brazil. The new species was placed in Comephoronema Layman, 1933 by having an oval oral aperture, four submedian labia, four bilobed submedian sublabia, two narrow lateral pseudolabia and four single cephalic papillae, but mainly by numerous precloacal papillae in males (more than six pairs), in which it differs from species of the otherwise morphologically...

Capillostrongyloides morae sp. n. (Nematoda: Capillariidae) from deep-sea fish (Teleostei, Moridae) in the western Mediterranean Sea

David González-Solís, Maite Carrassón, Ana Pérez-del-Olmo

Folia Parasitologica 61[1] 63-68 (2014) | DOI: 10.14411/fp.2014.003

A new capillariid nematode, Capillostrongyloides morae sp. n., is described from specimens collected from the stomach and intestine of the common mora, Mora moro (Risso), and the Mediterranean codling, Lepidion lepidion (Risso) (both Gadiformes, Moridae), off the Mediterranean coasts of Spain. The new species shows similar morphological features as other congeneric species occurring in freshwater and marine fishes, but it differs in the length of the body and spicules, the size of the caudal bursa, and the presence of an elevated anterior vulvar lip. Capillostrongyloides morae sp. n. is the second species within the genus...

Subulura halli (Ascaridida: Subuluridae) from the endangered great bustard Otis tarda Linnaeus (Aves: Gruiformes) in China

Li-Qiang Du, Zhen Xu, Shun-Cai Li, Liang Li

Folia Parasitologica 61[1] 69-75 (2014) | DOI: 10.14411/fp.2014.004

Subulurid nematodes identified as Subulura halli Barreto, 1918 were collected from the endangered bird Otis tarda Linnaeus (Gruiformes: Otididae) in China. A detailed redescription of the hitherto poorly known species is presented using both light and, for the first time, scanning electron microscopy. Previously unreported and erroneous morphological features of taxonomic significance are revealed. This species can be readily distinguished from its congeners by the relatively long oesophagus (1.47-1.92 mm long, representing 10.6-16.9% of body length), the number and arrangement of male caudal papillae (11 pairs in total, arranged as five...

Cryopreservation of roe deer abomasal nematodes for morphological identification

Paola Beraldo, Ernesto Pascotto

Folia Parasitologica 61[1] 76-80 (2014) | DOI: 10.14411/fp.2014.013

Conventional methods to preserve adult nematodes for taxonomic purposes involve the use of fixative or clearing solutions (alcohol, formaldehyde, AFA and lactophenol), which cause morphological alterations and are toxic. The aim of this study is to propose an alternative method based on glycerol-cryopreservation of nematodes for their subsequent identification. Adults of trichostrongylid nematodes from the abomasum of roe deer (Capreolus capreolus Linnaeus) were glycerol-cryopreserved and compared with those fixed in formaldehyde, fresh and frozen without cryoprotectans. Morphology, transparency and elasticity of the anterior and posterior portion...

Host specificity and metamorphosis of the glochidium of the freshwater mussel Unio tumidiformis (Bivalvia: Unionidae)

Joaquim Reis, Maria João Collares-Pereira, Rafael Araujo

Folia Parasitologica 61[1] 81-89 (2014) | DOI: 10.14411/fp.2014.005

The glochidium larvae of freshwater mussels of the family Unionidae need to find suitable hosts to attach themselves and metamorphose into free-living juveniles. The specificity of the host-parasite relationship was investigated for the Iberian Unio tumidiformis Castro, 1885 by means of experimental infections and also by analyzing naturally infected fish. The process of encapsulation of glochidia was studied using scanning electron microscopy. Unio tumidiformis has proven to be an unusual host-specific unionid mussel, apparently parasitizing only fish of the genus Squalius Bonaparte, 1837. Successful encapsulation or complete...

A new species of the feather mite genus Rhinozachvatkinia (Acari: Avenzoariidae) from Calonectris shearwaters (Procellariiformes: Procellariidae): integrating morphological descriptions with DNA barcode data

Laura M. Stefan, Karen D. McCoy, Sergey Mironov

Folia Parasitologica 61[1] 90-96 (2014) | DOI: 10.14411/fp.2014.009

Rhinozachvatkinia calonectris sp. n., a new species of the feather mite genus Rhinozachvatkinia Mironov, 1989 (Avenzoariidae: Bonnetellinae), is described from two species of shearwaters in the North-East of the Atlantic Ocean, Calonectris edwardsii (Oustalet) (type host) and Calonectris borealis (Cory) (Procellariiformes: Procellariidae). We completed the morphological description of this new feather mite species with sequence data on the mitochondrial cytochrome c oxidase subunit I gene fragment (COI). The full generic status of Rhinozachvatkinia, originally established as a subgenus of Zachvatkinia Dubinin,...